Uptake of 18F-FET and 18F-FCH in Human Glioblastoma T98G Cell Line after Irradiation with Photons or Carbon Ions
The differential diagnosis between recurrence of gliomas or brain metastases and this phenomenon is important in order to choose the best therapy and predict the prognosis but is still a big problem for physicians. The new emerging MRI, CT, and PET diagnostic modalities still lack sufficient accurac...
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doaj-68a0a469c70c4cd5b214267c3f9bed8e2020-11-25T00:30:17ZengHindawi-WileyContrast Media & Molecular Imaging1555-43091555-43172017-01-01201710.1155/2017/64916746491674Uptake of 18F-FET and 18F-FCH in Human Glioblastoma T98G Cell Line after Irradiation with Photons or Carbon IonsFrancesca Pasi0Marco Giovanni Persico1Federica Eleonora Buroni2Carlo Aprile3Marina Hodolic4Franco Corbella5Rosanna Nano6Angelica Facoetti7Lorenzo Lodola8Department of Oncohaematology, Radiotherapy Unit, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, 27100 Pavia, ItalyDepartment of Oncohaematology, Nuclear Medicine Unit, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, 27100 Pavia, ItalyDepartment of Oncohaematology, Nuclear Medicine Unit, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, 27100 Pavia, ItalyDepartment of Oncohaematology, Nuclear Medicine Unit, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, 27100 Pavia, ItalyNuclear Medicine Research Department, IASON GmbH, Graz, AustriaDepartment of Oncohaematology, Radiotherapy Unit, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, 27100 Pavia, ItalyDepartment of Biology and Biotechnology “Lazzaro Spallanzani”, University of Pavia, Via Ferrata 9, 27100 Pavia, ItalyNational Centre for Oncological Hadrontherapy (CNAO), Via Campeggi 53, 27100 Pavia, ItalyDepartment of Oncohaematology, Nuclear Medicine Unit, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, 27100 Pavia, ItalyThe differential diagnosis between recurrence of gliomas or brain metastases and this phenomenon is important in order to choose the best therapy and predict the prognosis but is still a big problem for physicians. The new emerging MRI, CT, and PET diagnostic modalities still lack sufficient accuracy. Radiolabeled choline and amino acids have been reported to show great tumor specificity. We studied the uptake kinetics of [18F]fluoromethyl-choline (FCH) and O-(2-[18F]fluoroethyl)-L-tyrosine (FET) by the T98G human glioblastoma cells from 20 to 120 min after irradiation either with photons at 2-10-20 Gy or with carbon ions at 2 Gy (at the National Centre for Oncological Hadrontherapy (CNAO), Pavia, Italy). We also evaluated the cell death and morphology changes induced by radiation treatment. Both FET and FCH are able to trace tumor behavior in terms of higher uptake for increased doses of radiation treatment, due to the upregulation of cells attempts to repair nonlethal damage. Our data suggest that both FCH and FET could be useful to analyze the metabolic pathways of glioblastoma cells before and after radiotherapy. Physicians will have to consider the different kinetics pathways of uptake concerning the two radiopharmaceuticals.http://dx.doi.org/10.1155/2017/6491674 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Francesca Pasi Marco Giovanni Persico Federica Eleonora Buroni Carlo Aprile Marina Hodolic Franco Corbella Rosanna Nano Angelica Facoetti Lorenzo Lodola |
spellingShingle |
Francesca Pasi Marco Giovanni Persico Federica Eleonora Buroni Carlo Aprile Marina Hodolic Franco Corbella Rosanna Nano Angelica Facoetti Lorenzo Lodola Uptake of 18F-FET and 18F-FCH in Human Glioblastoma T98G Cell Line after Irradiation with Photons or Carbon Ions Contrast Media & Molecular Imaging |
author_facet |
Francesca Pasi Marco Giovanni Persico Federica Eleonora Buroni Carlo Aprile Marina Hodolic Franco Corbella Rosanna Nano Angelica Facoetti Lorenzo Lodola |
author_sort |
Francesca Pasi |
title |
Uptake of 18F-FET and 18F-FCH in Human Glioblastoma T98G Cell Line after Irradiation with Photons or Carbon Ions |
title_short |
Uptake of 18F-FET and 18F-FCH in Human Glioblastoma T98G Cell Line after Irradiation with Photons or Carbon Ions |
title_full |
Uptake of 18F-FET and 18F-FCH in Human Glioblastoma T98G Cell Line after Irradiation with Photons or Carbon Ions |
title_fullStr |
Uptake of 18F-FET and 18F-FCH in Human Glioblastoma T98G Cell Line after Irradiation with Photons or Carbon Ions |
title_full_unstemmed |
Uptake of 18F-FET and 18F-FCH in Human Glioblastoma T98G Cell Line after Irradiation with Photons or Carbon Ions |
title_sort |
uptake of 18f-fet and 18f-fch in human glioblastoma t98g cell line after irradiation with photons or carbon ions |
publisher |
Hindawi-Wiley |
series |
Contrast Media & Molecular Imaging |
issn |
1555-4309 1555-4317 |
publishDate |
2017-01-01 |
description |
The differential diagnosis between recurrence of gliomas or brain metastases and this phenomenon is important in order to choose the best therapy and predict the prognosis but is still a big problem for physicians. The new emerging MRI, CT, and PET diagnostic modalities still lack sufficient accuracy. Radiolabeled choline and amino acids have been reported to show great tumor specificity. We studied the uptake kinetics of [18F]fluoromethyl-choline (FCH) and O-(2-[18F]fluoroethyl)-L-tyrosine (FET) by the T98G human glioblastoma cells from 20 to 120 min after irradiation either with photons at 2-10-20 Gy or with carbon ions at 2 Gy (at the National Centre for Oncological Hadrontherapy (CNAO), Pavia, Italy). We also evaluated the cell death and morphology changes induced by radiation treatment. Both FET and FCH are able to trace tumor behavior in terms of higher uptake for increased doses of radiation treatment, due to the upregulation of cells attempts to repair nonlethal damage. Our data suggest that both FCH and FET could be useful to analyze the metabolic pathways of glioblastoma cells before and after radiotherapy. Physicians will have to consider the different kinetics pathways of uptake concerning the two radiopharmaceuticals. |
url |
http://dx.doi.org/10.1155/2017/6491674 |
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