Extracellular Production, Characterization, and Engineering of a Polyextremotolerant Subtilisin-Like Protease From Feather-Degrading Thermoactinomyces vulgaris Strain CDF

Extracellular Production, Characterization, and Engineering of a Polyextremotolerant Subtilisin-Like Protease From Feather-Degrading Thermoactinomyces vulgaris Strain CDF

Here, the gene encoding a subtilisin-like protease (protease Als) was cloned from Thermoactinomyces vulgaris strain CDF and expressed in Escherichia coli. The recombinant enzyme was released into the culture medium of E. coli as a mature form (mAls). Purified mAls displayed optimal activity at 60–70...

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Bibliographic Details
Main Authors: Yidi Ding, Yong Yang, Yuxia Ren, Jingying Xia, Feng Liu, Yu Li, Xiao-Feng Tang, Bing Tang
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Microbiology
Subjects:
serine protease
thermostability
halotolerance
organic solvent
detergent
keratinase
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2020.605771/full

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