SIRT1 Regulates the Human Alveolar Epithelial A549 Cell Apoptosis Induced by Pseudomonas Aeruginosa Lipopolysaccharide
Background: Sirtuin1 (SIRT1) is an NAD+-dependent deacetylase that plays an inhibitory role in cell apoptosis, which is associated with p53 deacetylation. Lipopolysaccharide (LPS) is a key virulence factor produced by Pseudomonas aeruginosa and plays an important role in mediating the interactions b...
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Cell Physiol Biochem Press GmbH & Co KG
2013-01-01
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doaj-6798df2b8b104e6f8cd624d425a9913a2020-11-24T21:26:06ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-01-013119210110.1159/000343352343352SIRT1 Regulates the Human Alveolar Epithelial A549 Cell Apoptosis Induced by Pseudomonas Aeruginosa LipopolysaccharideXiaoli LiuKuiqing ShaoTieying SunBackground: Sirtuin1 (SIRT1) is an NAD+-dependent deacetylase that plays an inhibitory role in cell apoptosis, which is associated with p53 deacetylation. Lipopolysaccharide (LPS) is a key virulence factor produced by Pseudomonas aeruginosa and plays an important role in mediating the interactions between the bacterium and its host. However, the effect of SIRT1 in the regulation of LPS-induced human alveolar epithelial A549 cells apoptosis is unknown. Methods: Cell viability, apoptosis and reactive oxygen species (ROS) production were first examined in A549 cells that were treated with LPS. Relative cell signaling pathways were further explored by western blot analysis. Results: Exposure of A549 cells to LPS decreased cell viability in a concentration- and time- dependent manner. LPS stimulated cell apoptosis and ROS production while inhibiting the expression of SIRT1 in A549 cells. Activation of SIRT1 by exposure to resveratrol significantly reversed the effects of LPS on A549 cells. In contrast, inhibition of SIRT1 by nicotinamide had the opposite effects enhancing cell apoptosis and ROS production. Conclusion: SIRT1 plays an important role in regulating the human alveolar epithelial A549 cell apoptosis process induced by LPS.http://www.karger.com/Article/FullText/343352LPSSIRT1ApoptosisROS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoli Liu Kuiqing Shao Tieying Sun |
spellingShingle |
Xiaoli Liu Kuiqing Shao Tieying Sun SIRT1 Regulates the Human Alveolar Epithelial A549 Cell Apoptosis Induced by Pseudomonas Aeruginosa Lipopolysaccharide Cellular Physiology and Biochemistry LPS SIRT1 Apoptosis ROS |
author_facet |
Xiaoli Liu Kuiqing Shao Tieying Sun |
author_sort |
Xiaoli Liu |
title |
SIRT1 Regulates the Human Alveolar Epithelial A549 Cell Apoptosis Induced by Pseudomonas Aeruginosa Lipopolysaccharide |
title_short |
SIRT1 Regulates the Human Alveolar Epithelial A549 Cell Apoptosis Induced by Pseudomonas Aeruginosa Lipopolysaccharide |
title_full |
SIRT1 Regulates the Human Alveolar Epithelial A549 Cell Apoptosis Induced by Pseudomonas Aeruginosa Lipopolysaccharide |
title_fullStr |
SIRT1 Regulates the Human Alveolar Epithelial A549 Cell Apoptosis Induced by Pseudomonas Aeruginosa Lipopolysaccharide |
title_full_unstemmed |
SIRT1 Regulates the Human Alveolar Epithelial A549 Cell Apoptosis Induced by Pseudomonas Aeruginosa Lipopolysaccharide |
title_sort |
sirt1 regulates the human alveolar epithelial a549 cell apoptosis induced by pseudomonas aeruginosa lipopolysaccharide |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2013-01-01 |
description |
Background: Sirtuin1 (SIRT1) is an NAD+-dependent deacetylase that plays an inhibitory role in cell apoptosis, which is associated with p53 deacetylation. Lipopolysaccharide (LPS) is a key virulence factor produced by Pseudomonas aeruginosa and plays an important role in mediating the interactions between the bacterium and its host. However, the effect of SIRT1 in the regulation of LPS-induced human alveolar epithelial A549 cells apoptosis is unknown. Methods: Cell viability, apoptosis and reactive oxygen species (ROS) production were first examined in A549 cells that were treated with LPS. Relative cell signaling pathways were further explored by western blot analysis. Results: Exposure of A549 cells to LPS decreased cell viability in a concentration- and time- dependent manner. LPS stimulated cell apoptosis and ROS production while inhibiting the expression of SIRT1 in A549 cells. Activation of SIRT1 by exposure to resveratrol significantly reversed the effects of LPS on A549 cells. In contrast, inhibition of SIRT1 by nicotinamide had the opposite effects enhancing cell apoptosis and ROS production. Conclusion: SIRT1 plays an important role in regulating the human alveolar epithelial A549 cell apoptosis process induced by LPS. |
topic |
LPS SIRT1 Apoptosis ROS |
url |
http://www.karger.com/Article/FullText/343352 |
work_keys_str_mv |
AT xiaoliliu sirt1regulatesthehumanalveolarepitheliala549cellapoptosisinducedbypseudomonasaeruginosalipopolysaccharide AT kuiqingshao sirt1regulatesthehumanalveolarepitheliala549cellapoptosisinducedbypseudomonasaeruginosalipopolysaccharide AT tieyingsun sirt1regulatesthehumanalveolarepitheliala549cellapoptosisinducedbypseudomonasaeruginosalipopolysaccharide |
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1725980940168069120 |