Sablefish (Anoplopoma fimbra Pallas, 1814) plasma biochemistry and hematology reference intervals including blood cell morphology.
Plasma biochemistry and hematology reference intervals are integral health assessment tools in all medical fields, including aquatic animal health. As sablefish (Anoplopoma fimbria) are becoming aquaculturally and economically more important, this manuscript provides essential reference intervals (R...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2021-01-01
|
Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0246982 |
id |
doaj-67dcd7e13d72478e8f4fb5e19f0d0747 |
---|---|
record_format |
Article |
spelling |
doaj-67dcd7e13d72478e8f4fb5e19f0d07472021-06-10T04:32:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01166e024698210.1371/journal.pone.0246982Sablefish (Anoplopoma fimbra Pallas, 1814) plasma biochemistry and hematology reference intervals including blood cell morphology.Carla B SchubigerM Elena GormanJennifer L JohnsMary R ArkooshJoseph P DietrichPlasma biochemistry and hematology reference intervals are integral health assessment tools in all medical fields, including aquatic animal health. As sablefish (Anoplopoma fimbria) are becoming aquaculturally and economically more important, this manuscript provides essential reference intervals (RI) for their plasma biochemistry and hematology along with reference photomicrographs of blood cells in healthy, fasted sablefish. Blood cell morphology can differ between fish species. In addition, blood cell counts and blood chemistry can vary between fish species, demographics, water conditions, seasons, diets, and culture systems, which precludes the use of RI's from other fish species. For this study, blood was collected for plasma biochemistry and hematology analysis between June 20 and July 18, 2019, from healthy, yearling sablefish, hatched and reared in captivity on a commercial diet. Overnight fast of 16-18 hours did not sufficiently reduce lipids in the blood, which led to visible lipemia and frequent rupture of blood cells during analysis. Therefore, sablefish should be fasted for 24 to 36 hours before blood is collected to reduce hematology artifacts or possible reagent interference in plasma biochemistry analysis. Lymphocytes were the most dominant leukocytes (98%), while eosinophils were rare, and basophils were not detected in sablefish. Neutrophils were very large cells with Döhle bodies. In mammals and avian species, Döhle bodies are usually signs of toxic change from inflammation, but no such association was found in these fish. In conclusion, lipemia can interfere with sablefish blood analysis, and available removal methods should be evaluated as fasting for up to 36 h might not always be feasible. Also, more studies are required to establish RI for different developmental stages and rearing conditions.https://doi.org/10.1371/journal.pone.0246982 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carla B Schubiger M Elena Gorman Jennifer L Johns Mary R Arkoosh Joseph P Dietrich |
spellingShingle |
Carla B Schubiger M Elena Gorman Jennifer L Johns Mary R Arkoosh Joseph P Dietrich Sablefish (Anoplopoma fimbra Pallas, 1814) plasma biochemistry and hematology reference intervals including blood cell morphology. PLoS ONE |
author_facet |
Carla B Schubiger M Elena Gorman Jennifer L Johns Mary R Arkoosh Joseph P Dietrich |
author_sort |
Carla B Schubiger |
title |
Sablefish (Anoplopoma fimbra Pallas, 1814) plasma biochemistry and hematology reference intervals including blood cell morphology. |
title_short |
Sablefish (Anoplopoma fimbra Pallas, 1814) plasma biochemistry and hematology reference intervals including blood cell morphology. |
title_full |
Sablefish (Anoplopoma fimbra Pallas, 1814) plasma biochemistry and hematology reference intervals including blood cell morphology. |
title_fullStr |
Sablefish (Anoplopoma fimbra Pallas, 1814) plasma biochemistry and hematology reference intervals including blood cell morphology. |
title_full_unstemmed |
Sablefish (Anoplopoma fimbra Pallas, 1814) plasma biochemistry and hematology reference intervals including blood cell morphology. |
title_sort |
sablefish (anoplopoma fimbra pallas, 1814) plasma biochemistry and hematology reference intervals including blood cell morphology. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2021-01-01 |
description |
Plasma biochemistry and hematology reference intervals are integral health assessment tools in all medical fields, including aquatic animal health. As sablefish (Anoplopoma fimbria) are becoming aquaculturally and economically more important, this manuscript provides essential reference intervals (RI) for their plasma biochemistry and hematology along with reference photomicrographs of blood cells in healthy, fasted sablefish. Blood cell morphology can differ between fish species. In addition, blood cell counts and blood chemistry can vary between fish species, demographics, water conditions, seasons, diets, and culture systems, which precludes the use of RI's from other fish species. For this study, blood was collected for plasma biochemistry and hematology analysis between June 20 and July 18, 2019, from healthy, yearling sablefish, hatched and reared in captivity on a commercial diet. Overnight fast of 16-18 hours did not sufficiently reduce lipids in the blood, which led to visible lipemia and frequent rupture of blood cells during analysis. Therefore, sablefish should be fasted for 24 to 36 hours before blood is collected to reduce hematology artifacts or possible reagent interference in plasma biochemistry analysis. Lymphocytes were the most dominant leukocytes (98%), while eosinophils were rare, and basophils were not detected in sablefish. Neutrophils were very large cells with Döhle bodies. In mammals and avian species, Döhle bodies are usually signs of toxic change from inflammation, but no such association was found in these fish. In conclusion, lipemia can interfere with sablefish blood analysis, and available removal methods should be evaluated as fasting for up to 36 h might not always be feasible. Also, more studies are required to establish RI for different developmental stages and rearing conditions. |
url |
https://doi.org/10.1371/journal.pone.0246982 |
work_keys_str_mv |
AT carlabschubiger sablefishanoplopomafimbrapallas1814plasmabiochemistryandhematologyreferenceintervalsincludingbloodcellmorphology AT melenagorman sablefishanoplopomafimbrapallas1814plasmabiochemistryandhematologyreferenceintervalsincludingbloodcellmorphology AT jenniferljohns sablefishanoplopomafimbrapallas1814plasmabiochemistryandhematologyreferenceintervalsincludingbloodcellmorphology AT maryrarkoosh sablefishanoplopomafimbrapallas1814plasmabiochemistryandhematologyreferenceintervalsincludingbloodcellmorphology AT josephpdietrich sablefishanoplopomafimbrapallas1814plasmabiochemistryandhematologyreferenceintervalsincludingbloodcellmorphology |
_version_ |
1721386267912962048 |