Distribution of Virulence Genes in <em>Salmonella</em> Serovars isolated from poultry farms in Kwara State, Nigeria
This study was aimed at investigating the prevalence and detection of virulence genes in <em>Salmonella </em>isolated from poultry farms in Kwara State, Nigeria. Ten commercial layer farms located in three senatorial districts of the state were sampled. The presence of <em>Salmonel...
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Faculty of Science, University of Peradeniya, Sri Lanka
2017-12-01
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doaj-67ebab0355e145be9d1e24e6800751302021-09-20T15:23:29ZengFaculty of Science, University of Peradeniya, Sri LankaCeylon Journal of Science2513-28142513-230X2017-12-01464697610.4038/cjs.v46i4.74695676Distribution of Virulence Genes in <em>Salmonella</em> Serovars isolated from poultry farms in Kwara State, NigeriaAhmed O. Akeem0P. H. Mamman1M. A. Raji2C. N. Kwanashie3I. A. Raufu4A. Aremu5University of Ilorin, Ilorin, Kwara StateAhmadu Bello University, Zaria, Kaduna StateUniversity of Ilorin, Ilorin, Kwara StateAhmadu Bello University, Zaria, Kaduna StateUniversity of Ilorin, Ilorin, Kwara StateUniversity of Ilorin, Ilorin, Kwara StateThis study was aimed at investigating the prevalence and detection of virulence genes in <em>Salmonella </em>isolated from poultry farms in Kwara State, Nigeria. Ten commercial layer farms located in three senatorial districts of the state were sampled. The presence of <em>Salmonella </em>was investigated using standard bacteriological techniques, the isolates obtained were serotyped and polymerase chain reaction (PCR) was used to detect presence of two genes (<em>fim</em>A and <em>sef</em>C) in the isolates. Of the 300 samples collected, 24 (8.0%) were positive for <em>Salmonella</em>; 4 different serovars were identified; <em>Salmonella </em>Larochelle, <em>S. </em>Muenster, <em>S. enterica </em>ser. 6, 7:d, <em>S. enterica </em>ser. 45:d:1,7 and <em>Salmonella </em>Typhimurium, the most frequent being <em>Salmonella </em>Typhimurium (3%). Varying distribution patterns of the studied genes were observed among the isolates. While, <em>fim</em>A was found in all the 24 (100%) isolates, <em>sef</em>C was found only among the <em>Salmonella enterica </em>ser. 45: d: 1,7. The study indicates that the <em>fim</em>A gene is widely distributed among <em>Salmonella </em>irrespective of the serovars. However, the <em>sef</em>C gene appears to be serovar specific. The prevalence of <em>Salmonella </em>in chicken and poultry farms constitutes a major public health concern. Hence, further epidemiological studies are necessary.https://cjs.sljol.info/articles/7469salmonella, kwara, virulence, poultry farms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahmed O. Akeem P. H. Mamman M. A. Raji C. N. Kwanashie I. A. Raufu A. Aremu |
spellingShingle |
Ahmed O. Akeem P. H. Mamman M. A. Raji C. N. Kwanashie I. A. Raufu A. Aremu Distribution of Virulence Genes in <em>Salmonella</em> Serovars isolated from poultry farms in Kwara State, Nigeria Ceylon Journal of Science salmonella, kwara, virulence, poultry farms |
author_facet |
Ahmed O. Akeem P. H. Mamman M. A. Raji C. N. Kwanashie I. A. Raufu A. Aremu |
author_sort |
Ahmed O. Akeem |
title |
Distribution of Virulence Genes in <em>Salmonella</em> Serovars isolated from poultry farms in Kwara State, Nigeria |
title_short |
Distribution of Virulence Genes in <em>Salmonella</em> Serovars isolated from poultry farms in Kwara State, Nigeria |
title_full |
Distribution of Virulence Genes in <em>Salmonella</em> Serovars isolated from poultry farms in Kwara State, Nigeria |
title_fullStr |
Distribution of Virulence Genes in <em>Salmonella</em> Serovars isolated from poultry farms in Kwara State, Nigeria |
title_full_unstemmed |
Distribution of Virulence Genes in <em>Salmonella</em> Serovars isolated from poultry farms in Kwara State, Nigeria |
title_sort |
distribution of virulence genes in <em>salmonella</em> serovars isolated from poultry farms in kwara state, nigeria |
publisher |
Faculty of Science, University of Peradeniya, Sri Lanka |
series |
Ceylon Journal of Science |
issn |
2513-2814 2513-230X |
publishDate |
2017-12-01 |
description |
This study was aimed at investigating the prevalence and detection of virulence genes in <em>Salmonella </em>isolated from poultry farms in Kwara State, Nigeria. Ten commercial layer farms located in three senatorial districts of the state were sampled. The presence of <em>Salmonella </em>was investigated using standard bacteriological techniques, the isolates obtained were serotyped and polymerase chain reaction (PCR) was used to detect presence of two genes (<em>fim</em>A and <em>sef</em>C) in the isolates. Of the 300 samples collected, 24 (8.0%) were positive for <em>Salmonella</em>; 4 different serovars were identified; <em>Salmonella </em>Larochelle, <em>S. </em>Muenster, <em>S. enterica </em>ser. 6, 7:d, <em>S. enterica </em>ser. 45:d:1,7 and <em>Salmonella </em>Typhimurium, the most frequent being <em>Salmonella </em>Typhimurium (3%). Varying distribution patterns of the studied genes were observed among the isolates. While, <em>fim</em>A was found in all the 24 (100%) isolates, <em>sef</em>C was found only among the <em>Salmonella enterica </em>ser. 45: d: 1,7. The study indicates that the <em>fim</em>A gene is widely distributed among <em>Salmonella </em>irrespective of the serovars. However, the <em>sef</em>C gene appears to be serovar specific. The prevalence of <em>Salmonella </em>in chicken and poultry farms constitutes a major public health concern. Hence, further epidemiological studies are necessary. |
topic |
salmonella, kwara, virulence, poultry farms |
url |
https://cjs.sljol.info/articles/7469 |
work_keys_str_mv |
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