Trace elements storage peculiarities and metallothionein content in human thyroid gland under iodine deficiency euthyroid nodular goiter
Accumulation of iodine and copper in the node, paranodular and contralateral (not affected tissue by node) tissues of thyroid gland in relation to the level of metal-binding proteins, potential antioxidants and oxidative changes in tissue was investigated. To assess the severity of the pathological...
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National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
2014-06-01
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Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Falfushynska_3_14.pdf |
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doaj-6773dc625a1540798d7e63581930abeb2020-11-24T21:23:37ZengNational Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.Ukrainian Biochemical Journal2409-49432413-50032014-06-0186310711310.15407/ubj86.03.107Trace elements storage peculiarities and metallothionein content in human thyroid gland under iodine deficiency euthyroid nodular goiterH. I. Falfushynska0L. L. Gnatyshyna1O. Osadchuk2V. O. Shidlovski3О. B. Stoliar4Ternopil Volodymyr Hnatiuk National Pedagogical University, Ukraine; I.Ya. Horbachevski Ternopil State Medical University, UkraineTernopil Volodymyr Hnatiuk National Pedagogical University, UkraineI.Ya. Horbachevski Ternopil State Medical University, UkraineI.Ya. Horbachevski Ternopil State Medical University, UkraineTernopil Volodymyr Hnatiuk National Pedagogical University, UkraineAccumulation of iodine and copper in the node, paranodular and contralateral (not affected tissue by node) tissues of thyroid gland in relation to the level of metal-binding proteins, potential antioxidants and oxidative changes in tissue was investigated. To assess the severity of the pathological process the molecular markers of cytotoxicity were used. The reduction of total iodine (by 19.5%), increase of inorganic iodine fraction (by 82.4%) and total copper content (twice) in paranodular and nodular tissues compared with contrlateral part have been established. Excess of copper in goitrous-changes tissue was partially accumulated in the metallothioneins. The level of metal-binding form of metallothioneins and reserve of free thiols of these proteins was higher two-three times and lower content of reduced glutathione in node-affected tissue compared to the contralateral part. Signs of cytotoxicity among them: higher cathepsine D free activity (up to 84.6% and 134.4% in paranodular tissue and node respectively) and higher level of DNA strand breaks in the node (up to 22.6%) were observed. In paranodular tissue the range of indices variability compared with parenchyma of contralateral part is shorter than in the node. Thus, under low level of iodine organification and high copper level in goitrous-modified tissue of thyroid gland metallothionein may provide a partial compensatory effect on prooxidative processes.http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Falfushynska_3_14.pdfapoptosiscoppercytotoxicityiodineiodine deficiency nodular colloidal goitermetallothioneinszinc |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
H. I. Falfushynska L. L. Gnatyshyna O. Osadchuk V. O. Shidlovski О. B. Stoliar |
spellingShingle |
H. I. Falfushynska L. L. Gnatyshyna O. Osadchuk V. O. Shidlovski О. B. Stoliar Trace elements storage peculiarities and metallothionein content in human thyroid gland under iodine deficiency euthyroid nodular goiter Ukrainian Biochemical Journal apoptosis copper cytotoxicity iodine iodine deficiency nodular colloidal goiter metallothioneins zinc |
author_facet |
H. I. Falfushynska L. L. Gnatyshyna O. Osadchuk V. O. Shidlovski О. B. Stoliar |
author_sort |
H. I. Falfushynska |
title |
Trace elements storage peculiarities and metallothionein content in human thyroid gland under iodine deficiency euthyroid nodular goiter |
title_short |
Trace elements storage peculiarities and metallothionein content in human thyroid gland under iodine deficiency euthyroid nodular goiter |
title_full |
Trace elements storage peculiarities and metallothionein content in human thyroid gland under iodine deficiency euthyroid nodular goiter |
title_fullStr |
Trace elements storage peculiarities and metallothionein content in human thyroid gland under iodine deficiency euthyroid nodular goiter |
title_full_unstemmed |
Trace elements storage peculiarities and metallothionein content in human thyroid gland under iodine deficiency euthyroid nodular goiter |
title_sort |
trace elements storage peculiarities and metallothionein content in human thyroid gland under iodine deficiency euthyroid nodular goiter |
publisher |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. |
series |
Ukrainian Biochemical Journal |
issn |
2409-4943 2413-5003 |
publishDate |
2014-06-01 |
description |
Accumulation of iodine and copper in the node, paranodular and contralateral (not affected tissue by node) tissues of thyroid gland in relation to the level of metal-binding proteins, potential antioxidants and oxidative changes in tissue was investigated. To assess the severity of the pathological process the molecular markers of cytotoxicity were used. The reduction of total iodine (by 19.5%), increase of inorganic iodine fraction (by 82.4%) and total copper content (twice) in paranodular and nodular tissues compared with contrlateral part have been established. Excess of copper in goitrous-changes tissue was partially accumulated in the metallothioneins. The level of metal-binding form of metallothioneins and reserve of free thiols of these proteins was higher two-three times and lower content of reduced glutathione in node-affected tissue compared to the contralateral part. Signs of cytotoxicity among them: higher cathepsine D free activity (up to 84.6% and 134.4% in paranodular tissue and node respectively) and higher level of DNA strand breaks in the node (up to 22.6%) were observed. In paranodular tissue the range of indices variability compared with parenchyma of contralateral part is shorter than in the node. Thus, under low level of iodine organification and high copper level in goitrous-modified tissue of thyroid gland metallothionein may provide a partial compensatory effect on prooxidative processes. |
topic |
apoptosis copper cytotoxicity iodine iodine deficiency nodular colloidal goiter metallothioneins zinc |
url |
http://ukrbiochemjournal.org/wp-content/uploads/2015/06/Falfushynska_3_14.pdf |
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