Andrographolide from the herb Andrographis paniculata induces apoptosis on cultured human leukemic cells
Objectives: Andrographis paniculata (A. paniculata) is a widely used herb that has potential medical properties. Andrographolide (Andro) is the major component of A. paniculata. We evaluated the anti-tumor activity of Andro using leukemic cell line cells. Methods: Leukemic cell lines U937, HL60 or H...
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Fujita Medical Society
2017-09-01
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doaj-678fef0369a54ebfae24a15f6e2d15bb2020-11-25T00:58:57ZengFujita Medical SocietyFujita Medical Journal2189-72472189-72552017-09-0133485410.20407/fmj.3.3_48Andrographolide from the herb Andrographis paniculata induces apoptosis on cultured human leukemic cellsHiroki DoiTaei MatsuiTamae OhyeKuniaki SaitoItsuro KatsudaHidehiko AkiyamaObjectives: Andrographis paniculata (A. paniculata) is a widely used herb that has potential medical properties. Andrographolide (Andro) is the major component of A. paniculata. We evaluated the anti-tumor activity of Andro using leukemic cell line cells. Methods: Leukemic cell lines U937, HL60 or H929 cells were cultured in the presence or absence of Andro and compared with the effects of Ara-C or vincristine. The anti-tumor activity was assessed by morphological observations of the cells, DNA fragmentation, MTT assay, Annexin V positive rate, caspase-3/7 activity, and cell cycle analysis. Results: After addition of Andro, the morphology of cells changed to characteristic shapes with apoptotic bodies. Furthermore, the Annexin V positive rate and caspase-3/7 activities were increased compared with untreated cells. The G1 phase of cell cycle was also similarly increased compared with cells treated with Ara-C. Conclusions: Our results show that Andro has an anti-tumor activity against leukemic cell lines, very possibly by inducing apoptosis.https://www.jstage.jst.go.jp/article/fmj/3/3/3_2016023/_pdf/-char/enandrographis paniculataandrographolideapoptosisleukemic cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hiroki Doi Taei Matsui Tamae Ohye Kuniaki Saito Itsuro Katsuda Hidehiko Akiyama |
spellingShingle |
Hiroki Doi Taei Matsui Tamae Ohye Kuniaki Saito Itsuro Katsuda Hidehiko Akiyama Andrographolide from the herb Andrographis paniculata induces apoptosis on cultured human leukemic cells Fujita Medical Journal andrographis paniculata andrographolide apoptosis leukemic cells |
author_facet |
Hiroki Doi Taei Matsui Tamae Ohye Kuniaki Saito Itsuro Katsuda Hidehiko Akiyama |
author_sort |
Hiroki Doi |
title |
Andrographolide from the herb Andrographis paniculata induces apoptosis on cultured human leukemic cells |
title_short |
Andrographolide from the herb Andrographis paniculata induces apoptosis on cultured human leukemic cells |
title_full |
Andrographolide from the herb Andrographis paniculata induces apoptosis on cultured human leukemic cells |
title_fullStr |
Andrographolide from the herb Andrographis paniculata induces apoptosis on cultured human leukemic cells |
title_full_unstemmed |
Andrographolide from the herb Andrographis paniculata induces apoptosis on cultured human leukemic cells |
title_sort |
andrographolide from the herb andrographis paniculata induces apoptosis on cultured human leukemic cells |
publisher |
Fujita Medical Society |
series |
Fujita Medical Journal |
issn |
2189-7247 2189-7255 |
publishDate |
2017-09-01 |
description |
Objectives: Andrographis paniculata (A. paniculata) is a widely used herb that has potential medical properties. Andrographolide (Andro) is the major component of A. paniculata. We evaluated the anti-tumor activity of Andro using leukemic cell line cells. Methods: Leukemic cell lines U937, HL60 or H929 cells were cultured in the presence or absence of Andro and compared with the effects of Ara-C or vincristine. The anti-tumor activity was assessed by morphological observations of the cells, DNA fragmentation, MTT assay, Annexin V positive rate, caspase-3/7 activity, and cell cycle analysis. Results: After addition of Andro, the morphology of cells changed to characteristic shapes with apoptotic bodies. Furthermore, the Annexin V positive rate and caspase-3/7 activities were increased compared with untreated cells. The G1 phase of cell cycle was also similarly increased compared with cells treated with Ara-C. Conclusions: Our results show that Andro has an anti-tumor activity against leukemic cell lines, very possibly by inducing apoptosis. |
topic |
andrographis paniculata andrographolide apoptosis leukemic cells |
url |
https://www.jstage.jst.go.jp/article/fmj/3/3/3_2016023/_pdf/-char/en |
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