Metabolic Profile of <i>Scytalidium parasiticum</i>-<i>Ganoderma boninense</i> Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity
In solving the issue of basal stem rot diseases caused by Ganoderma, an investigation of <i>Scytalidium parasiticum</i> as a biological control agent that suppresses Ganoderma infection has gained our interest, as it is more environmentally friendly. Recently, the fungal co-cultivation h...
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doaj-6876903f1d66474f86268131c3d005512020-12-17T00:04:48ZengMDPI AGMolecules1420-30492020-12-01255965596510.3390/molecules25245965Metabolic Profile of <i>Scytalidium parasiticum</i>-<i>Ganoderma boninense</i> Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma ActivityRafidah Ahmad0Choon Kiat Lim1Nurul Fadhilah Marzuki2Yit-Kheng Goh3Kamalrul Azlan Azizan4You Keng Goh5Kah Joo Goh6Ahmad Bazli Ramzi7Syarul Nataqain Baharum8Metabolomics Research Laboratory, Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, UKM, Bangi, Selangor 43600, MalaysiaAdvanced Agriecological Research Sdn Bhd, No. 11 Jalan Teknologi 3/6, Taman Sains Selangor 1, Kota Damansara, Petaling Jaya, Selangor Darul Ehsan 47810, MalaysiaAdvanced Agriecological Research Sdn Bhd, No. 11 Jalan Teknologi 3/6, Taman Sains Selangor 1, Kota Damansara, Petaling Jaya, Selangor Darul Ehsan 47810, MalaysiaAdvanced Agriecological Research Sdn Bhd, No. 11 Jalan Teknologi 3/6, Taman Sains Selangor 1, Kota Damansara, Petaling Jaya, Selangor Darul Ehsan 47810, MalaysiaMetabolomics Research Laboratory, Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, UKM, Bangi, Selangor 43600, MalaysiaAdvanced Agriecological Research Sdn Bhd, No. 11 Jalan Teknologi 3/6, Taman Sains Selangor 1, Kota Damansara, Petaling Jaya, Selangor Darul Ehsan 47810, MalaysiaAdvanced Agriecological Research Sdn Bhd, No. 11 Jalan Teknologi 3/6, Taman Sains Selangor 1, Kota Damansara, Petaling Jaya, Selangor Darul Ehsan 47810, MalaysiaMetabolomics Research Laboratory, Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, UKM, Bangi, Selangor 43600, MalaysiaMetabolomics Research Laboratory, Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, UKM, Bangi, Selangor 43600, MalaysiaIn solving the issue of basal stem rot diseases caused by Ganoderma, an investigation of <i>Scytalidium parasiticum</i> as a biological control agent that suppresses Ganoderma infection has gained our interest, as it is more environmentally friendly. Recently, the fungal co-cultivation has emerged as a promising method to discover novel antimicrobial metabolites. In this study, an established technique of co-culturing <i>Scytalidium parasiticum</i> and <i>Ganoderma boninense</i> was applied to produce and induce metabolites that have antifungal activity against <i>G. boninense</i>. The crude extract from the co-culture media was applied to a High Performance Liquid Chromatography (HPLC) preparative column to isolate the bioactive compounds, which were tested against <i>G. boninense</i>. The fractions that showed inhibition against <i>G. boninense</i> were sent for a Liquid Chromatography-Time of Flight-Mass Spectrometry (LC-TOF-MS) analysis to further identify the compounds that were responsible for the microbicidal activity. Interestingly, we found that eudistomin I, naringenin 7-O-beta-D-glucoside and penipanoid A, which were present in different abundances in all the active fractions, except in the control, could be the antimicrobial metabolites. In addition, the abundance of fatty acids, such as oleic acid and stearamide in the active fraction, also enhanced the antimicrobial activity. This comprehensive metabolomics study could be used as the basis for isolating biocontrol compounds to be applied in oil palm fields to combat a <i>Ganoderma</i> infection.https://www.mdpi.com/1420-3049/25/24/5965anti-Ganodermametabolomics<i>Scytalidium parasiticum</i><i>Ganoderma boninense</i>LC-TOF-MS analysisbiological control |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rafidah Ahmad Choon Kiat Lim Nurul Fadhilah Marzuki Yit-Kheng Goh Kamalrul Azlan Azizan You Keng Goh Kah Joo Goh Ahmad Bazli Ramzi Syarul Nataqain Baharum |
