A quality control of proteomic experiments based on multiple isotopologous internal standards

A quality control of proteomic experiments based on multiple isotopologous internal standards

The harmonization of proteomics experiments facilitates the exchange and comparison of results. The definition of standards and metrics ensures reliable and consistent data quality. An internal quality control procedure was developed to assess the different steps of a proteomic analysis workflow and...

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Bibliographic Details
Main Authors: Adele Bourmaud, Sebastien Gallien, Bruno Domon
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:EuPA Open Proteomics
Subjects:
Quality control
Internal standard
PRM
SRM
Isotopically labeled peptides
Online Access:http://www.sciencedirect.com/science/article/pii/S2212968515300131
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spelling doaj-67f8cf59464841e0bf5c0662e6cee21a2020-11-24T23:44:21ZengElsevierEuPA Open Proteomics2212-96852015-09-018C162110.1016/j.euprot.2015.07.010A quality control of proteomic experiments based on multiple isotopologous internal standardsAdele Bourmaud0Sebastien Gallien1Bruno Domon2Luxembourg Clinical Proteomics Center (LCP), Luxembourg Institute of Health (LIH), Strassen, LuxembourgLuxembourg Clinical Proteomics Center (LCP), Luxembourg Institute of Health (LIH), Strassen, LuxembourgLuxembourg Clinical Proteomics Center (LCP), Luxembourg Institute of Health (LIH), Strassen, LuxembourgThe harmonization of proteomics experiments facilitates the exchange and comparison of results. The definition of standards and metrics ensures reliable and consistent data quality. An internal quality control procedure was developed to assess the different steps of a proteomic analysis workflow and perform a system suitability test. The method relies on a straightforward protocol using a simple mixture of exogenous proteins, and the sequential addition of two sets of isotopically labeled peptides added to reference samples. This internal quality control procedure was applied to plasma samples to demonstrate its easy implementation, which makes it generic for most proteomics applications.http://www.sciencedirect.com/science/article/pii/S2212968515300131Quality controlInternal standardPRMSRMIsotopically labeled peptides
collection DOAJ
language English
format Article
sources DOAJ
author Adele Bourmaud
Sebastien Gallien
Bruno Domon
spellingShingle Adele Bourmaud
Sebastien Gallien
Bruno Domon
A quality control of proteomic experiments based on multiple isotopologous internal standards
EuPA Open Proteomics
Quality control
Internal standard
PRM
SRM
Isotopically labeled peptides
author_facet Adele Bourmaud
Sebastien Gallien
Bruno Domon
author_sort Adele Bourmaud
title A quality control of proteomic experiments based on multiple isotopologous internal standards
title_short A quality control of proteomic experiments based on multiple isotopologous internal standards
title_full A quality control of proteomic experiments based on multiple isotopologous internal standards
title_fullStr A quality control of proteomic experiments based on multiple isotopologous internal standards
title_full_unstemmed A quality control of proteomic experiments based on multiple isotopologous internal standards
title_sort quality control of proteomic experiments based on multiple isotopologous internal standards
publisher Elsevier
series EuPA Open Proteomics
issn 2212-9685
publishDate 2015-09-01
description The harmonization of proteomics experiments facilitates the exchange and comparison of results. The definition of standards and metrics ensures reliable and consistent data quality. An internal quality control procedure was developed to assess the different steps of a proteomic analysis workflow and perform a system suitability test. The method relies on a straightforward protocol using a simple mixture of exogenous proteins, and the sequential addition of two sets of isotopically labeled peptides added to reference samples. This internal quality control procedure was applied to plasma samples to demonstrate its easy implementation, which makes it generic for most proteomics applications.
topic Quality control
Internal standard
PRM
SRM
Isotopically labeled peptides
url http://www.sciencedirect.com/science/article/pii/S2212968515300131
work_keys_str_mv AT adelebourmaud aqualitycontrolofproteomicexperimentsbasedonmultipleisotopologousinternalstandards
AT sebastiengallien aqualitycontrolofproteomicexperimentsbasedonmultipleisotopologousinternalstandards
AT brunodomon aqualitycontrolofproteomicexperimentsbasedonmultipleisotopologousinternalstandards
AT adelebourmaud qualitycontrolofproteomicexperimentsbasedonmultipleisotopologousinternalstandards
AT sebastiengallien qualitycontrolofproteomicexperimentsbasedonmultipleisotopologousinternalstandards
AT brunodomon qualitycontrolofproteomicexperimentsbasedonmultipleisotopologousinternalstandards
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