Investigating the Lymphatic System by Dual-Color Elemental Mass Spectrometry Imaging
Secondary lymphedema accompanied with strong restrictions in quality of life is still major side effects in cancer therapy. Therefore, dedicated diagnostic tools and further investigation of the lymphatic system are crucial to improve lymphedema therapy. In this pilot study, a method for quantitativ...
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Series: | Contrast Media & Molecular Imaging |
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doaj-6802a17856fa43709c2d21f2b09f0ef92020-11-25T01:02:06ZengHindawi-WileyContrast Media & Molecular Imaging1555-43091555-43172017-01-01201710.1155/2017/40357214035721Investigating the Lymphatic System by Dual-Color Elemental Mass Spectrometry ImagingAnn-Christin Niehoff0Tim Klasen1Rebecca Schmidt2Daniel Palmes3Cornelius Faber4Uwe Karst5Rebecca Hadrian6Institute of Inorganic and Analytical Chemistry, University of Muenster, 48149 Muenster, GermanyDepartment of Clinical Radiology, University Hospital Muenster, 48149 Muenster, GermanyDepartment of Clinical Radiology, University Hospital Muenster, 48149 Muenster, GermanyDepartment of General and Visceral Surgery, University Hospital Muenster, 48149 Muenster, GermanyDepartment of Clinical Radiology, University Hospital Muenster, 48149 Muenster, GermanyInstitute of Inorganic and Analytical Chemistry, University of Muenster, 48149 Muenster, GermanyDepartment of General and Visceral Surgery, University Hospital Muenster, 48149 Muenster, GermanySecondary lymphedema accompanied with strong restrictions in quality of life is still major side effects in cancer therapy. Therefore, dedicated diagnostic tools and further investigation of the lymphatic system are crucial to improve lymphedema therapy. In this pilot study, a method for quantitative analysis of the lymphatic system in a rat model by laser ablation (LA) with inductively coupled plasma mass spectrometry imaging (ICP-MSI) is presented. As a possible lymph marker, thulium(III)(1R,4R,7R,10R)-α,α′,α′′,α′′′-tetramethyl-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (Tm-DOTMA) is introduced and compared to the clinically used magnetic resonance imaging contrast agent gadolinium(III)2,2′,2′′-(10-((2R,3S)-1,3,4-trihydroxybutan-2-yl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetate (Gd-DO3A-butrol). Gadobutrol functioned as standard contrast media in MRI lymphangiography to detect lymphatic flow qualitatively. Thus, Tm-DOTMA was investigated as lymphatic marker to detect lymphatic flow quantitatively. Both contrast agents were successfully used to visualize the lymphatic flow in successive lymph nodes in LA-ICP-MS due to lower limits of detection compared to MRI. Furthermore, the distribution of contrast agents by multicolored imaging showed accumulation in specific areas (sectors) of the lymph nodes after application of contrast agents in different areas.http://dx.doi.org/10.1155/2017/4035721 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ann-Christin Niehoff Tim Klasen Rebecca Schmidt Daniel Palmes Cornelius Faber Uwe Karst Rebecca Hadrian |
spellingShingle |
Ann-Christin Niehoff Tim Klasen Rebecca Schmidt Daniel Palmes Cornelius Faber Uwe Karst Rebecca Hadrian Investigating the Lymphatic System by Dual-Color Elemental Mass Spectrometry Imaging Contrast Media & Molecular Imaging |
author_facet |
Ann-Christin Niehoff Tim Klasen Rebecca Schmidt Daniel Palmes Cornelius Faber Uwe Karst Rebecca Hadrian |
author_sort |
Ann-Christin Niehoff |
title |
Investigating the Lymphatic System by Dual-Color Elemental Mass Spectrometry Imaging |
title_short |
Investigating the Lymphatic System by Dual-Color Elemental Mass Spectrometry Imaging |
title_full |
Investigating the Lymphatic System by Dual-Color Elemental Mass Spectrometry Imaging |
title_fullStr |
Investigating the Lymphatic System by Dual-Color Elemental Mass Spectrometry Imaging |
title_full_unstemmed |
Investigating the Lymphatic System by Dual-Color Elemental Mass Spectrometry Imaging |
title_sort |
investigating the lymphatic system by dual-color elemental mass spectrometry imaging |
publisher |
Hindawi-Wiley |
series |
Contrast Media & Molecular Imaging |
issn |
1555-4309 1555-4317 |
publishDate |
2017-01-01 |
description |
Secondary lymphedema accompanied with strong restrictions in quality of life is still major side effects in cancer therapy. Therefore, dedicated diagnostic tools and further investigation of the lymphatic system are crucial to improve lymphedema therapy. In this pilot study, a method for quantitative analysis of the lymphatic system in a rat model by laser ablation (LA) with inductively coupled plasma mass spectrometry imaging (ICP-MSI) is presented. As a possible lymph marker, thulium(III)(1R,4R,7R,10R)-α,α′,α′′,α′′′-tetramethyl-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (Tm-DOTMA) is introduced and compared to the clinically used magnetic resonance imaging contrast agent gadolinium(III)2,2′,2′′-(10-((2R,3S)-1,3,4-trihydroxybutan-2-yl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetate (Gd-DO3A-butrol). Gadobutrol functioned as standard contrast media in MRI lymphangiography to detect lymphatic flow qualitatively. Thus, Tm-DOTMA was investigated as lymphatic marker to detect lymphatic flow quantitatively. Both contrast agents were successfully used to visualize the lymphatic flow in successive lymph nodes in LA-ICP-MS due to lower limits of detection compared to MRI. Furthermore, the distribution of contrast agents by multicolored imaging showed accumulation in specific areas (sectors) of the lymph nodes after application of contrast agents in different areas. |
url |
http://dx.doi.org/10.1155/2017/4035721 |
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