Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq

Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq

Among four crude oil-degrading fungi strains that were isolated from a petroleum-polluted area in the Rumaila oil field, two fungi strains showed high activity in aliphatic hydrocarbon degradation. ITS sequencing and analysis of morphological and biochemical characteristics identified these strains...

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Main Authors: Adnan B. Al-Hawash, Jawadayn T. Alkooranee, Hayder A. Abbood, Jialong Zhang, Jin Sun, Xiaoyu Zhang, Fuying Ma
Format: Article
Language:English
Published: Elsevier 2018-03-01
Series:Biotechnology Reports
Subjects:
Biodegradation
Kinetics
Crude oil
2, 6-dichlorophenol indophenol (DCPIP)
Penicillium
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X1730303X
id doaj-685eab27af95426ea9d27067e833ab80
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spelling doaj-685eab27af95426ea9d27067e833ab802020-11-24T22:26:54ZengElsevierBiotechnology Reports2215-017X2018-03-0117C10410910.1016/j.btre.2017.12.006Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, IraqAdnan B. Al-Hawash0Jawadayn T. Alkooranee1Hayder A. Abbood2Jialong Zhang3Jin Sun4Xiaoyu Zhang5Fuying Ma6Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, ChinaKey Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, ChinaMaterial Engineering, College of Engineering, University of Basrah, IraqKey Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, ChinaKey Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, ChinaKey Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, ChinaKey Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, ChinaAmong four crude oil-degrading fungi strains that were isolated from a petroleum-polluted area in the Rumaila oil field, two fungi strains showed high activity in aliphatic hydrocarbon degradation. ITS sequencing and analysis of morphological and biochemical characteristics identified these strains as Penicillium sp. RMA1 and RMA2. Gravimetric and gas chromatography analysis of the crude oil remaining in the culture medium after 14 days of incubation at 30 °C showed that RMA1 and RMA2 degraded the crude oil by 57% and 55%, respectively. These strains reduced surface tension when cultured on crude oil (1% v/v) and exhibited a cell surface hydrophobicity of more than 70%. These results suggested that RMA1 and RMA2 performed effective crude oil-degrading activity and crude oil emulsification. In conclusion, these fungal strains can be used in bioremediation process and oil pollution reduction in aquatic ecosystems.http://www.sciencedirect.com/science/article/pii/S2215017X1730303XBiodegradationKineticsCrude oil2, 6-dichlorophenol indophenol (DCPIP)Penicillium
collection DOAJ
language English
format Article
sources DOAJ
author Adnan B. Al-Hawash
Jawadayn T. Alkooranee
Hayder A. Abbood
Jialong Zhang
Jin Sun
Xiaoyu Zhang
Fuying Ma
spellingShingle Adnan B. Al-Hawash
Jawadayn T. Alkooranee
Hayder A. Abbood
Jialong Zhang
Jin Sun
Xiaoyu Zhang
Fuying Ma
Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq
Biotechnology Reports
Biodegradation
Kinetics
Crude oil
2, 6-dichlorophenol indophenol (DCPIP)
Penicillium
author_facet Adnan B. Al-Hawash
Jawadayn T. Alkooranee
Hayder A. Abbood
Jialong Zhang
Jin Sun
Xiaoyu Zhang
Fuying Ma
author_sort Adnan B. Al-Hawash
title Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq
title_short Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq
title_full Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq
title_fullStr Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq
title_full_unstemmed Isolation and characterization of two crude oil-degrading fungi strains from Rumaila oil field, Iraq
title_sort isolation and characterization of two crude oil-degrading fungi strains from rumaila oil field, iraq
publisher Elsevier
series Biotechnology Reports
issn 2215-017X
publishDate 2018-03-01
description Among four crude oil-degrading fungi strains that were isolated from a petroleum-polluted area in the Rumaila oil field, two fungi strains showed high activity in aliphatic hydrocarbon degradation. ITS sequencing and analysis of morphological and biochemical characteristics identified these strains as Penicillium sp. RMA1 and RMA2. Gravimetric and gas chromatography analysis of the crude oil remaining in the culture medium after 14 days of incubation at 30 °C showed that RMA1 and RMA2 degraded the crude oil by 57% and 55%, respectively. These strains reduced surface tension when cultured on crude oil (1% v/v) and exhibited a cell surface hydrophobicity of more than 70%. These results suggested that RMA1 and RMA2 performed effective crude oil-degrading activity and crude oil emulsification. In conclusion, these fungal strains can be used in bioremediation process and oil pollution reduction in aquatic ecosystems.
topic Biodegradation
Kinetics
Crude oil
2, 6-dichlorophenol indophenol (DCPIP)
Penicillium
url http://www.sciencedirect.com/science/article/pii/S2215017X1730303X
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AT jawadayntalkooranee isolationandcharacterizationoftwocrudeoildegradingfungistrainsfromrumailaoilfieldiraq
AT hayderaabbood isolationandcharacterizationoftwocrudeoildegradingfungistrainsfromrumailaoilfieldiraq
AT jialongzhang isolationandcharacterizationoftwocrudeoildegradingfungistrainsfromrumailaoilfieldiraq
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