Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience
Neuronal activity is dominated by synaptic inputs from excitatory or inhibitory neural circuits. With the development of in vivo patch-clamp recording, especially in vivo voltage-clamp recording, researchers can not only directly measure neuronal activity, such as spiking responses or membrane poten...
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Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncir.2015.00023/full |
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doaj-677a54440f3348d3a3c7f0ad331e40bc2020-11-24T20:51:24ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102015-05-01910.3389/fncir.2015.00023141426Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscienceCan eTao0Guangwei eZhang1Ying eXiong2Yi eZhou3Third Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityThird Military Medical UniversityNeuronal activity is dominated by synaptic inputs from excitatory or inhibitory neural circuits. With the development of in vivo patch-clamp recording, especially in vivo voltage-clamp recording, researchers can not only directly measure neuronal activity, such as spiking responses or membrane potential dynamics, but also quantify synaptic inputs from excitatory and inhibitory circuits in living animals. This approach enables researchers to directly unravel different synaptic components and to understand their underlying roles in particular brain functions. Combining in vivo patch-clamp recording with other techniques, such as two-photon imaging or optogenetics, can provide even clearer functional dissection of the synaptic contributions of different neurons or nuclei. Here, we summarized current applications and recent research progress using the in vivo patch-clamp recording method and focused on its role in the functional dissection of different synaptic inputs. The key factors of a successful in vivo patch-clamp experiment and possible solutions based on references and our experiences were also discussed.http://journal.frontiersin.org/Journal/10.3389/fncir.2015.00023/fullneural circuitsin vivopatch-clampsensory cortexsynaptic contribution |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Can eTao Guangwei eZhang Ying eXiong Yi eZhou |
spellingShingle |
Can eTao Guangwei eZhang Ying eXiong Yi eZhou Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience Frontiers in Neural Circuits neural circuits in vivo patch-clamp sensory cortex synaptic contribution |
author_facet |
Can eTao Guangwei eZhang Ying eXiong Yi eZhou |
author_sort |
Can eTao |
title |
Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience |
title_short |
Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience |
title_full |
Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience |
title_fullStr |
Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience |
title_full_unstemmed |
Functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience |
title_sort |
functional dissection of synaptic circuits: in vivo patch-clamp recording in neuroscience |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neural Circuits |
issn |
1662-5110 |
publishDate |
2015-05-01 |
description |
Neuronal activity is dominated by synaptic inputs from excitatory or inhibitory neural circuits. With the development of in vivo patch-clamp recording, especially in vivo voltage-clamp recording, researchers can not only directly measure neuronal activity, such as spiking responses or membrane potential dynamics, but also quantify synaptic inputs from excitatory and inhibitory circuits in living animals. This approach enables researchers to directly unravel different synaptic components and to understand their underlying roles in particular brain functions. Combining in vivo patch-clamp recording with other techniques, such as two-photon imaging or optogenetics, can provide even clearer functional dissection of the synaptic contributions of different neurons or nuclei. Here, we summarized current applications and recent research progress using the in vivo patch-clamp recording method and focused on its role in the functional dissection of different synaptic inputs. The key factors of a successful in vivo patch-clamp experiment and possible solutions based on references and our experiences were also discussed. |
topic |
neural circuits in vivo patch-clamp sensory cortex synaptic contribution |
url |
http://journal.frontiersin.org/Journal/10.3389/fncir.2015.00023/full |
work_keys_str_mv |
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1716802324049952768 |