Osteopontin reduces biofilm formation in a multi-species model of dental biofilm.

<h4>Background</h4>Combating dental biofilm formation is the most effective means for the prevention of caries, one of the most widespread human diseases. Among the chemical supplements to mechanical tooth cleaning procedures, non-bactericidal adjuncts that target the mechanisms of bacte...

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Main Authors: Sebastian Schlafer, Merete K Raarup, Peter L Wejse, Bente Nyvad, Brigitte M Städler, Duncan S Sutherland, Henrik Birkedal, Rikke L Meyer
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22879891/?tool=EBI
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spelling doaj-67f8ee1ee2704b73bc3994709981d31b2021-03-04T00:27:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4153410.1371/journal.pone.0041534Osteopontin reduces biofilm formation in a multi-species model of dental biofilm.Sebastian SchlaferMerete K RaarupPeter L WejseBente NyvadBrigitte M StädlerDuncan S SutherlandHenrik BirkedalRikke L Meyer<h4>Background</h4>Combating dental biofilm formation is the most effective means for the prevention of caries, one of the most widespread human diseases. Among the chemical supplements to mechanical tooth cleaning procedures, non-bactericidal adjuncts that target the mechanisms of bacterial biofilm formation have gained increasing interest in recent years. Milk proteins, such as lactoferrin, have been shown to interfere with bacterial colonization of saliva-coated surfaces. We here study the effect of bovine milk osteopontin (OPN), a highly phosphorylated whey glycoprotein, on a multispecies in vitro model of dental biofilm. While considerable research effort focuses on the interaction of OPN with mammalian cells, there are no data investigating the influence of OPN on bacterial biofilms.<h4>Methodology/principal findings</h4>Biofilms consisting of Streptococcus oralis, Actinomyces naeslundii, Streptococcus mitis, Streptococcus downei and Streptococcus sanguinis were grown in a flow cell system that permitted in situ microscopic analysis. Crystal violet staining showed significantly less biofilm formation in the presence of OPN, as compared to biofilms grown without OPN or biofilms grown in the presence of caseinoglycomacropeptide, another phosphorylated milk protein. Confocal microscopy revealed that OPN bound to the surface of bacterial cells and reduced mechanical stability of the biofilms without affecting cell viability. The bacterial composition of the biofilms, determined by fluorescence in situ hybridization, changed considerably in the presence of OPN. In particular, colonization of S. mitis, the best biofilm former in the model, was reduced dramatically.<h4>Conclusions/significance</h4>OPN strongly reduces the amount of biofilm formed in a well-defined laboratory model of acidogenic dental biofilm. If a similar effect can be observed in vivo, OPN might serve as a valuable adjunct to mechanical tooth cleaning procedures.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22879891/?tool=EBI
collection DOAJ
language English
format Article
sources DOAJ
author Sebastian Schlafer
Merete K Raarup
Peter L Wejse
Bente Nyvad
Brigitte M Städler
Duncan S Sutherland
Henrik Birkedal
Rikke L Meyer
spellingShingle Sebastian Schlafer
Merete K Raarup
Peter L Wejse
Bente Nyvad
Brigitte M Städler
Duncan S Sutherland
Henrik Birkedal
Rikke L Meyer
Osteopontin reduces biofilm formation in a multi-species model of dental biofilm.
PLoS ONE
author_facet Sebastian Schlafer
Merete K Raarup
Peter L Wejse
Bente Nyvad
Brigitte M Städler
Duncan S Sutherland
Henrik Birkedal
Rikke L Meyer
author_sort Sebastian Schlafer
title Osteopontin reduces biofilm formation in a multi-species model of dental biofilm.
title_short Osteopontin reduces biofilm formation in a multi-species model of dental biofilm.
title_full Osteopontin reduces biofilm formation in a multi-species model of dental biofilm.
title_fullStr Osteopontin reduces biofilm formation in a multi-species model of dental biofilm.
title_full_unstemmed Osteopontin reduces biofilm formation in a multi-species model of dental biofilm.
title_sort osteopontin reduces biofilm formation in a multi-species model of dental biofilm.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description <h4>Background</h4>Combating dental biofilm formation is the most effective means for the prevention of caries, one of the most widespread human diseases. Among the chemical supplements to mechanical tooth cleaning procedures, non-bactericidal adjuncts that target the mechanisms of bacterial biofilm formation have gained increasing interest in recent years. Milk proteins, such as lactoferrin, have been shown to interfere with bacterial colonization of saliva-coated surfaces. We here study the effect of bovine milk osteopontin (OPN), a highly phosphorylated whey glycoprotein, on a multispecies in vitro model of dental biofilm. While considerable research effort focuses on the interaction of OPN with mammalian cells, there are no data investigating the influence of OPN on bacterial biofilms.<h4>Methodology/principal findings</h4>Biofilms consisting of Streptococcus oralis, Actinomyces naeslundii, Streptococcus mitis, Streptococcus downei and Streptococcus sanguinis were grown in a flow cell system that permitted in situ microscopic analysis. Crystal violet staining showed significantly less biofilm formation in the presence of OPN, as compared to biofilms grown without OPN or biofilms grown in the presence of caseinoglycomacropeptide, another phosphorylated milk protein. Confocal microscopy revealed that OPN bound to the surface of bacterial cells and reduced mechanical stability of the biofilms without affecting cell viability. The bacterial composition of the biofilms, determined by fluorescence in situ hybridization, changed considerably in the presence of OPN. In particular, colonization of S. mitis, the best biofilm former in the model, was reduced dramatically.<h4>Conclusions/significance</h4>OPN strongly reduces the amount of biofilm formed in a well-defined laboratory model of acidogenic dental biofilm. If a similar effect can be observed in vivo, OPN might serve as a valuable adjunct to mechanical tooth cleaning procedures.
url https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22879891/?tool=EBI
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