Rational Mutagenesis of Cyclodextrin Glucanotransferase at the Calcium Binding Regions for Enhancement of Thermostability

Rational Mutagenesis of Cyclodextrin Glucanotransferase at the Calcium Binding Regions for Enhancement of Thermostability

Studies related to the engineering of calcium binding sites of CGTase are limited. The calcium binding regions that are known for thermostability function were subjected to site-directed mutagenesis in this study. The starting gene-protein is a variant of CGTase <em>Bacillus</em...

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Bibliographic Details
Main Authors:	Kian Mau Goh, Rosli Md. Illias, Poh Hong Goh
Format:	Article
Language:	English
Published:	MDPI AG 2012-04-01
Series:	International Journal of Molecular Sciences
Subjects:	CGTase thermostable enzyme site-directed mutagenesis protein engineering calcium binding site
Online Access:	http://www.mdpi.com/1422-0067/13/5/5307

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