Comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model.
Intracavernous pressure (ICP) measurement is a well-established technique for assessing the erectile function, which was performed by cannulating either crus or shaft of the penis. However, there are no studies concerning the experimental performance of the two cannulation sites yet. The aim of this...
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doaj-679ce12e70e641dda5cf753b4ab0c6a72020-11-25T02:47:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01132e019354310.1371/journal.pone.0193543Comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model.Shankun ZhaoRan KangTuo DengLianmin LuoJiamin WangErmao LiJintai LuoLuhao LiuShawPong WanZhigang ZhaoIntracavernous pressure (ICP) measurement is a well-established technique for assessing the erectile function, which was performed by cannulating either crus or shaft of the penis. However, there are no studies concerning the experimental performance of the two cannulation sites yet. The aim of this study was to compare the measuring outcomes using two different cannulation sites. To validate the capacity of our study, both normal and the castration-induced erectile dysfunction rat models were conducted. Fifty adult male Sprague-Dawley rats were randomized equally into two groups: an intact group and a castration group. Five rats from each group firstly underwent different stimulation parameters to detect the optimal erectile responses. The residual rats in each group were further assigned into two subgroups (n = 10 per subgroup) according to two different cannulation sites (crus or shaft of the corpus cavernosum). The ICP values were compared between groups after different interventions. The optimal parameters for mean maximum ICP were recorded at 2.5V and a frequency of 15 Hz. The rats under the two different cannulation sites tended to show similar ICP values in both the intact and the castration groups. However, the success rate in monitoring ICP was significantly higher in the groups cannulating into the shaft of the penis compared to the crus (100% vs. 70%; P = 0.02). Our data suggested that the method of cannulation into the penile shaft could serve as a better alternative for the ICP measurement in rats.http://europepmc.org/articles/PMC5828359?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shankun Zhao Ran Kang Tuo Deng Lianmin Luo Jiamin Wang Ermao Li Jintai Luo Luhao Liu ShawPong Wan Zhigang Zhao |
spellingShingle |
Shankun Zhao Ran Kang Tuo Deng Lianmin Luo Jiamin Wang Ermao Li Jintai Luo Luhao Liu ShawPong Wan Zhigang Zhao Comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model. PLoS ONE |
author_facet |
Shankun Zhao Ran Kang Tuo Deng Lianmin Luo Jiamin Wang Ermao Li Jintai Luo Luhao Liu ShawPong Wan Zhigang Zhao |
author_sort |
Shankun Zhao |
title |
Comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model. |
title_short |
Comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model. |
title_full |
Comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model. |
title_fullStr |
Comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model. |
title_full_unstemmed |
Comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model. |
title_sort |
comparison of two cannulation methods for assessment of intracavernosal pressure in a rat model. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2018-01-01 |
description |
Intracavernous pressure (ICP) measurement is a well-established technique for assessing the erectile function, which was performed by cannulating either crus or shaft of the penis. However, there are no studies concerning the experimental performance of the two cannulation sites yet. The aim of this study was to compare the measuring outcomes using two different cannulation sites. To validate the capacity of our study, both normal and the castration-induced erectile dysfunction rat models were conducted. Fifty adult male Sprague-Dawley rats were randomized equally into two groups: an intact group and a castration group. Five rats from each group firstly underwent different stimulation parameters to detect the optimal erectile responses. The residual rats in each group were further assigned into two subgroups (n = 10 per subgroup) according to two different cannulation sites (crus or shaft of the corpus cavernosum). The ICP values were compared between groups after different interventions. The optimal parameters for mean maximum ICP were recorded at 2.5V and a frequency of 15 Hz. The rats under the two different cannulation sites tended to show similar ICP values in both the intact and the castration groups. However, the success rate in monitoring ICP was significantly higher in the groups cannulating into the shaft of the penis compared to the crus (100% vs. 70%; P = 0.02). Our data suggested that the method of cannulation into the penile shaft could serve as a better alternative for the ICP measurement in rats. |
url |
http://europepmc.org/articles/PMC5828359?pdf=render |
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