Adamantane Containing Peptidoglycan Fragments Enhance RANTES and IL-6 Production in Lipopolysaccharide-Induced Macrophages
We report the enhancement of the lipopolysaccharide-induced immune response by adamantane containing peptidoglycan fragments in vitro. The immune stimulation was detected by Il-6 (interleukine 6) and RANTES (regulated on activation, normal T cell expressed and secreted) chemokine expression using ce...
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doaj-6882e42b1cd04aef851763dd6f3c27e52020-11-25T03:03:33ZengMDPI AGMolecules1420-30492020-08-01253707370710.3390/molecules25163707Adamantane Containing Peptidoglycan Fragments Enhance RANTES and IL-6 Production in Lipopolysaccharide-Induced MacrophagesMateja Manček-Keber0Rosana Ribić1Fernando Chain2Davy Sinnaeve3José C. Martins4Roman Jerala5Srđanka Tomić6Krisztina Fehér7Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, P.O. Box 660, SI-1001 Ljubljana, SloveniaUniversity Center Varaždin, University North, Jurja Križanića 31b, HR-42 000 Varaždin, CroatiaDepartment of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281 S4, 9000 Ghent, BelgiumDepartment of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281 S4, 9000 Ghent, BelgiumDepartment of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281 S4, 9000 Ghent, BelgiumDepartment of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, P.O. Box 660, SI-1001 Ljubljana, SloveniaDepartment of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102A, HR-10 000 Zagreb, CroatiaDepartment of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102A, HR-10 000 Zagreb, CroatiaWe report the enhancement of the lipopolysaccharide-induced immune response by adamantane containing peptidoglycan fragments in vitro. The immune stimulation was detected by Il-6 (interleukine 6) and RANTES (regulated on activation, normal T cell expressed and secreted) chemokine expression using cell assays on immortalized mouse bone-marrow derived macrophages. The most active compound was a α-D-mannosyl derivative of an adamantylated tripeptide with L-chirality at the adamantyl group attachment, whereby the mannose moiety assumed to target mannose receptors expressed on macrophage cell surfaces. The immune co-stimulatory effect was also influenced by the configuration of the adamantyl center, revealing the importance of specific molecular recognition event taking place with its receptor. The immunostimulating activities of these compounds were further enhanced upon their incorporation into lipid bilayers, which is likely related to the presence of the adamantyl group that helps anchor the peptidoglycan fragment into lipid nanoparticles. We concluded that the proposed adamantane containing peptidoglycan fragments act as co-stimulatory agents and are also suitable for the preparation of lipid nanoparticle-based delivery of peptidoglycan fragments.https://www.mdpi.com/1420-3049/25/16/3707peptidoglycanimmunostimulationlipid incapsulationadamantanemannose |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mateja Manček-Keber Rosana Ribić Fernando Chain Davy Sinnaeve José C. Martins Roman Jerala Srđanka Tomić Krisztina Fehér |
spellingShingle |
Mateja Manček-Keber Rosana Ribić Fernando Chain Davy Sinnaeve José C. Martins Roman Jerala Srđanka Tomić Krisztina Fehér Adamantane Containing Peptidoglycan Fragments Enhance RANTES and IL-6 Production in Lipopolysaccharide-Induced Macrophages Molecules peptidoglycan immunostimulation lipid incapsulation adamantane mannose |
author_facet |
Mateja Manček-Keber Rosana Ribić Fernando Chain Davy Sinnaeve José C. Martins Roman Jerala Srđanka Tomić Krisztina Fehér |
author_sort |
Mateja Manček-Keber |
title |
Adamantane Containing Peptidoglycan Fragments Enhance RANTES and IL-6 Production in Lipopolysaccharide-Induced Macrophages |
title_short |
Adamantane Containing Peptidoglycan Fragments Enhance RANTES and IL-6 Production in Lipopolysaccharide-Induced Macrophages |
title_full |
Adamantane Containing Peptidoglycan Fragments Enhance RANTES and IL-6 Production in Lipopolysaccharide-Induced Macrophages |
title_fullStr |
Adamantane Containing Peptidoglycan Fragments Enhance RANTES and IL-6 Production in Lipopolysaccharide-Induced Macrophages |
title_full_unstemmed |
Adamantane Containing Peptidoglycan Fragments Enhance RANTES and IL-6 Production in Lipopolysaccharide-Induced Macrophages |
title_sort |
adamantane containing peptidoglycan fragments enhance rantes and il-6 production in lipopolysaccharide-induced macrophages |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-08-01 |
description |
We report the enhancement of the lipopolysaccharide-induced immune response by adamantane containing peptidoglycan fragments in vitro. The immune stimulation was detected by Il-6 (interleukine 6) and RANTES (regulated on activation, normal T cell expressed and secreted) chemokine expression using cell assays on immortalized mouse bone-marrow derived macrophages. The most active compound was a α-D-mannosyl derivative of an adamantylated tripeptide with L-chirality at the adamantyl group attachment, whereby the mannose moiety assumed to target mannose receptors expressed on macrophage cell surfaces. The immune co-stimulatory effect was also influenced by the configuration of the adamantyl center, revealing the importance of specific molecular recognition event taking place with its receptor. The immunostimulating activities of these compounds were further enhanced upon their incorporation into lipid bilayers, which is likely related to the presence of the adamantyl group that helps anchor the peptidoglycan fragment into lipid nanoparticles. We concluded that the proposed adamantane containing peptidoglycan fragments act as co-stimulatory agents and are also suitable for the preparation of lipid nanoparticle-based delivery of peptidoglycan fragments. |
topic |
peptidoglycan immunostimulation lipid incapsulation adamantane mannose |
url |
https://www.mdpi.com/1420-3049/25/16/3707 |
work_keys_str_mv |
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