Bacteriophage-Derived Peptidase CHAPK Eliminates and Prevents Staphylococcal Biofilms

Bacteriophage-Derived Peptidase CHAPK Eliminates and Prevents Staphylococcal Biofilms

New antibacterial agents are urgently needed for the elimination of biofilm-forming bacteria that are highly resistant to traditional antimicrobial agents. Proliferation of such bacteria can lead to significant economic losses in the agri-food sector. This study demonstrates the potential of the bac...

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Main Authors: Mark Fenton, Ruth Keary, Olivia McAuliffe, R. Paul Ross, Jim O'Mahony, Aidan Coffey
Format: Article
Language:English
Published: Hindawi Limited 2013-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/2013/625341
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spelling doaj-67a97f8915af4a5794243a9ff2b386472021-07-02T02:29:12ZengHindawi LimitedInternational Journal of Microbiology1687-918X1687-91982013-01-01201310.1155/2013/625341625341Bacteriophage-Derived Peptidase CHAPK Eliminates and Prevents Staphylococcal BiofilmsMark Fenton0Ruth Keary1Olivia McAuliffe2R. Paul Ross3Jim O'Mahony4Aidan Coffey5Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, IrelandDepartment of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, IrelandTeagasc, Moorepark Food Research Centre, Fermoy, County Cork, IrelandTeagasc, Moorepark Food Research Centre, Fermoy, County Cork, IrelandDepartment of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, IrelandDepartment of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, IrelandNew antibacterial agents are urgently needed for the elimination of biofilm-forming bacteria that are highly resistant to traditional antimicrobial agents. Proliferation of such bacteria can lead to significant economic losses in the agri-food sector. This study demonstrates the potential of the bacteriophage-derived peptidase, CHAPK, as a biocidal agent for the rapid disruption of biofilm-forming staphylococci, commonly associated with bovine mastitis. Purified CHAPK applied to biofilms of Staphylococcus aureus DPC5246 completely eliminated the staphylococcal biofilms within 4 h. In addition, CHAPK was able to prevent biofilm formation by this strain. The CHAPK lysin also reduced S. aureus in a skin decolonization model. Our data demonstrates the potential of CHAPK as a biocidal agent for prevention and treatment of biofilm-associated staphylococcal infections or as a decontaminating agent in the food and healthcare sectors.http://dx.doi.org/10.1155/2013/625341
collection DOAJ
language English
format Article
sources DOAJ
author Mark Fenton
Ruth Keary
Olivia McAuliffe
R. Paul Ross
Jim O'Mahony
Aidan Coffey
spellingShingle Mark Fenton
Ruth Keary
Olivia McAuliffe
R. Paul Ross
Jim O'Mahony
Aidan Coffey
Bacteriophage-Derived Peptidase CHAPK Eliminates and Prevents Staphylococcal Biofilms
International Journal of Microbiology
author_facet Mark Fenton
Ruth Keary
Olivia McAuliffe
R. Paul Ross
Jim O'Mahony
Aidan Coffey
author_sort Mark Fenton
title Bacteriophage-Derived Peptidase CHAPK Eliminates and Prevents Staphylococcal Biofilms
title_short Bacteriophage-Derived Peptidase CHAPK Eliminates and Prevents Staphylococcal Biofilms
title_full Bacteriophage-Derived Peptidase CHAPK Eliminates and Prevents Staphylococcal Biofilms
title_fullStr Bacteriophage-Derived Peptidase CHAPK Eliminates and Prevents Staphylococcal Biofilms
title_full_unstemmed Bacteriophage-Derived Peptidase CHAPK Eliminates and Prevents Staphylococcal Biofilms
title_sort bacteriophage-derived peptidase chapk eliminates and prevents staphylococcal biofilms
publisher Hindawi Limited
series International Journal of Microbiology
issn 1687-918X
1687-9198
publishDate 2013-01-01
description New antibacterial agents are urgently needed for the elimination of biofilm-forming bacteria that are highly resistant to traditional antimicrobial agents. Proliferation of such bacteria can lead to significant economic losses in the agri-food sector. This study demonstrates the potential of the bacteriophage-derived peptidase, CHAPK, as a biocidal agent for the rapid disruption of biofilm-forming staphylococci, commonly associated with bovine mastitis. Purified CHAPK applied to biofilms of Staphylococcus aureus DPC5246 completely eliminated the staphylococcal biofilms within 4 h. In addition, CHAPK was able to prevent biofilm formation by this strain. The CHAPK lysin also reduced S. aureus in a skin decolonization model. Our data demonstrates the potential of CHAPK as a biocidal agent for prevention and treatment of biofilm-associated staphylococcal infections or as a decontaminating agent in the food and healthcare sectors.
url http://dx.doi.org/10.1155/2013/625341
work_keys_str_mv AT markfenton bacteriophagederivedpeptidasechapkeliminatesandpreventsstaphylococcalbiofilms
AT ruthkeary bacteriophagederivedpeptidasechapkeliminatesandpreventsstaphylococcalbiofilms
AT oliviamcauliffe bacteriophagederivedpeptidasechapkeliminatesandpreventsstaphylococcalbiofilms
AT rpaulross bacteriophagederivedpeptidasechapkeliminatesandpreventsstaphylococcalbiofilms
AT jimomahony bacteriophagederivedpeptidasechapkeliminatesandpreventsstaphylococcalbiofilms
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