spellingShingle |
Rafidah Ahmad Choon Kiat Lim Nurul Fadhilah Marzuki Yit-Kheng Goh Kamalrul Azlan Azizan You Keng Goh Kah Joo Goh Ahmad Bazli Ramzi Syarul Nataqain Baharum Metabolic Profile of <i>Scytalidium parasiticum</i>-<i>Ganoderma boninense</i> Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity Molecules anti-Ganoderma metabolomics <i>Scytalidium parasiticum</i> <i>Ganoderma boninense</i> LC-TOF-MS analysis biological control |
author_facet |
Rafidah Ahmad Choon Kiat Lim Nurul Fadhilah Marzuki Yit-Kheng Goh Kamalrul Azlan Azizan You Keng Goh Kah Joo Goh Ahmad Bazli Ramzi Syarul Nataqain Baharum |
author_sort |
Rafidah Ahmad |
title |
Metabolic Profile of <i>Scytalidium parasiticum</i>-<i>Ganoderma boninense</i> Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity |
title_short |
Metabolic Profile of <i>Scytalidium parasiticum</i>-<i>Ganoderma boninense</i> Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity |
title_full |
Metabolic Profile of <i>Scytalidium parasiticum</i>-<i>Ganoderma boninense</i> Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity |
title_fullStr |
Metabolic Profile of <i>Scytalidium parasiticum</i>-<i>Ganoderma boninense</i> Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity |
title_full_unstemmed |
Metabolic Profile of <i>Scytalidium parasiticum</i>-<i>Ganoderma boninense</i> Co-Cultures Revealed the Alkaloids, Flavonoids and Fatty Acids that Contribute to Anti-Ganoderma Activity |
title_sort |
metabolic profile of <i>scytalidium parasiticum</i>-<i>ganoderma boninense</i> co-cultures revealed the alkaloids, flavonoids and fatty acids that contribute to anti-ganoderma activity |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-12-01 |
description |
In solving the issue of basal stem rot diseases caused by Ganoderma, an investigation of <i>Scytalidium parasiticum</i> as a biological control agent that suppresses Ganoderma infection has gained our interest, as it is more environmentally friendly. Recently, the fungal co-cultivation has emerged as a promising method to discover novel antimicrobial metabolites. In this study, an established technique of co-culturing <i>Scytalidium parasiticum</i> and <i>Ganoderma boninense</i> was applied to produce and induce metabolites that have antifungal activity against <i>G. boninense</i>. The crude extract from the co-culture media was applied to a High Performance Liquid Chromatography (HPLC) preparative column to isolate the bioactive compounds, which were tested against <i>G. boninense</i>. The fractions that showed inhibition against <i>G. boninense</i> were sent for a Liquid Chromatography-Time of Flight-Mass Spectrometry (LC-TOF-MS) analysis to further identify the compounds that were responsible for the microbicidal activity. Interestingly, we found that eudistomin I, naringenin 7-O-beta-D-glucoside and penipanoid A, which were present in different abundances in all the active fractions, except in the control, could be the antimicrobial metabolites. In addition, the abundance of fatty acids, such as oleic acid and stearamide in the active fraction, also enhanced the antimicrobial activity. This comprehensive metabolomics study could be used as the basis for isolating biocontrol compounds to be applied in oil palm fields to combat a <i>Ganoderma</i> infection. |
topic |
anti-Ganoderma metabolomics <i>Scytalidium parasiticum</i> <i>Ganoderma boninense</i> LC-TOF-MS analysis biological control |
url |
https://www.mdpi.com/1420-3049/25/24/5965 |
work_keys_str_mv |
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