A detached leaf assay for testing transient gene expression and gene editing in cowpea (Vigna unguiculata [L.] Walp.)

A detached leaf assay for testing transient gene expression and gene editing in cowpea (Vigna unguiculata [L.] Walp.)

Abstract Background The legume cowpea (Vigna unguiculata L.) is extensively grown in sub-Saharan Africa. Cowpea, like many legumes has proved recalcitrant to plant transformation. A rapid transient leaf assay was developed for testing gene expression and editing constructs prior to stable cowpea tra...

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Bibliographic Details
Main Authors: Martina Juranić, Dilrukshi S. K. Nagahatenna, Rigel Salinas-Gamboa, Melanie L. Hand, Nidia Sánchez-León, Weng Herng Leong, Tracy How, Natalia Bazanova, Andrew Spriggs, Jean-Philippe Vielle-Calzada, Anna M. G. Koltunow
Format: Article
Language:English
Published: BMC 2020-06-01
Series:Plant Methods
Subjects:
Genome editing
CRISPR/Cas9
Cowpea
Meiosis
Leaf
Transient assay
Online Access:http://link.springer.com/article/10.1186/s13007-020-00630-4

Internet

http://link.springer.com/article/10.1186/s13007-020-00630-4

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