Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons
Store-operated calcium entry (SOCE) is a Calcium (Ca2+) influx pathway activated by depletion of intracellular stores that occurs in eukaryotic cells. In neurons, the presence and functions of SOCE are still in question. Here, we show evidences for the existence of SOCE in primary mouse cortical neu...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2017-12-01
|
Series: | Frontiers in Cellular Neuroscience |
Subjects: | |
Online Access: | http://journal.frontiersin.org/article/10.3389/fncel.2017.00363/full |
id |
doaj-68754b1fb97147c88ecfa91b8f846c1e |
---|---|
record_format |
Article |
spelling |
doaj-68754b1fb97147c88ecfa91b8f846c1e2020-11-25T00:06:22ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022017-12-011110.3389/fncel.2017.00363308122Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical NeuronsPaloma González-Sánchez0Paloma González-Sánchez1Paloma González-Sánchez2Araceli del Arco3Araceli del Arco4Araceli del Arco5José A. Esteban6Jorgina Satrústegui7Jorgina Satrústegui8Jorgina Satrústegui9Department of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), Madrid, SpainCentro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid, SpainInstituto de Investigación Sanitaria Fundación Jiménez Díaz (IIS-FJD), Madrid, SpainCentro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid, SpainInstituto de Investigación Sanitaria Fundación Jiménez Díaz (IIS-FJD), Madrid, SpainFacultad de Ciencias Ambientales y Bioquímica, Universidad de Castilla la Mancha, Toledo, SpainDepartment of Molecular Neurobiology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), Madrid, SpainDepartment of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), Madrid, SpainCentro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid, SpainInstituto de Investigación Sanitaria Fundación Jiménez Díaz (IIS-FJD), Madrid, SpainStore-operated calcium entry (SOCE) is a Calcium (Ca2+) influx pathway activated by depletion of intracellular stores that occurs in eukaryotic cells. In neurons, the presence and functions of SOCE are still in question. Here, we show evidences for the existence of SOCE in primary mouse cortical neurons. Endoplasmic reticulum (ER)-Ca2+ depletion using thapsigargin (Tg) triggered a maintained cytosolic Ca2+ increase, which rapidly returned to basal level in the presence of the SOCE blockers 2-Aminoethoxydiphenyl borate (2-APB) and YM-58483. Neural SOCE is also engaged by activation of metabotropic glutamate receptors (mGluRs) with (S)-3,5-dihydroxyphenylglycine (DHPG) (agonist of group I mGluRs), being an essential mechanism to maintain the mGluR-driven Ca2+ signal. Activation of group I of mGluRs triggers long-term depression (LTD) in many brain regions, but the underlying mechanism and, specifically, the necessity of Ca2+ increase in the postsynaptic neuron is controversial. In primary cortical neurons, we now show that the inhibition of Ca2+ influx through SOCE impaired DHPG-LTD, pointing out a key function of calcium and SOCE in synaptic plasticity.http://journal.frontiersin.org/article/10.3389/fncel.2017.00363/fullstore-operated calcium entry (SOCE)metabotropic glutamate receptors (mGluRs)long-term depression (LTD)calcium signalingsynaptic plasticity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paloma González-Sánchez Paloma González-Sánchez Paloma González-Sánchez Araceli del Arco Araceli del Arco Araceli del Arco José A. Esteban Jorgina Satrústegui Jorgina Satrústegui Jorgina Satrústegui |
spellingShingle |
Paloma González-Sánchez Paloma González-Sánchez Paloma González-Sánchez Araceli del Arco Araceli del Arco Araceli del Arco José A. Esteban Jorgina Satrústegui Jorgina Satrústegui Jorgina Satrústegui Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons Frontiers in Cellular Neuroscience store-operated calcium entry (SOCE) metabotropic glutamate receptors (mGluRs) long-term depression (LTD) calcium signaling synaptic plasticity |
author_facet |
Paloma González-Sánchez Paloma González-Sánchez Paloma González-Sánchez Araceli del Arco Araceli del Arco Araceli del Arco José A. Esteban Jorgina Satrústegui Jorgina Satrústegui Jorgina Satrústegui |
author_sort |
Paloma González-Sánchez |
title |
Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons |
title_short |
Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons |
title_full |
Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons |
title_fullStr |
Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons |
title_full_unstemmed |
Store-Operated Calcium Entry Is Required for mGluR-Dependent Long Term Depression in Cortical Neurons |
title_sort |
store-operated calcium entry is required for mglur-dependent long term depression in cortical neurons |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular Neuroscience |
issn |
1662-5102 |
publishDate |
2017-12-01 |
description |
Store-operated calcium entry (SOCE) is a Calcium (Ca2+) influx pathway activated by depletion of intracellular stores that occurs in eukaryotic cells. In neurons, the presence and functions of SOCE are still in question. Here, we show evidences for the existence of SOCE in primary mouse cortical neurons. Endoplasmic reticulum (ER)-Ca2+ depletion using thapsigargin (Tg) triggered a maintained cytosolic Ca2+ increase, which rapidly returned to basal level in the presence of the SOCE blockers 2-Aminoethoxydiphenyl borate (2-APB) and YM-58483. Neural SOCE is also engaged by activation of metabotropic glutamate receptors (mGluRs) with (S)-3,5-dihydroxyphenylglycine (DHPG) (agonist of group I mGluRs), being an essential mechanism to maintain the mGluR-driven Ca2+ signal. Activation of group I of mGluRs triggers long-term depression (LTD) in many brain regions, but the underlying mechanism and, specifically, the necessity of Ca2+ increase in the postsynaptic neuron is controversial. In primary cortical neurons, we now show that the inhibition of Ca2+ influx through SOCE impaired DHPG-LTD, pointing out a key function of calcium and SOCE in synaptic plasticity. |
topic |
store-operated calcium entry (SOCE) metabotropic glutamate receptors (mGluRs) long-term depression (LTD) calcium signaling synaptic plasticity |
url |
http://journal.frontiersin.org/article/10.3389/fncel.2017.00363/full |
work_keys_str_mv |
AT palomagonzalezsanchez storeoperatedcalciumentryisrequiredformglurdependentlongtermdepressionincorticalneurons AT palomagonzalezsanchez storeoperatedcalciumentryisrequiredformglurdependentlongtermdepressionincorticalneurons AT palomagonzalezsanchez storeoperatedcalciumentryisrequiredformglurdependentlongtermdepressionincorticalneurons AT aracelidelarco storeoperatedcalciumentryisrequiredformglurdependentlongtermdepressionincorticalneurons AT aracelidelarco storeoperatedcalciumentryisrequiredformglurdependentlongtermdepressionincorticalneurons AT aracelidelarco storeoperatedcalciumentryisrequiredformglurdependentlongtermdepressionincorticalneurons AT joseaesteban storeoperatedcalciumentryisrequiredformglurdependentlongtermdepressionincorticalneurons AT jorginasatrustegui storeoperatedcalciumentryisrequiredformglurdependentlongtermdepressionincorticalneurons AT jorginasatrustegui storeoperatedcalciumentryisrequiredformglurdependentlongtermdepressionincorticalneurons AT jorginasatrustegui storeoperatedcalciumentryisrequiredformglurdependentlongtermdepressionincorticalneurons |
_version_ |
1725422349651542016 |