Comparison of Humoral Immune Responses to Different Forms of Salmonella enterica Serovar Gallinarum Biovar Gallinarum

Fowl typhoid is caused by Salmonella enterica serovar Gallinarum biovar Gallinarum (SG), and live attenuated, rough vaccine strains have been used. Both humoral and cellular immune responses are involved in protection, but the humoral responses to different forms of SG antigens are unclear. In this...

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Main Authors: Nam-Hyung Kim, Eun-Jin Ha, Dae-Sung Ko, Kang-Seuk Choi, Hyuk-Joon Kwon
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-11-01
Series:Frontiers in Veterinary Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2020.598610/full
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spelling doaj-68abdb2c96af4b6aaa313e68d81c938c2020-11-25T04:05:58ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692020-11-01710.3389/fvets.2020.598610598610Comparison of Humoral Immune Responses to Different Forms of Salmonella enterica Serovar Gallinarum Biovar GallinarumNam-Hyung Kim0Nam-Hyung Kim1Eun-Jin Ha2Eun-Jin Ha3Dae-Sung Ko4Dae-Sung Ko5Kang-Seuk Choi6Kang-Seuk Choi7Hyuk-Joon Kwon8Hyuk-Joon Kwon9Hyuk-Joon Kwon10Laboratory of Poultry Medicine, Seoul National University, Seoul, South KoreaResearch Institute for Veterinary Science, College of Veterinary Medicine, BK21 for Veterinary Science, Seoul, South KoreaLaboratory of Poultry Medicine, Seoul National University, Seoul, South KoreaResearch Institute for Veterinary Science, College of Veterinary Medicine, BK21 for Veterinary Science, Seoul, South KoreaLaboratory of Poultry Medicine, Seoul National University, Seoul, South KoreaResearch Institute for Veterinary Science, College of Veterinary Medicine, BK21 for Veterinary Science, Seoul, South KoreaResearch Institute for Veterinary Science, College of Veterinary Medicine, BK21 for Veterinary Science, Seoul, South KoreaLaboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University, Seoul, South KoreaLaboratory of Poultry Medicine, Seoul National University, Seoul, South KoreaResearch Institute for Veterinary Science, College of Veterinary Medicine, BK21 for Veterinary Science, Seoul, South KoreaFarm Animal Clinical Training and Research Center (FACTRC), GBST, Seoul National University, Seoul, South KoreaFowl typhoid is caused by Salmonella enterica serovar Gallinarum biovar Gallinarum (SG), and live attenuated, rough vaccine strains have been used. Both humoral and cellular immune responses are involved in protection, but the humoral responses to different forms of SG antigens are unclear. In this study, we compared humoral responses to a killed oil-emulsion (OE) smooth vaccine (SG002) and its rough mutant vaccine (SR2-N6) strains using proteomics techniques. We identified two immunogenic outer membrane proteins (OmpA and OmpX), and the selected linear epitopes were successfully applied in peptide-ELISA. Our peptide- and total OMP-ELISAs were used to compare the temporal humoral responses to various SG antigens: OE SG002 and SR2-N6; live, killed [PBS-suspension (PS) and OE)] and mixed (live and PS) formulations of another rough vaccine strain (SG 9R); and orally challenge with a field strain. Serum antibodies to the linear epitopes of OmpA and OmpX lasted only for the first 2 weeks, but serum antibodies against OMPs increased over time. The rough strain (SR2-N6) and mixed SG 9R induced higher serum antibody titers than the smooth strain (SG002) and single SG 9R (OE, live and PS SG 9R), respectively. Infection with the field strain delayed the serum antibody response by ~2 weeks. Mucosal immunity was not induced by any formulation, except for infection with the field strain after SG 9R vaccination. Thus, our results may be useful to understand humoral immunity against various SG antigens and to improve vaccine programs and serological diagnosis in the field.https://www.frontiersin.org/articles/10.3389/fvets.2020.598610/fullSalmonella serovar Gallinarum biovar Gallinarumhumoral immunityvaccinesnatural infectionPeptide-ELISA
