Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern Brazil
Abstract Oriental fruit moth (Grapholita molesta) is the most important pest of apple orchards in Brazil. For many years, insecticides have been the only tool used for its control. The aim of this work was to characterize the management of apple orchards by the system for Assessment of Environment I...
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Sociedade Brasileira de Fruticultura
2018-07-01
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doaj-6775077f9d08413e9cacb2b06f0332882020-11-25T00:43:21ZengSociedade Brasileira de FruticulturaRevista Brasileira de Fruticultura1806-99672018-07-0140410.1590/0100-29452018608S0100-29452018000400402Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern BrazilLino Bittencourt MonteiroRosangela TeixeiraIzonete Cristina GuiloskiEdson ChappuisHelena Cristina da Silva de AssisAbstract Oriental fruit moth (Grapholita molesta) is the most important pest of apple orchards in Brazil. For many years, insecticides have been the only tool used for its control. The aim of this work was to characterize the management of apple orchards by the system for Assessment of Environment Impact of Agricultural Technology Innovations (AMBITEC-AGRO) and to relate it with the resistance status of G. molesta populations. Insecticide resistance was estimated by toxicology and enzymatic activities of glutathione S-transferase (GST), esterases (a-ß EST), and acetylcholinesterase (AChE). The phytosanitary strategies adopted by the four apple production orchards in southern Brazil were analyzed in: Vacaria (RS population), Fraiburgo (SC1 population), São Joaquim (SC2 population), and Porto Amazonas (PR population). Oriental fruit moth field populations were reared in laboratory for bioassays with chlorpyriphos, carbaryl, deltamethrin, and tebufenozide insecticides and were compared with a reference susceptible population. Larval mortality rates were recorded for seven days. GST, a-ß EST, and AChE assays were performed with third- and fourth-instar larvae. Results indicated that oriental fruit moth populations collected in Vacaria, Fraiburgo, and São Joaquim showed tolerance to carbaryl and chlorpyriphos, whereas the population collected in Porto Amazonas was more susceptible. SC2 and PR populations were more tolerant to deltamethrin, while RS and SC1 populations were more susceptible. No population was tolerant to tebufenozide. RS population showed the highest activity for AChE, GST, and a-EST. The findings of this study suggest that the AMBITEC-AGRO system was a suitable method to evaluate the phytosanitary activity of apple orchards and to relate it to the resistance status of G. molesta populations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000400402&lng=en&tlng=enResistance managementGrapholita molestasusceptibilityapple orchard |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lino Bittencourt Monteiro Rosangela Teixeira Izonete Cristina Guiloski Edson Chappuis Helena Cristina da Silva de Assis |
spellingShingle |
Lino Bittencourt Monteiro Rosangela Teixeira Izonete Cristina Guiloski Edson Chappuis Helena Cristina da Silva de Assis Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern Brazil Revista Brasileira de Fruticultura Resistance management Grapholita molesta susceptibility apple orchard |
author_facet |
Lino Bittencourt Monteiro Rosangela Teixeira Izonete Cristina Guiloski Edson Chappuis Helena Cristina da Silva de Assis |
author_sort |
Lino Bittencourt Monteiro |
title |
Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern Brazil |
title_short |
Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern Brazil |
title_full |
Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern Brazil |
title_fullStr |
Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern Brazil |
title_full_unstemmed |
Resistance of oriental fruit moth (Lepidoptera: tortricidae) to insecticides in apple orchards in southern Brazil |
title_sort |
resistance of oriental fruit moth (lepidoptera: tortricidae) to insecticides in apple orchards in southern brazil |
publisher |
Sociedade Brasileira de Fruticultura |
series |
Revista Brasileira de Fruticultura |
issn |
1806-9967 |
publishDate |
2018-07-01 |
description |
Abstract Oriental fruit moth (Grapholita molesta) is the most important pest of apple orchards in Brazil. For many years, insecticides have been the only tool used for its control. The aim of this work was to characterize the management of apple orchards by the system for Assessment of Environment Impact of Agricultural Technology Innovations (AMBITEC-AGRO) and to relate it with the resistance status of G. molesta populations. Insecticide resistance was estimated by toxicology and enzymatic activities of glutathione S-transferase (GST), esterases (a-ß EST), and acetylcholinesterase (AChE). The phytosanitary strategies adopted by the four apple production orchards in southern Brazil were analyzed in: Vacaria (RS population), Fraiburgo (SC1 population), São Joaquim (SC2 population), and Porto Amazonas (PR population). Oriental fruit moth field populations were reared in laboratory for bioassays with chlorpyriphos, carbaryl, deltamethrin, and tebufenozide insecticides and were compared with a reference susceptible population. Larval mortality rates were recorded for seven days. GST, a-ß EST, and AChE assays were performed with third- and fourth-instar larvae. Results indicated that oriental fruit moth populations collected in Vacaria, Fraiburgo, and São Joaquim showed tolerance to carbaryl and chlorpyriphos, whereas the population collected in Porto Amazonas was more susceptible. SC2 and PR populations were more tolerant to deltamethrin, while RS and SC1 populations were more susceptible. No population was tolerant to tebufenozide. RS population showed the highest activity for AChE, GST, and a-EST. The findings of this study suggest that the AMBITEC-AGRO system was a suitable method to evaluate the phytosanitary activity of apple orchards and to relate it to the resistance status of G. molesta populations. |
topic |
Resistance management Grapholita molesta susceptibility apple orchard |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000400402&lng=en&tlng=en |
work_keys_str_mv |
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