Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In Vitro
In porcine preimplantation embryos, glucose-utilizing pathways are reported active throughout their development. The aim of this study was to test the involvement of glycolysis activity in early stage of porcine embryo development by the addition of iodoacetate, a glycolytic inhibitor, to culture me...
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Bogor Agricultural University
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doaj-67c03a8a3d8346ee9dcc235beb7ec0422020-11-24T22:25:27ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192009-03-01161252910.4308/hjb.16.1.25Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In VitroNI WAYAN KURNIANI KARJA0MOKHAMAD FAHRUDIN1KAZUHIRO KIKUCHI2Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta 55281, IndonesiaDepartment of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor 16680, IndonesiaDepartment of Animal Sciences, Reproductive Biology Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, JapanIn porcine preimplantation embryos, glucose-utilizing pathways are reported active throughout their development. The aim of this study was to test the involvement of glycolysis activity in early stage of porcine embryo development by the addition of iodoacetate, a glycolytic inhibitor, to culture medium. In vitro matured and fertilized oocytes were cultured for the first 2 days in iodoacetate at concentrations of 0, 1, or 2.5 μM. The proportion of viable embryos on day 4 decreased when Ia was added at concentration of 2.5 μM (P < 0.05). The development of cleaved embryos to the blastocyst stage on day 6 was significantly reduced by 1 μM iodoacetate and none of cleaved embryos in Ia-2.5 group develop to the blasyocyst stage (P < 0.05). More than 60% of embryos in Ia-2.5 group were arrested at the two-four-cell stage, and then arrested at the morula stage. These results indicate that glucose plays important roles in supporting the development of early stage of porcine embryos and that the inhibition of glucose metabolism may disrupt the management of energy production, leaving the embryos incompetence to develop.http://www.sciencedirect.com/science/article/pii/S1978301916302339iodoacetateglucoseearly stage embryosin vitro culture |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
NI WAYAN KURNIANI KARJA MOKHAMAD FAHRUDIN KAZUHIRO KIKUCHI |
spellingShingle |
NI WAYAN KURNIANI KARJA MOKHAMAD FAHRUDIN KAZUHIRO KIKUCHI Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In Vitro Hayati Journal of Biosciences iodoacetate glucose early stage embryos in vitro culture |
author_facet |
NI WAYAN KURNIANI KARJA MOKHAMAD FAHRUDIN KAZUHIRO KIKUCHI |
author_sort |
NI WAYAN KURNIANI KARJA |
title |
Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In Vitro |
title_short |
Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In Vitro |
title_full |
Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In Vitro |
title_fullStr |
Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In Vitro |
title_full_unstemmed |
Inhibitory Effect of Iodoacetate on Developmental Competence of Porcine Early Stage Embryos In Vitro |
title_sort |
inhibitory effect of iodoacetate on developmental competence of porcine early stage embryos in vitro |
publisher |
Bogor Agricultural University |
series |
Hayati Journal of Biosciences |
issn |
1978-3019 |
publishDate |
2009-03-01 |
description |
In porcine preimplantation embryos, glucose-utilizing pathways are reported active throughout their development. The aim of this study was to test the involvement of glycolysis activity in early stage of porcine embryo development by the addition of iodoacetate, a glycolytic inhibitor, to culture medium. In vitro matured and fertilized oocytes were cultured for the first 2 days in iodoacetate at concentrations of 0, 1, or 2.5 μM. The proportion of viable embryos on day 4 decreased when Ia was added at concentration of 2.5 μM (P < 0.05). The development of cleaved embryos to the blastocyst stage on day 6 was significantly reduced by 1 μM iodoacetate and none of cleaved embryos in Ia-2.5 group develop to the blasyocyst stage (P < 0.05). More than 60% of embryos in Ia-2.5 group were arrested at the two-four-cell stage, and then arrested at the morula stage. These results indicate that glucose plays important roles in supporting the development of early stage of porcine embryos and that the inhibition of glucose metabolism may disrupt the management of energy production, leaving the embryos incompetence to develop. |
topic |
iodoacetate glucose early stage embryos in vitro culture |
url |
http://www.sciencedirect.com/science/article/pii/S1978301916302339 |
work_keys_str_mv |
AT niwayankurnianikarja inhibitoryeffectofiodoacetateondevelopmentalcompetenceofporcineearlystageembryosinvitro AT mokhamadfahrudin inhibitoryeffectofiodoacetateondevelopmentalcompetenceofporcineearlystageembryosinvitro AT kazuhirokikuchi inhibitoryeffectofiodoacetateondevelopmentalcompetenceofporcineearlystageembryosinvitro |
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