collection DOAJ
language English
format Article
sources DOAJ
author Nam-Hyung Kim
Nam-Hyung Kim
Eun-Jin Ha
Eun-Jin Ha
Dae-Sung Ko
Dae-Sung Ko
Kang-Seuk Choi
Kang-Seuk Choi
Hyuk-Joon Kwon
Hyuk-Joon Kwon
Hyuk-Joon Kwon
spellingShingle Nam-Hyung Kim
Nam-Hyung Kim
Eun-Jin Ha
Eun-Jin Ha
Dae-Sung Ko
Dae-Sung Ko
Kang-Seuk Choi
Kang-Seuk Choi
Hyuk-Joon Kwon
Hyuk-Joon Kwon
Hyuk-Joon Kwon
Comparison of Humoral Immune Responses to Different Forms of Salmonella enterica Serovar Gallinarum Biovar Gallinarum
Frontiers in Veterinary Science
Salmonella serovar Gallinarum biovar Gallinarum
humoral immunity
vaccines
natural infection
Peptide-ELISA
author_facet Nam-Hyung Kim
Nam-Hyung Kim
Eun-Jin Ha
Eun-Jin Ha
Dae-Sung Ko
Dae-Sung Ko
Kang-Seuk Choi
Kang-Seuk Choi
Hyuk-Joon Kwon
Hyuk-Joon Kwon
Hyuk-Joon Kwon
author_sort Nam-Hyung Kim
title Comparison of Humoral Immune Responses to Different Forms of Salmonella enterica Serovar Gallinarum Biovar Gallinarum
title_short Comparison of Humoral Immune Responses to Different Forms of Salmonella enterica Serovar Gallinarum Biovar Gallinarum
title_full Comparison of Humoral Immune Responses to Different Forms of Salmonella enterica Serovar Gallinarum Biovar Gallinarum
title_fullStr Comparison of Humoral Immune Responses to Different Forms of Salmonella enterica Serovar Gallinarum Biovar Gallinarum
title_full_unstemmed Comparison of Humoral Immune Responses to Different Forms of Salmonella enterica Serovar Gallinarum Biovar Gallinarum
title_sort comparison of humoral immune responses to different forms of salmonella enterica serovar gallinarum biovar gallinarum
publisher Frontiers Media S.A.
series Frontiers in Veterinary Science
issn 2297-1769
publishDate 2020-11-01
description Fowl typhoid is caused by Salmonella enterica serovar Gallinarum biovar Gallinarum (SG), and live attenuated, rough vaccine strains have been used. Both humoral and cellular immune responses are involved in protection, but the humoral responses to different forms of SG antigens are unclear. In this study, we compared humoral responses to a killed oil-emulsion (OE) smooth vaccine (SG002) and its rough mutant vaccine (SR2-N6) strains using proteomics techniques. We identified two immunogenic outer membrane proteins (OmpA and OmpX), and the selected linear epitopes were successfully applied in peptide-ELISA. Our peptide- and total OMP-ELISAs were used to compare the temporal humoral responses to various SG antigens: OE SG002 and SR2-N6; live, killed [PBS-suspension (PS) and OE)] and mixed (live and PS) formulations of another rough vaccine strain (SG 9R); and orally challenge with a field strain. Serum antibodies to the linear epitopes of OmpA and OmpX lasted only for the first 2 weeks, but serum antibodies against OMPs increased over time. The rough strain (SR2-N6) and mixed SG 9R induced higher serum antibody titers than the smooth strain (SG002) and single SG 9R (OE, live and PS SG 9R), respectively. Infection with the field strain delayed the serum antibody response by ~2 weeks. Mucosal immunity was not induced by any formulation, except for infection with the field strain after SG 9R vaccination. Thus, our results may be useful to understand humoral immunity against various SG antigens and to improve vaccine programs and serological diagnosis in the field.
topic Salmonella serovar Gallinarum biovar Gallinarum
humoral immunity
vaccines
natural infection
Peptide-ELISA
url https://www.frontiersin.org/articles/10.3389/fvets.2020.598610/full
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