| Summary: | In this study, a Bionectria sp. strain isolated from citrus rhizosphere was evaluated for its potential in inhibiting the growth of
Rhizoctonia solani and Pythium aphanidermatum. It was demonstrated that Bionectria sp. 6.21 inhibited the growth of P. aphanidermatum
and R. solani. In dual cultures, however, the antagonist only parasitised R. solani. Regarding the assay involving P. aphanidermatum,
a lack of mycoparasitic ability was demonstrated. Crude extract of Bionectria completely inhibited the mycelial growth of both fungi.
It appears that the main mechanism involved in the antagonism of Pythium by Bionectria is through antibiotic production. The antagonistic
fungus released extracellular secondary metabolites. The metabolites were found to be inhibitory to both plant pathogenic
fungi. From the crude extract, eleven fractions were obtained and tested for their antifungal properties. Two of them showed very
strong activity against P. aphanidermatum. The obtained results indicated that this biocontrol agent has both antibiotic and mycoparasitic
properties. On the other hand, evidence obtained from Scanning Electron Microscopy (SEM) suggests the involvement of an
enzymatic process, with enzymatic digestion playing a major role in the parasitism of Bionectria sp. 6.21. In conclusion, these results
provide evidence that mainly due to mycoparasitism, this strain has the potential to become a good candidate for biological control.
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