Identification of Novel Biomarker for Early Detection of Diabetic Nephropathy
Diabetic nephropathy (DN) is one of the most common complications of diabetes mellitus. After development of DN, patients will progress to end-stage renal disease, which is associated with high morbidity and mortality. Here, we developed early-stage diagnostic biomarkers to detect DN as a strategy f...
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doaj-688cee5892f849d7b0d12f2ed0fbe5382021-04-22T23:05:05ZengMDPI AGBiomedicines2227-90592021-04-01945745710.3390/biomedicines9050457Identification of Novel Biomarker for Early Detection of Diabetic NephropathyKyeong-Seok Kim0Jin-Sol Lee1Jae-Hyeon Park2Eun-Young Lee3Jong-Seok Moon4Sangkyu Lee5Jong-Sil Lee6Jung-Hwan Kim7Hyung-Sik Kim8School of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaDepartment of Internal Medicine, Soonchunhyang University Cheonan Hospital, Cheonan 31151, KoreaDepartment of Integrated Biomedical Science, Soonchunhyang Institute of Medi-Bio Science, Soonchunhyang University, Cheonan 31151, KoreaBK21 Plus KNU Multi‐Omics Based Creative Drug Research Team, College of Pharmacy, Kyungpook National University, Daegu 41566, KoreaDepartment of Pathology, Institute of Health Sciences, College of Medicine, Gyeongsang National University Hospital, Jinju 52727, KoreaDepartment of Pharmacology, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52727, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaDiabetic nephropathy (DN) is one of the most common complications of diabetes mellitus. After development of DN, patients will progress to end-stage renal disease, which is associated with high morbidity and mortality. Here, we developed early-stage diagnostic biomarkers to detect DN as a strategy for DN intervention. For the DN model, Zucker diabetic fatty rats were used for DN phenotyping. The results revealed that DN rats showed significantly increased blood glucose, blood urea nitrogen (BUN), and serum creatinine levels, accompanied by severe kidney injury, fibrosis and microstructural changes. In addition, DN rats showed significantly increased urinary excretion of kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL). Transcriptome analysis revealed that new DN biomarkers, such as complementary component 4b (C4b), complementary factor D (CFD), C-X-C motif chemokine receptor 6 (CXCR6), and leukemia inhibitory factor (LIF) were identified. Furthermore, they were found in the urine of patients with DN. Since these biomarkers were detected in the urine and kidney of DN rats and urine of diabetic patients, the selected markers could be used as early diagnosis biomarkers for chronic diabetic nephropathy.https://www.mdpi.com/2227-9059/9/5/457RNA-sequencingbiomarkerurineCFDCXCR6C4b |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kyeong-Seok Kim Jin-Sol Lee Jae-Hyeon Park Eun-Young Lee Jong-Seok Moon Sangkyu Lee Jong-Sil Lee Jung-Hwan Kim Hyung-Sik Kim |
spellingShingle |
Kyeong-Seok Kim Jin-Sol Lee Jae-Hyeon Park Eun-Young Lee Jong-Seok Moon Sangkyu Lee Jong-Sil Lee Jung-Hwan Kim Hyung-Sik Kim Identification of Novel Biomarker for Early Detection of Diabetic Nephropathy Biomedicines RNA-sequencing biomarker urine CFD CXCR6 C4b |
author_facet |
Kyeong-Seok Kim Jin-Sol Lee Jae-Hyeon Park Eun-Young Lee Jong-Seok Moon Sangkyu Lee Jong-Sil Lee Jung-Hwan Kim Hyung-Sik Kim |
author_sort |
Kyeong-Seok Kim |
title |
Identification of Novel Biomarker for Early Detection of Diabetic Nephropathy |
title_short |
Identification of Novel Biomarker for Early Detection of Diabetic Nephropathy |
title_full |
Identification of Novel Biomarker for Early Detection of Diabetic Nephropathy |
title_fullStr |
Identification of Novel Biomarker for Early Detection of Diabetic Nephropathy |
title_full_unstemmed |
Identification of Novel Biomarker for Early Detection of Diabetic Nephropathy |
title_sort |
identification of novel biomarker for early detection of diabetic nephropathy |
publisher |
MDPI AG |
series |
Biomedicines |
issn |
2227-9059 |
publishDate |
2021-04-01 |
description |
Diabetic nephropathy (DN) is one of the most common complications of diabetes mellitus. After development of DN, patients will progress to end-stage renal disease, which is associated with high morbidity and mortality. Here, we developed early-stage diagnostic biomarkers to detect DN as a strategy for DN intervention. For the DN model, Zucker diabetic fatty rats were used for DN phenotyping. The results revealed that DN rats showed significantly increased blood glucose, blood urea nitrogen (BUN), and serum creatinine levels, accompanied by severe kidney injury, fibrosis and microstructural changes. In addition, DN rats showed significantly increased urinary excretion of kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL). Transcriptome analysis revealed that new DN biomarkers, such as complementary component 4b (C4b), complementary factor D (CFD), C-X-C motif chemokine receptor 6 (CXCR6), and leukemia inhibitory factor (LIF) were identified. Furthermore, they were found in the urine of patients with DN. Since these biomarkers were detected in the urine and kidney of DN rats and urine of diabetic patients, the selected markers could be used as early diagnosis biomarkers for chronic diabetic nephropathy. |
topic |
RNA-sequencing biomarker urine CFD CXCR6 C4b |
url |
https://www.mdpi.com/2227-9059/9/5/457 |
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