LEMD1-AS1 Suppresses Ovarian Cancer Progression Through Regulating miR-183-5p/TP53 Axis
Ruowen Guo, Yide Qin Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, 230032, People’s Republic of ChinaCorrespondence: Yide Qin Email yideeduca@126.comBackground/Aims: Long noncoding RNAs (lncRNAs) play a critical...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Dove Medical Press
2020-07-01
|
Series: | OncoTargets and Therapy |
Subjects: | |
Online Access: | https://www.dovepress.com/lemd1-as1-suppresses-ovarian-cancer-progression-through-regulating-mir-peer-reviewed-article-OTT |
id |
doaj-679810460a44422e9650eb7e88b33978 |
---|---|
record_format |
Article |
spelling |
doaj-679810460a44422e9650eb7e88b339782020-11-25T03:01:46ZengDove Medical PressOncoTargets and Therapy1178-69302020-07-01Volume 137387739855723LEMD1-AS1 Suppresses Ovarian Cancer Progression Through Regulating miR-183-5p/TP53 AxisGuo RQin YRuowen Guo, Yide Qin Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, 230032, People’s Republic of ChinaCorrespondence: Yide Qin Email yideeduca@126.comBackground/Aims: Long noncoding RNAs (lncRNAs) play a critical role in tumorigenesis and progression of ovarian cancer (OC). This study focused on the function and potential mechanism toward LEMD1-AS1 (LEMD1 antisense RNA 1) in the progression of ovarian cancer.Materials and Methods: The expression of LEMD1-AS1 in OC tissues was evaluated in TCGA and Gene Expression Omnibus datasets (GSE119056) and confirmed in OC cell lines via qRT-PCR (quantitative real-time polymerase chain reaction). Then, the location of LEMD1-AS1 in the cytoplasmic and nuclear RNAs extracted from OV cells was detected by qRT-PCR. Cell Counting Kit-8 (CCK-8), colony formation, wound-healing and transwell assays were applied to examine cell viability, proliferation, migration and invasion, respectively. Further, the effect of LEMD1-AS1 on OC tumor growth was determined via subcutaneous xenotransplanted tumor model. The potential target for LEMD1-AS1 was validated via dual-luciferase activity assay, RNA pull-down and RNA immunoprecipitation.Results: The expression of LEMD1-AS1 was decreased in OC tissues and cell lines. Forced overexpression of LEMD1-AS1 inhibited the proliferation, migration and invasion of ovarian cancer cells and transplanted tumor growth in nude mice. We found that LEMD1-AS1 was mainly located in the cytoplasm of OC cells and contained complementary sites of miR-183-5p. Mechanistically, our results showed that LEMD1-AS1 could directly interact with miR-183-5p and tumor protein p53 (TP53). The anti-tumor role of LEMD1-AS1 on OC progression depended on miR-183-5p-mediated TP53 expression.Conclusion: LEMD1-AS1 suppresses OC progression through sponging miR-183-5p and regulation of TP53, suggesting a novel biomarker and target for OC.Keywords: LEMD1-AS1, long noncoding RNA, ovarian cancer, tumor progression, miR-183-5p, TP53https://www.dovepress.com/lemd1-as1-suppresses-ovarian-cancer-progression-through-regulating-mir-peer-reviewed-article-OTTlemd1-as1long noncoding rnaovarian cancertumor progressionmir-183-5ptp53 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guo R Qin Y |
spellingShingle |
Guo R Qin Y LEMD1-AS1 Suppresses Ovarian Cancer Progression Through Regulating miR-183-5p/TP53 Axis OncoTargets and Therapy lemd1-as1 long noncoding rna ovarian cancer tumor progression mir-183-5p tp53 |
author_facet |
Guo R Qin Y |
author_sort |
Guo R |
title |
LEMD1-AS1 Suppresses Ovarian Cancer Progression Through Regulating miR-183-5p/TP53 Axis |
title_short |
LEMD1-AS1 Suppresses Ovarian Cancer Progression Through Regulating miR-183-5p/TP53 Axis |
title_full |
LEMD1-AS1 Suppresses Ovarian Cancer Progression Through Regulating miR-183-5p/TP53 Axis |
title_fullStr |
LEMD1-AS1 Suppresses Ovarian Cancer Progression Through Regulating miR-183-5p/TP53 Axis |
title_full_unstemmed |
LEMD1-AS1 Suppresses Ovarian Cancer Progression Through Regulating miR-183-5p/TP53 Axis |
title_sort |
lemd1-as1 suppresses ovarian cancer progression through regulating mir-183-5p/tp53 axis |
publisher |
Dove Medical Press |
series |
OncoTargets and Therapy |
issn |
1178-6930 |
publishDate |
2020-07-01 |
description |
Ruowen Guo, Yide Qin Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, 230032, People’s Republic of ChinaCorrespondence: Yide Qin Email yideeduca@126.comBackground/Aims: Long noncoding RNAs (lncRNAs) play a critical role in tumorigenesis and progression of ovarian cancer (OC). This study focused on the function and potential mechanism toward LEMD1-AS1 (LEMD1 antisense RNA 1) in the progression of ovarian cancer.Materials and Methods: The expression of LEMD1-AS1 in OC tissues was evaluated in TCGA and Gene Expression Omnibus datasets (GSE119056) and confirmed in OC cell lines via qRT-PCR (quantitative real-time polymerase chain reaction). Then, the location of LEMD1-AS1 in the cytoplasmic and nuclear RNAs extracted from OV cells was detected by qRT-PCR. Cell Counting Kit-8 (CCK-8), colony formation, wound-healing and transwell assays were applied to examine cell viability, proliferation, migration and invasion, respectively. Further, the effect of LEMD1-AS1 on OC tumor growth was determined via subcutaneous xenotransplanted tumor model. The potential target for LEMD1-AS1 was validated via dual-luciferase activity assay, RNA pull-down and RNA immunoprecipitation.Results: The expression of LEMD1-AS1 was decreased in OC tissues and cell lines. Forced overexpression of LEMD1-AS1 inhibited the proliferation, migration and invasion of ovarian cancer cells and transplanted tumor growth in nude mice. We found that LEMD1-AS1 was mainly located in the cytoplasm of OC cells and contained complementary sites of miR-183-5p. Mechanistically, our results showed that LEMD1-AS1 could directly interact with miR-183-5p and tumor protein p53 (TP53). The anti-tumor role of LEMD1-AS1 on OC progression depended on miR-183-5p-mediated TP53 expression.Conclusion: LEMD1-AS1 suppresses OC progression through sponging miR-183-5p and regulation of TP53, suggesting a novel biomarker and target for OC.Keywords: LEMD1-AS1, long noncoding RNA, ovarian cancer, tumor progression, miR-183-5p, TP53 |
topic |
lemd1-as1 long noncoding rna ovarian cancer tumor progression mir-183-5p tp53 |
url |
https://www.dovepress.com/lemd1-as1-suppresses-ovarian-cancer-progression-through-regulating-mir-peer-reviewed-article-OTT |
work_keys_str_mv |
AT guor lemd1as1suppressesovariancancerprogressionthroughregulatingmir1835ptp53axis AT qiny lemd1as1suppressesovariancancerprogressionthroughregulatingmir1835ptp53axis |
_version_ |
1724692077522976768 |