Optimization of Enzymatic Hydrolysis of Bee Pollen Protein by Pepsin Based on Antioxidant and ACE Inhibitory Activity and Comparison with Those of Royal Jelly

In this study optimization of the enzymatic hydrolysis condition of bee pollen protein by pepsin and the effect of enzymatic hydrolysis on its antioxidant properties was investigated. Also, the results were compared with antioxidant properties of royal jelly. For this purpose, phenolic compounds, DP...

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Main Authors: Atefe Maqsoudlou, Alireza Sadeghi Mahoonak, Mohammad Ghorbani, Fidel Toldra
Format: Article
Language:fas
Published: Research Institute of Food Science and Technology 2018-05-01
Series:Pizhūhish va Nuāvarī dar ̒Ulūm va Sanāyi̒-i Ghaz̠āyī
Subjects:
ace
Online Access:http://journals.rifst.ac.ir/article_67046_2b7c14c388ac84a2047fe8e96f50766e.pdf
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spelling doaj-6831ba17a3ff417b9454e9e98a13c7332020-11-25T01:26:51ZfasResearch Institute of Food Science and Technology Pizhūhish va Nuāvarī dar ̒Ulūm va Sanāyi̒-i Ghaz̠āyī2252-09372538-23572018-05-0171496410.22101/jrifst.2018.05.19.71467046Optimization of Enzymatic Hydrolysis of Bee Pollen Protein by Pepsin Based on Antioxidant and ACE Inhibitory Activity and Comparison with Those of Royal JellyAtefe Maqsoudlou0Alireza Sadeghi Mahoonak1Mohammad Ghorbani2Fidel Toldra3PhD Student, Department of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, IranAssociate, Department of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, IranAssociate, Department of Food Science & Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, IranProfessor -Instituto de Agroquímica y Tecnología de Alimentos (IATA), Valencia, SpainIn this study optimization of the enzymatic hydrolysis condition of bee pollen protein by pepsin and the effect of enzymatic hydrolysis on its antioxidant properties was investigated. Also, the results were compared with antioxidant properties of royal jelly. For this purpose, phenolic compounds, DPPH free radical scavenging activity and ferric ion reducing power of bee pollen and royal jelly were measured. The values of these factors for bee pollen and royal jelly (1000 ppm), were 174 and 1031.71 (mg Gallic acid/ g sample), 67.33% and 95.27% and absorbance of 0.77 and 0.8 at 700 nm, respectively. The highest reducing power of 0.81 was measured in samples which were hydrolyzed by 2% pepsin for 4 h. Also, the highest DPPH radicals scavenging power was 100% which was achieved in hydrolyzed samples by 2% pepsin for 2.5 h. The highest ACE inhibitory activity was 91.49% in hydrolyzed samples by 2% pepsine for 4 h.  The results showed that the DPPH radical scavenging power of bee pollen increased from 67.33% to 100% and ACE inhibitory activity increased from 15.54% to 91.49% after enzymatic hydrolysis. The DPPH radical scavenging power and ACE inhibitory activity of bee pollen hydrolysats were comparable to those of royal jelly.http://journals.rifst.ac.ir/article_67046_2b7c14c388ac84a2047fe8e96f50766e.pdfaceantioxidant activitybee pollenenzymatic hydrolysisroyal jelly
collection DOAJ
language fas
format Article
sources DOAJ
author Atefe Maqsoudlou
Alireza Sadeghi Mahoonak
Mohammad Ghorbani
Fidel Toldra
spellingShingle Atefe Maqsoudlou
Alireza Sadeghi Mahoonak
Mohammad Ghorbani
Fidel Toldra
Optimization of Enzymatic Hydrolysis of Bee Pollen Protein by Pepsin Based on Antioxidant and ACE Inhibitory Activity and Comparison with Those of Royal Jelly
Pizhūhish va Nuāvarī dar ̒Ulūm va Sanāyi̒-i Ghaz̠āyī
ace
antioxidant activity
bee pollen
enzymatic hydrolysis
royal jelly
author_facet Atefe Maqsoudlou
Alireza Sadeghi Mahoonak
Mohammad Ghorbani
Fidel Toldra
author_sort Atefe Maqsoudlou
title Optimization of Enzymatic Hydrolysis of Bee Pollen Protein by Pepsin Based on Antioxidant and ACE Inhibitory Activity and Comparison with Those of Royal Jelly
title_short Optimization of Enzymatic Hydrolysis of Bee Pollen Protein by Pepsin Based on Antioxidant and ACE Inhibitory Activity and Comparison with Those of Royal Jelly
title_full Optimization of Enzymatic Hydrolysis of Bee Pollen Protein by Pepsin Based on Antioxidant and ACE Inhibitory Activity and Comparison with Those of Royal Jelly
title_fullStr Optimization of Enzymatic Hydrolysis of Bee Pollen Protein by Pepsin Based on Antioxidant and ACE Inhibitory Activity and Comparison with Those of Royal Jelly
title_full_unstemmed Optimization of Enzymatic Hydrolysis of Bee Pollen Protein by Pepsin Based on Antioxidant and ACE Inhibitory Activity and Comparison with Those of Royal Jelly
title_sort optimization of enzymatic hydrolysis of bee pollen protein by pepsin based on antioxidant and ace inhibitory activity and comparison with those of royal jelly
publisher Research Institute of Food Science and Technology
series Pizhūhish va Nuāvarī dar ̒Ulūm va Sanāyi̒-i Ghaz̠āyī
issn 2252-0937
2538-2357
publishDate 2018-05-01
description In this study optimization of the enzymatic hydrolysis condition of bee pollen protein by pepsin and the effect of enzymatic hydrolysis on its antioxidant properties was investigated. Also, the results were compared with antioxidant properties of royal jelly. For this purpose, phenolic compounds, DPPH free radical scavenging activity and ferric ion reducing power of bee pollen and royal jelly were measured. The values of these factors for bee pollen and royal jelly (1000 ppm), were 174 and 1031.71 (mg Gallic acid/ g sample), 67.33% and 95.27% and absorbance of 0.77 and 0.8 at 700 nm, respectively. The highest reducing power of 0.81 was measured in samples which were hydrolyzed by 2% pepsin for 4 h. Also, the highest DPPH radicals scavenging power was 100% which was achieved in hydrolyzed samples by 2% pepsin for 2.5 h. The highest ACE inhibitory activity was 91.49% in hydrolyzed samples by 2% pepsine for 4 h.  The results showed that the DPPH radical scavenging power of bee pollen increased from 67.33% to 100% and ACE inhibitory activity increased from 15.54% to 91.49% after enzymatic hydrolysis. The DPPH radical scavenging power and ACE inhibitory activity of bee pollen hydrolysats were comparable to those of royal jelly.
topic ace
antioxidant activity
bee pollen
enzymatic hydrolysis
royal jelly
url http://journals.rifst.ac.ir/article_67046_2b7c14c388ac84a2047fe8e96f50766e.pdf
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