Rational Mutagenesis of Cyclodextrin Glucanotransferase at the Calcium Binding Regions for Enhancement of Thermostability
Studies related to the engineering of calcium binding sites of CGTase are limited. The calcium binding regions that are known for thermostability function were subjected to site-directed mutagenesis in this study. The starting gene-protein is a variant of CGTase <em>Bacillus</em...
Main Authors: | Kian Mau Goh, Rosli Md. Illias, Poh Hong Goh |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2012-04-01
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Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | http://www.mdpi.com/1422-0067/13/5/5307 |
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