Preparation and Characterization of an Antibody Antagonist That Targets the Porcine Growth Hormone Receptor
A series of antagonists specifically targeting growth hormone receptors (GHR) in different species, such as humans, rats, bovines, and mice, have been designed; however, there are currently no antagonists that target the porcine growth hormone (GH). Therefore, in this study, we developed and charact...
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Asian-Australasian Association of Animal Production Societies
2016-10-01
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doaj-68b1e06d1add4e3b98a4fc55cba8820c2020-11-24T23:58:13ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172016-10-0129101508151410.5713/ajas.15.089223440Preparation and Characterization of an Antibody Antagonist That Targets the Porcine Growth Hormone ReceptorHuanzhong CuiYanrong Wang0Meng SongHui Zhang Henan University of Science and Technology, Xinxiang, Henan 471023, ChinaA series of antagonists specifically targeting growth hormone receptors (GHR) in different species, such as humans, rats, bovines, and mice, have been designed; however, there are currently no antagonists that target the porcine growth hormone (GH). Therefore, in this study, we developed and characterized a porcine GHR (pGHR) antibody antagonist (denoted by AN98) via the hybridoma technique. The results from enzyme-linked immunosorbent assay, fluorescence activated cell sorter, indirect immunoinfluscent assay, and competitive receptor binding analysis showed that AN98 could specifically recognize pGHR, and further experiments indicated that AN98 could effectively inhibit pGH-induced signalling in CHO-pGHR cells and porcine hepatocytes. In addition, AN98 also inhibited GH-induced insulin-like growth factor-1 (IGF-1) secretion in porcine hepatocytes. In summary, these findings indicated that AN98, as a pGHR-specific antagonist, has potential applications in pGH-pGHR-related research on domestic pigs.http://www.ajas.info/upload/pdf/ajas-29-10-1508.pdfPorcine Growth HormonePorcine Growth Hormone ReceptorsSignalling PathwayAntagonist |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huanzhong Cui Yanrong Wang Meng Song Hui Zhang |
spellingShingle |
Huanzhong Cui Yanrong Wang Meng Song Hui Zhang Preparation and Characterization of an Antibody Antagonist That Targets the Porcine Growth Hormone Receptor Asian-Australasian Journal of Animal Sciences Porcine Growth Hormone Porcine Growth Hormone Receptors Signalling Pathway Antagonist |
author_facet |
Huanzhong Cui Yanrong Wang Meng Song Hui Zhang |
author_sort |
Huanzhong Cui |
title |
Preparation and Characterization of an Antibody Antagonist That Targets the Porcine Growth Hormone Receptor |
title_short |
Preparation and Characterization of an Antibody Antagonist That Targets the Porcine Growth Hormone Receptor |
title_full |
Preparation and Characterization of an Antibody Antagonist That Targets the Porcine Growth Hormone Receptor |
title_fullStr |
Preparation and Characterization of an Antibody Antagonist That Targets the Porcine Growth Hormone Receptor |
title_full_unstemmed |
Preparation and Characterization of an Antibody Antagonist That Targets the Porcine Growth Hormone Receptor |
title_sort |
preparation and characterization of an antibody antagonist that targets the porcine growth hormone receptor |
publisher |
Asian-Australasian Association of Animal Production Societies |
series |
Asian-Australasian Journal of Animal Sciences |
issn |
1011-2367 1976-5517 |
publishDate |
2016-10-01 |
description |
A series of antagonists specifically targeting growth hormone receptors (GHR) in different species, such as humans, rats, bovines, and mice, have been designed; however, there are currently no antagonists that target the porcine growth hormone (GH). Therefore, in this study, we developed and characterized a porcine GHR (pGHR) antibody antagonist (denoted by AN98) via the hybridoma technique. The results from enzyme-linked immunosorbent assay, fluorescence activated cell sorter, indirect immunoinfluscent assay, and competitive receptor binding analysis showed that AN98 could specifically recognize pGHR, and further experiments indicated that AN98 could effectively inhibit pGH-induced signalling in CHO-pGHR cells and porcine hepatocytes. In addition, AN98 also inhibited GH-induced insulin-like growth factor-1 (IGF-1) secretion in porcine hepatocytes. In summary, these findings indicated that AN98, as a pGHR-specific antagonist, has potential applications in pGH-pGHR-related research on domestic pigs. |
topic |
Porcine Growth Hormone Porcine Growth Hormone Receptors Signalling Pathway Antagonist |
url |
http://www.ajas.info/upload/pdf/ajas-29-10-1508.pdf |
work_keys_str_mv |
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