Clinical Characteristics and Genotypes of Rotaviruses in a Neonatal Intensive Care Unit
There are few reports on the symptoms of rotavirus infections in neonates. This study aims to describe clinical signs of rotavirus infections among neonates, with a particular focus on preterm infants, and to show the distribution of genotypes in a neonatal intensive care unit (NICU). Methods: A pro...
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doaj-6828e57c520241ada2c0c32993ba06aa2020-11-24T21:23:44ZengElsevierPediatrics and Neonatology1875-95722012-02-01531182310.1016/j.pedneo.2011.11.005Clinical Characteristics and Genotypes of Rotaviruses in a Neonatal Intensive Care UnitJung Ok Shim0Dong Woo Son1So-Yeon Shim2Eell Ryoo3Wonyong Kim4Yeon-Chang Jung5Department of Pediatrics, Kangwon National University School of Medicine, 200-949 Chuncheon, South KoreaDepartment of Pediatrics, Gachon University of Medicine and Science, 405-760 Incheon, South KoreaDepartment of Pediatrics, Gachon University of Medicine and Science, 405-760 Incheon, South KoreaDepartment of Pediatrics, Gachon University of Medicine and Science, 405-760 Incheon, South KoreaDepartment of Microbiology, Chung-Ang University College of Medicine, 156-756 Seoul, South KoreaDepartment of Microbiology, Chung-Ang University College of Medicine, 156-756 Seoul, South KoreaThere are few reports on the symptoms of rotavirus infections in neonates. This study aims to describe clinical signs of rotavirus infections among neonates, with a particular focus on preterm infants, and to show the distribution of genotypes in a neonatal intensive care unit (NICU). Methods: A prospective observational study was conducted at a regional NICU for 1 year. Stool specimens from every infant in the NICU were collected on admission, at weekly intervals, and from infants showing symptoms. Rotavirus antigens were detected by enzyme-linked immunosorbent assay (ELISA), and genotypes were confirmed by Reverse transcription-Polymerase chain reaction (RT-PCR). The infants were divided into three groups: symptomatic preterm infants with and without rotavirus-positive stools [Preterm(rota+) and Preterm(rota–), respectively] and symptomatic full- or near-term infants with rotavirus-positive stools [FT/NT(rota+)]. Demographic and outcome data were compared among these groups. Results: A total of 702 infants were evaluated for rotaviruses and 131 infants were included in this study. The prevalence of rotavirus infections was 25.2%. Preterm(rota+) differed from Preterm(rota–) and FT/NT(rota+) with respect to frequent feeding difficulty (p = 0.047 and 0.034, respectively) and higher percentage of neutropenia (p = 0.008 and 0.011, respectively). G4P[6] was the exclusive strain in both the Preterm(rota+) (97.7%) and FT/NT(rota+) (90.2%), and it was the same for nosocomial, institutional infections, and infections acquired at home. Conclusion: Systemic illness signs such as feeding difficulty and neutropenia are specific for preterm infants with rotavirus infections. G4P[6] was exclusive, regardless of preterm birth or locations of infections. This study might be helpful in developing policies for management and prevention of rotavirus infections in NICUs.http://www.sciencedirect.com/science/article/pii/S1875957211001690clinical symptomgenotypenosocomialpreterm infantsrotavirus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jung Ok Shim Dong Woo Son So-Yeon Shim Eell Ryoo Wonyong Kim Yeon-Chang Jung |
spellingShingle |
Jung Ok Shim Dong Woo Son So-Yeon Shim Eell Ryoo Wonyong Kim Yeon-Chang Jung Clinical Characteristics and Genotypes of Rotaviruses in a Neonatal Intensive Care Unit Pediatrics and Neonatology clinical symptom genotype nosocomial preterm infants rotavirus |
author_facet |
Jung Ok Shim Dong Woo Son So-Yeon Shim Eell Ryoo Wonyong Kim Yeon-Chang Jung |
author_sort |
Jung Ok Shim |
title |
Clinical Characteristics and Genotypes of Rotaviruses in a Neonatal Intensive Care Unit |
title_short |
Clinical Characteristics and Genotypes of Rotaviruses in a Neonatal Intensive Care Unit |
title_full |
Clinical Characteristics and Genotypes of Rotaviruses in a Neonatal Intensive Care Unit |
title_fullStr |
Clinical Characteristics and Genotypes of Rotaviruses in a Neonatal Intensive Care Unit |
title_full_unstemmed |
Clinical Characteristics and Genotypes of Rotaviruses in a Neonatal Intensive Care Unit |
title_sort |
clinical characteristics and genotypes of rotaviruses in a neonatal intensive care unit |
publisher |
Elsevier |
series |
Pediatrics and Neonatology |
issn |
1875-9572 |
publishDate |
2012-02-01 |
description |
There are few reports on the symptoms of rotavirus infections in neonates. This study aims to describe clinical signs of rotavirus infections among neonates, with a particular focus on preterm infants, and to show the distribution of genotypes in a neonatal intensive care unit (NICU).
Methods: A prospective observational study was conducted at a regional NICU for 1 year. Stool specimens from every infant in the NICU were collected on admission, at weekly intervals, and from infants showing symptoms. Rotavirus antigens were detected by enzyme-linked immunosorbent assay (ELISA), and genotypes were confirmed by Reverse transcription-Polymerase chain reaction (RT-PCR). The infants were divided into three groups: symptomatic preterm infants with and without rotavirus-positive stools [Preterm(rota+) and Preterm(rota–), respectively] and symptomatic full- or near-term infants with rotavirus-positive stools [FT/NT(rota+)]. Demographic and outcome data were compared among these groups.
Results: A total of 702 infants were evaluated for rotaviruses and 131 infants were included in this study. The prevalence of rotavirus infections was 25.2%. Preterm(rota+) differed from Preterm(rota–) and FT/NT(rota+) with respect to frequent feeding difficulty (p = 0.047 and 0.034, respectively) and higher percentage of neutropenia (p = 0.008 and 0.011, respectively). G4P[6] was the exclusive strain in both the Preterm(rota+) (97.7%) and FT/NT(rota+) (90.2%), and it was the same for nosocomial, institutional infections, and infections acquired at home.
Conclusion: Systemic illness signs such as feeding difficulty and neutropenia are specific for preterm infants with rotavirus infections. G4P[6] was exclusive, regardless of preterm birth or locations of infections. This study might be helpful in developing policies for management and prevention of rotavirus infections in NICUs. |
topic |
clinical symptom genotype nosocomial preterm infants rotavirus |
url |
http://www.sciencedirect.com/science/article/pii/S1875957211001690 |
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