Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay
Summary: Developing a sensitive, low-cost, and easy-to-use point-of-care testing system for genotyping is important for informing treatment decisions and predicting the risk of underlying diseases. Conventional methods normally require complex operational procedures as well as expensive and sophisti...
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doaj-675da049a9834774974163604c02f5ed2020-11-25T00:04:08ZengElsevieriScience2589-00422018-09-017170179Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow AssayChao Zhang0Xiaonan Liu1Yao Yao2Kewu Liu3Wenli Hui4Juanli Zhu5Yaling Dou6Kai Hua7Mingli Peng8Zuankai Wang9Alphonsus J.M. Vermorken10Yali Cui11College of Life Sciences, Northwest University, Xi'an, ChinaCollege of Life Sciences, Northwest University, Xi'an, ChinaShaanxi Provincial Engineering Research Center of Nano-Biomedical Detection, Xi'an, ChinaCollege of Life Sciences, Northwest University, Xi'an, ChinaCollege of Life Sciences, Northwest University, Xi'an, ChinaShaanxi Provincial Engineering Research Center of Nano-Biomedical Detection, Xi'an, ChinaDepartment of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, ChinaCollege of Life Sciences, Northwest University, Xi'an, ChinaShaanxi Provincial Engineering Research Center of Nano-Biomedical Detection, Xi'an, ChinaDepartment of Mechanical Engineering, City University of Hong Kong, Hong Kong, China; Corresponding authorCollege of Life Sciences, Northwest University, Xi'an, ChinaCollege of Life Sciences, Northwest University, Xi'an, China; Shaanxi Provincial Engineering Research Center of Nano-Biomedical Detection, Xi'an, China; Corresponding authorSummary: Developing a sensitive, low-cost, and easy-to-use point-of-care testing system for genotyping is important for informing treatment decisions and predicting the risk of underlying diseases. Conventional methods normally require complex operational procedures as well as expensive and sophisticated instruments. Here, we report a general approach that enables us to detect the genotype of multiple sample types directly without DNA purification. Moreover, the PCR results can be further quantitatively analyzed based on a magnetic lateral flow assay (MLFA) system, which avoids multiple steps needed for conventional nucleic acid biosensors. As a demonstration, we show that three genotypes of aldehyde dehydrogenase 2 (ALDH2) can be identified using a small volume of sample with an accuracy of 100% and a sensitivity of 1.0 × 102 cells/μL, which are better than those of the gold standard methods. We believe that the direct PCR-MLFA system represents a significant advance toward the development of portable, sensitive biomedical platforms. : Clinical Genetics; Analytical Chemistry; Fluidics; Biochemical Analysis Subject Areas: Clinical Genetics, Analytical Chemistry, Fluidics, Biochemical Analysishttp://www.sciencedirect.com/science/article/pii/S2589004218301408 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chao Zhang Xiaonan Liu Yao Yao Kewu Liu Wenli Hui Juanli Zhu Yaling Dou Kai Hua Mingli Peng Zuankai Wang Alphonsus J.M. Vermorken Yali Cui |
spellingShingle |
Chao Zhang Xiaonan Liu Yao Yao Kewu Liu Wenli Hui Juanli Zhu Yaling Dou Kai Hua Mingli Peng Zuankai Wang Alphonsus J.M. Vermorken Yali Cui Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay iScience |
author_facet |
Chao Zhang Xiaonan Liu Yao Yao Kewu Liu Wenli Hui Juanli Zhu Yaling Dou Kai Hua Mingli Peng Zuankai Wang Alphonsus J.M. Vermorken Yali Cui |
author_sort |
Chao Zhang |
title |
Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay |
title_short |
Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay |
title_full |
Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay |
title_fullStr |
Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay |
title_full_unstemmed |
Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay |
title_sort |
genotyping of multiple clinical samples with a combined direct pcr and magnetic lateral flow assay |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2018-09-01 |
description |
Summary: Developing a sensitive, low-cost, and easy-to-use point-of-care testing system for genotyping is important for informing treatment decisions and predicting the risk of underlying diseases. Conventional methods normally require complex operational procedures as well as expensive and sophisticated instruments. Here, we report a general approach that enables us to detect the genotype of multiple sample types directly without DNA purification. Moreover, the PCR results can be further quantitatively analyzed based on a magnetic lateral flow assay (MLFA) system, which avoids multiple steps needed for conventional nucleic acid biosensors. As a demonstration, we show that three genotypes of aldehyde dehydrogenase 2 (ALDH2) can be identified using a small volume of sample with an accuracy of 100% and a sensitivity of 1.0 × 102 cells/μL, which are better than those of the gold standard methods. We believe that the direct PCR-MLFA system represents a significant advance toward the development of portable, sensitive biomedical platforms. : Clinical Genetics; Analytical Chemistry; Fluidics; Biochemical Analysis Subject Areas: Clinical Genetics, Analytical Chemistry, Fluidics, Biochemical Analysis |
url |
http://www.sciencedirect.com/science/article/pii/S2589004218301408 |
work_keys_str_mv |
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