Safety and tolerability of MRI-guided infusion of AAV2-hAADC into the mid-brain of nonhuman primate
Aromatic L-amino acid decarboxylase (AADC) deficiency is a rare, autosomal-recessive neurological disorder caused by mutations in the DDC gene that leads to an inability to synthesize catecholamines and serotonin. As a result, patients suffer compromised development, particularly in motor function....
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doaj-6808879212064f8090e19ab0d9b2d62a2020-11-25T00:07:55ZengElsevierMolecular Therapy: Methods & Clinical Development2329-05012014-01-011C10.1038/mtm.2014.49Safety and tolerability of MRI-guided infusion of AAV2-hAADC into the mid-brain of nonhuman primateWaldy San Sebastian0Adrian P Kells1John Bringas2Lluis Samaranch3Piotr Hadaczek4Agnieszka Ciesielska5Michael J Macayan6Phillip J Pivirotto7John Forsayeth8Sheryl Osborne9J Fraser Wright10Foad Green11Gregory Heller12Krystof S Bankiewicz13Department of Neurological Surgery, University of California San Francisco, San Francisco, California, USADepartment of Neurological Surgery, University of California San Francisco, San Francisco, California, USADepartment of Neurological Surgery, University of California San Francisco, San Francisco, California, USADepartment of Neurological Surgery, University of California San Francisco, San Francisco, California, USADepartment of Neurological Surgery, University of California San Francisco, San Francisco, California, USADepartment of Neurological Surgery, University of California San Francisco, San Francisco, California, USADepartment of Neurological Surgery, University of California San Francisco, San Francisco, California, USADepartment of Neurological Surgery, University of California San Francisco, San Francisco, California, USADepartment of Neurological Surgery, University of California San Francisco, San Francisco, California, USAConsultantCenter for Cellular and Molecular Therapeutics, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USADepartment of Neurological Surgery, University of California San Francisco, San Francisco, California, USADepartment of Neurological Surgery, University of California San Francisco, San Francisco, California, USADepartment of Neurological Surgery, University of California San Francisco, San Francisco, California, USAAromatic L-amino acid decarboxylase (AADC) deficiency is a rare, autosomal-recessive neurological disorder caused by mutations in the DDC gene that leads to an inability to synthesize catecholamines and serotonin. As a result, patients suffer compromised development, particularly in motor function. A recent gene replacement clinical trial explored putaminal delivery of recombinant adeno-associated virus serotype 2 vector encoding human AADC (AAV2-hAADC) in AADC-deficient children. Unfortunately, patients presented only modest amelioration of motor symptoms, which authors acknowledged could be due to insufficient transduction of putamen. We hypothesize that, with the development of a highly accurate MRI-guided cannula placement technology, a more effective approach might be to target the affected mid-brain neurons directly. Transduction of AADC-deficient dopaminergic neurons in the substantia nigra and ventral tegmental area with locally infused AAV2-hAADC would be expected to lead to restoration of normal dopamine levels in affected children. The objective of this study was to assess the long-term safety and tolerability of bilateral AAV2-hAADC MRI-guided pressurized infusion into the mid-brain of nonhuman primates. Animals received either vehicle, low or high AAV2-hAADC vector dose and were euthanized 1, 3, or 9 months after surgery. Our data indicate that effective mid-brain transduction was achieved without untoward effects.http://www.sciencedirect.com/science/article/pii/S2329050116301176 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Waldy San Sebastian Adrian P Kells John Bringas Lluis Samaranch Piotr Hadaczek Agnieszka Ciesielska Michael J Macayan Phillip J Pivirotto John Forsayeth Sheryl Osborne J Fraser Wright Foad Green Gregory Heller Krystof S Bankiewicz |
spellingShingle |
Waldy San Sebastian Adrian P Kells John Bringas Lluis Samaranch Piotr Hadaczek Agnieszka Ciesielska Michael J Macayan Phillip J Pivirotto John Forsayeth Sheryl Osborne J Fraser Wright Foad Green Gregory Heller Krystof S Bankiewicz Safety and tolerability of MRI-guided infusion of AAV2-hAADC into the mid-brain of nonhuman primate Molecular Therapy: Methods & Clinical Development |
author_facet |
Waldy San Sebastian Adrian P Kells John Bringas Lluis Samaranch Piotr Hadaczek Agnieszka Ciesielska Michael J Macayan Phillip J Pivirotto John Forsayeth Sheryl Osborne J Fraser Wright Foad Green Gregory Heller Krystof S Bankiewicz |
author_sort |
Waldy San Sebastian |
title |
Safety and tolerability of MRI-guided infusion of AAV2-hAADC into the mid-brain of nonhuman primate |
title_short |
Safety and tolerability of MRI-guided infusion of AAV2-hAADC into the mid-brain of nonhuman primate |
title_full |
Safety and tolerability of MRI-guided infusion of AAV2-hAADC into the mid-brain of nonhuman primate |
title_fullStr |
Safety and tolerability of MRI-guided infusion of AAV2-hAADC into the mid-brain of nonhuman primate |
title_full_unstemmed |
Safety and tolerability of MRI-guided infusion of AAV2-hAADC into the mid-brain of nonhuman primate |
title_sort |
safety and tolerability of mri-guided infusion of aav2-haadc into the mid-brain of nonhuman primate |
publisher |
Elsevier |
series |
Molecular Therapy: Methods & Clinical Development |
issn |
2329-0501 |
publishDate |
2014-01-01 |
description |
Aromatic L-amino acid decarboxylase (AADC) deficiency is a rare, autosomal-recessive neurological disorder caused by mutations in the DDC gene that leads to an inability to synthesize catecholamines and serotonin. As a result, patients suffer compromised development, particularly in motor function. A recent gene replacement clinical trial explored putaminal delivery of recombinant adeno-associated virus serotype 2 vector encoding human AADC (AAV2-hAADC) in AADC-deficient children. Unfortunately, patients presented only modest amelioration of motor symptoms, which authors acknowledged could be due to insufficient transduction of putamen. We hypothesize that, with the development of a highly accurate MRI-guided cannula placement technology, a more effective approach might be to target the affected mid-brain neurons directly. Transduction of AADC-deficient dopaminergic neurons in the substantia nigra and ventral tegmental area with locally infused AAV2-hAADC would be expected to lead to restoration of normal dopamine levels in affected children. The objective of this study was to assess the long-term safety and tolerability of bilateral AAV2-hAADC MRI-guided pressurized infusion into the mid-brain of nonhuman primates. Animals received either vehicle, low or high AAV2-hAADC vector dose and were euthanized 1, 3, or 9 months after surgery. Our data indicate that effective mid-brain transduction was achieved without untoward effects. |
url |
http://www.sciencedirect.com/science/article/pii/S2329050116301176 |
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