Characterization of Translationally Controlled Tumour Protein from the Sea Anemone Anemonia viridis and Transcriptome Wide Identification of Cnidarian Homologues
Gene family encoding translationally controlled tumour protein (TCTP) is defined as highly conserved among organisms; however, there is limited knowledge of non-bilateria. In this study, the first TCTP homologue from anthozoan was characterised in the Mediterranean Sea anemone, Anemonia viridis. The...
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doaj-682b1309ca5640eca37d2bdcf68039f02020-11-24T23:18:47ZengMDPI AGGenes2073-44252018-01-01913010.3390/genes9010030genes9010030Characterization of Translationally Controlled Tumour Protein from the Sea Anemone Anemonia viridis and Transcriptome Wide Identification of Cnidarian HomologuesAldo Nicosia0Carmelo Bennici1Girolama Biondo2Salvatore Costa3Marilena Di Natale4Tiziana Masullo5Calogera Monastero6Maria Antonietta Ragusa7Marcello Tagliavia8Angela Cuttitta9National Research Council-Institute for Marine and Coastal Environment (IAMC-CNR), Laboratory of Molecular Ecology and Biotechnology, Detached Unit of Capo Granitola, Via del mare, 91021 Torretta Granitola (TP), Sicily, ItalyNational Research Council-Institute for Marine and Coastal Environment (IAMC-CNR), Laboratory of Molecular Ecology and Biotechnology, Detached Unit of Capo Granitola, Via del mare, 91021 Torretta Granitola (TP), Sicily, ItalyNational Research Council-Institute for Marine and Coastal Environment (IAMC-CNR), Laboratory of Molecular Ecology and Biotechnology, Detached Unit of Capo Granitola, Via del mare, 91021 Torretta Granitola (TP), Sicily, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Sicily, ItalyNational Research Council-Institute for Marine and Coastal Environment (IAMC-CNR), Laboratory of Molecular Ecology and Biotechnology, Detached Unit of Capo Granitola, Via del mare, 91021 Torretta Granitola (TP), Sicily, ItalyNational Research Council-Institute for Marine and Coastal Environment (IAMC-CNR), Laboratory of Molecular Ecology and Biotechnology, Detached Unit of Capo Granitola, Via del mare, 91021 Torretta Granitola (TP), Sicily, ItalyNational Research Council-Institute for Marine and Coastal Environment (IAMC-CNR), Laboratory of Molecular Ecology and Biotechnology, Detached Unit of Capo Granitola, Via del mare, 91021 Torretta Granitola (TP), Sicily, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies, University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Sicily, ItalyNational Research Council-Institute for Marine and Coastal Environment (IAMC-CNR), Laboratory of Molecular Ecology and Biotechnology, Detached Unit of Capo Granitola, Via del mare, 91021 Torretta Granitola (TP), Sicily, ItalyNational Research Council-Institute for Marine and Coastal Environment (IAMC-CNR), Laboratory of Molecular Ecology and Biotechnology, Detached Unit of Capo Granitola, Via del mare, 91021 Torretta Granitola (TP), Sicily, ItalyGene family encoding translationally controlled tumour protein (TCTP) is defined as highly conserved among organisms; however, there is limited knowledge of non-bilateria. In this study, the first TCTP homologue from anthozoan was characterised in the Mediterranean Sea anemone, Anemonia viridis. The release of the genome sequence of Acropora digitifera, Exaiptasia pallida, Nematostella vectensis and Hydra vulgaris enabled a comprehensive study of the molecular evolution of TCTP family among cnidarians. A comparison among TCTP members from Cnidaria and Bilateria showed conserved intron exon organization, evolutionary conserved TCTP signatures and 3D protein structure. The pattern of mRNA expression profile was also defined in A. viridis. These analyses revealed a constitutive mRNA expression especially in tissues with active proliferation. Additionally, the transcriptional profile of A. viridis TCTP (AvTCTP) after challenges with different abiotic/biotic stresses showed induction by extreme temperatures, heavy metals exposure and immune stimulation. These results suggest the involvement of AvTCTP in the sea anemone defensome taking part in environmental stress and immune responses.http://www.mdpi.com/2073-4425/9/1/30cnidarianstranscriptome wide analysistranslationally controlled tumour proteinTCTPscomparative genomicshomology modellinggene expression |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aldo Nicosia Carmelo Bennici Girolama Biondo Salvatore Costa Marilena Di Natale Tiziana Masullo Calogera Monastero Maria Antonietta Ragusa Marcello Tagliavia Angela Cuttitta |
spellingShingle |
Aldo Nicosia Carmelo Bennici Girolama Biondo Salvatore Costa Marilena Di Natale Tiziana Masullo Calogera Monastero Maria Antonietta Ragusa Marcello Tagliavia Angela Cuttitta Characterization of Translationally Controlled Tumour Protein from the Sea Anemone Anemonia viridis and Transcriptome Wide Identification of Cnidarian Homologues Genes cnidarians transcriptome wide analysis translationally controlled tumour protein TCTPs comparative genomics homology modelling gene expression |
author_facet |
Aldo Nicosia Carmelo Bennici Girolama Biondo Salvatore Costa Marilena Di Natale Tiziana Masullo Calogera Monastero Maria Antonietta Ragusa Marcello Tagliavia Angela Cuttitta |
author_sort |
Aldo Nicosia |
title |
Characterization of Translationally Controlled Tumour Protein from the Sea Anemone Anemonia viridis and Transcriptome Wide Identification of Cnidarian Homologues |
title_short |
Characterization of Translationally Controlled Tumour Protein from the Sea Anemone Anemonia viridis and Transcriptome Wide Identification of Cnidarian Homologues |
title_full |
Characterization of Translationally Controlled Tumour Protein from the Sea Anemone Anemonia viridis and Transcriptome Wide Identification of Cnidarian Homologues |
title_fullStr |
Characterization of Translationally Controlled Tumour Protein from the Sea Anemone Anemonia viridis and Transcriptome Wide Identification of Cnidarian Homologues |
title_full_unstemmed |
Characterization of Translationally Controlled Tumour Protein from the Sea Anemone Anemonia viridis and Transcriptome Wide Identification of Cnidarian Homologues |
title_sort |
characterization of translationally controlled tumour protein from the sea anemone anemonia viridis and transcriptome wide identification of cnidarian homologues |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2018-01-01 |
description |
Gene family encoding translationally controlled tumour protein (TCTP) is defined as highly conserved among organisms; however, there is limited knowledge of non-bilateria. In this study, the first TCTP homologue from anthozoan was characterised in the Mediterranean Sea anemone, Anemonia viridis. The release of the genome sequence of Acropora digitifera, Exaiptasia pallida, Nematostella vectensis and Hydra vulgaris enabled a comprehensive study of the molecular evolution of TCTP family among cnidarians. A comparison among TCTP members from Cnidaria and Bilateria showed conserved intron exon organization, evolutionary conserved TCTP signatures and 3D protein structure. The pattern of mRNA expression profile was also defined in A. viridis. These analyses revealed a constitutive mRNA expression especially in tissues with active proliferation. Additionally, the transcriptional profile of A. viridis TCTP (AvTCTP) after challenges with different abiotic/biotic stresses showed induction by extreme temperatures, heavy metals exposure and immune stimulation. These results suggest the involvement of AvTCTP in the sea anemone defensome taking part in environmental stress and immune responses. |
topic |
cnidarians transcriptome wide analysis translationally controlled tumour protein TCTPs comparative genomics homology modelling gene expression |
url |
http://www.mdpi.com/2073-4425/9/1/30 |
work_keys_str_mv |
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