So different and still so similar: The plant compound rosmarinic acid mimics bacterial homoserine lactone quorum sensing signals
Apart from inter-bacteria communication quorum sensing (QS) mechanisms also enable inter-domain interactions. To interfere with bacterial QS, plants were found to secrete compounds; most of which of unknown identity. We have identified the plant compound rosmarinic acid (RA) to modulate Pseudomonas...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2016-03-01
|
Series: | Communicative & Integrative Biology |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/19420889.2016.1156832 |
id |
doaj-67c3a35d099c413da722d6aef9679acf |
---|---|
record_format |
Article |
spelling |
doaj-67c3a35d099c413da722d6aef9679acf2021-02-02T03:20:57ZengTaylor & Francis GroupCommunicative & Integrative Biology1942-08892016-03-019210.1080/19420889.2016.11568321156832So different and still so similar: The plant compound rosmarinic acid mimics bacterial homoserine lactone quorum sensing signalsAndrés Corral-Lugo0Abdelali Daddaoua1Alvaro Ortega2Manuel Espinosa-Urgel3Tino Krell4Estación Experimental del Zaidín, Consejo Superior de Investigaciones CientíficasEstación Experimental del Zaidín, Consejo Superior de Investigaciones CientíficasEstación Experimental del Zaidín, Consejo Superior de Investigaciones CientíficasEstación Experimental del Zaidín, Consejo Superior de Investigaciones CientíficasEstación Experimental del Zaidín, Consejo Superior de Investigaciones CientíficasApart from inter-bacteria communication quorum sensing (QS) mechanisms also enable inter-domain interactions. To interfere with bacterial QS, plants were found to secrete compounds; most of which of unknown identity. We have identified the plant compound rosmarinic acid (RA) to modulate Pseudomonas aeruginosa QS by binding to the RhlR QS regulator. RA was found to be a homoserine-lactone (HSL) mimic that caused agonistic effects on transcription, resulting ultimately in a stimulation of several RhlR controlled phenotypes like virulence factor synthesis or biofilm formation. Our study was initiated by in silico screening of an RhlR model with compound libraries, demonstrating that this approach is suitable to tackle a major bottleneck in signal transduction research, which is the identification of sensor protein ligands. Previous work has shown that plant compounds interfere with the function of orphan QS regulators. Our study demonstrates that this has not necessarily to be the case since RhlR forms a functional pair with the RhlI synthase. A wide range of structurally dissimilar compounds have been found to mimic HSLs suggesting that this class of QS regulators is characterized by a significant plasticity in the recognition of effector molecules. Further research will show to what extent RA impacts on QS mechanisms of other bacteria.http://dx.doi.org/10.1080/19420889.2016.1156832bacterial virulencegene expressioninter-domain signalingplant-bacteria communicationPseudomonas aeruginosaquorum sensingRhlRrosmarinic acidvirulence factors |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andrés Corral-Lugo Abdelali Daddaoua Alvaro Ortega Manuel Espinosa-Urgel Tino Krell |
spellingShingle |
Andrés Corral-Lugo Abdelali Daddaoua Alvaro Ortega Manuel Espinosa-Urgel Tino Krell So different and still so similar: The plant compound rosmarinic acid mimics bacterial homoserine lactone quorum sensing signals Communicative & Integrative Biology bacterial virulence gene expression inter-domain signaling plant-bacteria communication Pseudomonas aeruginosa quorum sensing RhlR rosmarinic acid virulence factors |
author_facet |
Andrés Corral-Lugo Abdelali Daddaoua Alvaro Ortega Manuel Espinosa-Urgel Tino Krell |
author_sort |
Andrés Corral-Lugo |
title |
So different and still so similar: The plant compound rosmarinic acid mimics bacterial homoserine lactone quorum sensing signals |
title_short |
So different and still so similar: The plant compound rosmarinic acid mimics bacterial homoserine lactone quorum sensing signals |
title_full |
So different and still so similar: The plant compound rosmarinic acid mimics bacterial homoserine lactone quorum sensing signals |
title_fullStr |
So different and still so similar: The plant compound rosmarinic acid mimics bacterial homoserine lactone quorum sensing signals |
title_full_unstemmed |
So different and still so similar: The plant compound rosmarinic acid mimics bacterial homoserine lactone quorum sensing signals |
title_sort |
so different and still so similar: the plant compound rosmarinic acid mimics bacterial homoserine lactone quorum sensing signals |
publisher |
Taylor & Francis Group |
series |
Communicative & Integrative Biology |
issn |
1942-0889 |
publishDate |
2016-03-01 |
description |
Apart from inter-bacteria communication quorum sensing (QS) mechanisms also enable inter-domain interactions. To interfere with bacterial QS, plants were found to secrete compounds; most of which of unknown identity. We have identified the plant compound rosmarinic acid (RA) to modulate Pseudomonas aeruginosa QS by binding to the RhlR QS regulator. RA was found to be a homoserine-lactone (HSL) mimic that caused agonistic effects on transcription, resulting ultimately in a stimulation of several RhlR controlled phenotypes like virulence factor synthesis or biofilm formation. Our study was initiated by in silico screening of an RhlR model with compound libraries, demonstrating that this approach is suitable to tackle a major bottleneck in signal transduction research, which is the identification of sensor protein ligands. Previous work has shown that plant compounds interfere with the function of orphan QS regulators. Our study demonstrates that this has not necessarily to be the case since RhlR forms a functional pair with the RhlI synthase. A wide range of structurally dissimilar compounds have been found to mimic HSLs suggesting that this class of QS regulators is characterized by a significant plasticity in the recognition of effector molecules. Further research will show to what extent RA impacts on QS mechanisms of other bacteria. |
topic |
bacterial virulence gene expression inter-domain signaling plant-bacteria communication Pseudomonas aeruginosa quorum sensing RhlR rosmarinic acid virulence factors |
url |
http://dx.doi.org/10.1080/19420889.2016.1156832 |
work_keys_str_mv |
AT andrescorrallugo sodifferentandstillsosimilartheplantcompoundrosmarinicacidmimicsbacterialhomoserinelactonequorumsensingsignals AT abdelalidaddaoua sodifferentandstillsosimilartheplantcompoundrosmarinicacidmimicsbacterialhomoserinelactonequorumsensingsignals AT alvaroortega sodifferentandstillsosimilartheplantcompoundrosmarinicacidmimicsbacterialhomoserinelactonequorumsensingsignals AT manuelespinosaurgel sodifferentandstillsosimilartheplantcompoundrosmarinicacidmimicsbacterialhomoserinelactonequorumsensingsignals AT tinokrell sodifferentandstillsosimilartheplantcompoundrosmarinicacidmimicsbacterialhomoserinelactonequorumsensingsignals |
_version_ |
1724308004828872704 |