Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro
It has been widely shown that biomaterial surface topography can modulate host immune response, but a fundamental understanding of how different topographies contribute to pro-inflammatory or anti-inflammatory responses is still lacking. To investigate the impact of surface topography on immune resp...
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Hindawi Limited
2020-01-01
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Series: | BioMed Research International |
Online Access: | http://dx.doi.org/10.1155/2020/3481549 |
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doaj-6803936903d04dcd9bb4917fecac1d62 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sören Segan Meike Jakobi Paree Khokhani Sascha Klimosch Florian Billing Markus Schneider Dagmar Martin Ute Metzger Antje Biesemeier Xin Xiong Ashutosh Mukherjee Heiko Steuer Bettina-Maria Keller Thomas Joos Manfred Schmolz Ulrich Rothbauer Hanna Hartmann Claus Burkhardt Günter Lorenz Nicole Schneiderhan-Marra Christopher Shipp |
spellingShingle |
Sören Segan Meike Jakobi Paree Khokhani Sascha Klimosch Florian Billing Markus Schneider Dagmar Martin Ute Metzger Antje Biesemeier Xin Xiong Ashutosh Mukherjee Heiko Steuer Bettina-Maria Keller Thomas Joos Manfred Schmolz Ulrich Rothbauer Hanna Hartmann Claus Burkhardt Günter Lorenz Nicole Schneiderhan-Marra Christopher Shipp Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro BioMed Research International |
author_facet |
Sören Segan Meike Jakobi Paree Khokhani Sascha Klimosch Florian Billing Markus Schneider Dagmar Martin Ute Metzger Antje Biesemeier Xin Xiong Ashutosh Mukherjee Heiko Steuer Bettina-Maria Keller Thomas Joos Manfred Schmolz Ulrich Rothbauer Hanna Hartmann Claus Burkhardt Günter Lorenz Nicole Schneiderhan-Marra Christopher Shipp |
author_sort |
Sören Segan |
title |
Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro |
title_short |
Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro |
title_full |
Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro |
title_fullStr |
Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro |
title_full_unstemmed |
Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro |
title_sort |
systematic investigation of polyurethane biomaterial surface roughness on human immune responses in vitro |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2020-01-01 |
description |
It has been widely shown that biomaterial surface topography can modulate host immune response, but a fundamental understanding of how different topographies contribute to pro-inflammatory or anti-inflammatory responses is still lacking. To investigate the impact of surface topography on immune response, we undertook a systematic approach by analyzing immune response to eight grades of medical grade polyurethane of increasing surface roughness in three in vitro models of the human immune system. Polyurethane specimens were produced with defined roughness values by injection molding according to the VDI 3400 industrial standard. Specimens ranged from 0.1 μm to 18 μm in average roughness (Ra), which was confirmed by confocal scanning microscopy. Immunological responses were assessed with THP-1-derived macrophages, human peripheral blood mononuclear cells (PBMCs), and whole blood following culture on polyurethane specimens. As shown by the release of pro-inflammatory and anti-inflammatory cytokines in all three models, a mild immune response to polyurethane was observed, however, this was not associated with the degree of surface roughness. Likewise, the cell morphology (cell spreading, circularity, and elongation) in THP-1-derived macrophages and the expression of CD molecules in the PBMC model on T cells (HLA-DR and CD16), NK cells (HLA-DR), and monocytes (HLA-DR, CD16, CD86, and CD163) showed no influence of surface roughness. In summary, this study shows that modifying surface roughness in the micrometer range on polyurethane has no impact on the pro-inflammatory immune response. Therefore, we propose that such modifications do not affect the immunocompatibility of polyurethane, thereby supporting the notion of polyurethane as a biocompatible material. |
url |
http://dx.doi.org/10.1155/2020/3481549 |
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doaj-6803936903d04dcd9bb4917fecac1d622020-11-25T02:32:49ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/34815493481549Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitroSören Segan0Meike Jakobi1Paree Khokhani2Sascha Klimosch3Florian Billing4Markus Schneider5Dagmar Martin6Ute Metzger7Antje Biesemeier8Xin Xiong9Ashutosh Mukherjee10Heiko Steuer11Bettina-Maria Keller12Thomas Joos13Manfred Schmolz14Ulrich Rothbauer15Hanna Hartmann16Claus Burkhardt17Günter Lorenz18Nicole Schneiderhan-Marra19Christopher Shipp20NMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyUniversity of Applied Sciences, Reutlingen, Alteburgstr. 150, 72762 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyUniversity of Tübingen, Geschwister-Scholl-Platz, 72074 Tübingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyHOT Screen GmbH, Aspenhaustraße 25, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyUniversity of Applied Sciences, Reutlingen, Alteburgstr. 150, 72762 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyNMI, Natural and Medical Sciences Institute at the University of Tübingen, Markwiesenstr. 55, 72770 Reutlingen, GermanyIt has been widely shown that biomaterial surface topography can modulate host immune response, but a fundamental understanding of how different topographies contribute to pro-inflammatory or anti-inflammatory responses is still lacking. To investigate the impact of surface topography on immune response, we undertook a systematic approach by analyzing immune response to eight grades of medical grade polyurethane of increasing surface roughness in three in vitro models of the human immune system. Polyurethane specimens were produced with defined roughness values by injection molding according to the VDI 3400 industrial standard. Specimens ranged from 0.1 μm to 18 μm in average roughness (Ra), which was confirmed by confocal scanning microscopy. Immunological responses were assessed with THP-1-derived macrophages, human peripheral blood mononuclear cells (PBMCs), and whole blood following culture on polyurethane specimens. As shown by the release of pro-inflammatory and anti-inflammatory cytokines in all three models, a mild immune response to polyurethane was observed, however, this was not associated with the degree of surface roughness. Likewise, the cell morphology (cell spreading, circularity, and elongation) in THP-1-derived macrophages and the expression of CD molecules in the PBMC model on T cells (HLA-DR and CD16), NK cells (HLA-DR), and monocytes (HLA-DR, CD16, CD86, and CD163) showed no influence of surface roughness. In summary, this study shows that modifying surface roughness in the micrometer range on polyurethane has no impact on the pro-inflammatory immune response. Therefore, we propose that such modifications do not affect the immunocompatibility of polyurethane, thereby supporting the notion of polyurethane as a biocompatible material.http://dx.doi.org/10.1155/2020/3481549 |