Diet-Induced Podocyte Dysfunction in Drosophila and Mammals
Diabetic nephropathy is a major cause of end-stage kidney disease. Characterized by progressive microvascular disease, most efforts have focused on injury to the glomerular endothelium. Recent work has suggested a role for the podocyte, a highly specialized component of the glomerular filtration bar...
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doaj-68c11c1b84394832bf065d51634730e52020-11-25T01:17:24ZengElsevierCell Reports2211-12472015-07-0112463664710.1016/j.celrep.2015.06.056Diet-Induced Podocyte Dysfunction in Drosophila and MammalsJianbo Na0Mariya T. Sweetwyne1Ae Seo Deok Park2Katalin Susztak3Ross L. Cagan4Department of Developmental and Regenerative Biology and School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, One Gustave Levy Place, New York, NY 10029-1020, USARenal Electrolyte and Hypertension Division, Perelman School of Medicine, University of Pennsylvania, 415 Curie Boulevard, 405B Clinical Research Building, Philadelphia, PA 19104-4539, USARenal Electrolyte and Hypertension Division, Perelman School of Medicine, University of Pennsylvania, 415 Curie Boulevard, 405B Clinical Research Building, Philadelphia, PA 19104-4539, USARenal Electrolyte and Hypertension Division, Perelman School of Medicine, University of Pennsylvania, 415 Curie Boulevard, 405B Clinical Research Building, Philadelphia, PA 19104-4539, USADepartment of Developmental and Regenerative Biology and School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, One Gustave Levy Place, New York, NY 10029-1020, USADiabetic nephropathy is a major cause of end-stage kidney disease. Characterized by progressive microvascular disease, most efforts have focused on injury to the glomerular endothelium. Recent work has suggested a role for the podocyte, a highly specialized component of the glomerular filtration barrier. Here, we demonstrate that the Drosophila nephrocyte, a cell analogous to the mammalian podocyte, displays defects that phenocopy aspects of diabetic nephropathy in animals fed chronic high dietary sucrose. Through functional studies, we identify an OGT-Polycomb-Knot-Sns pathway that links dietary sucrose to loss of the Nephrin ortholog Sns. Reducing OGT through genetic or drug means is sufficient to rescue loss of Sns, leading to overall extension of lifespan. We demonstrate upregulation of the Knot ortholog EBF2 in glomeruli of human diabetic nephropathy patients and a mouse ob/ob diabetes model. Furthermore, we demonstrate rescue of Nephrin expression and cell viability in ebf2−/− primary podocytes cultured in high glucose.http://www.sciencedirect.com/science/article/pii/S2211124715006828 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jianbo Na Mariya T. Sweetwyne Ae Seo Deok Park Katalin Susztak Ross L. Cagan |
spellingShingle |
Jianbo Na Mariya T. Sweetwyne Ae Seo Deok Park Katalin Susztak Ross L. Cagan Diet-Induced Podocyte Dysfunction in Drosophila and Mammals Cell Reports |
author_facet |
Jianbo Na Mariya T. Sweetwyne Ae Seo Deok Park Katalin Susztak Ross L. Cagan |
author_sort |
Jianbo Na |
title |
Diet-Induced Podocyte Dysfunction in Drosophila and Mammals |
title_short |
Diet-Induced Podocyte Dysfunction in Drosophila and Mammals |
title_full |
Diet-Induced Podocyte Dysfunction in Drosophila and Mammals |
title_fullStr |
Diet-Induced Podocyte Dysfunction in Drosophila and Mammals |
title_full_unstemmed |
Diet-Induced Podocyte Dysfunction in Drosophila and Mammals |
title_sort |
diet-induced podocyte dysfunction in drosophila and mammals |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2015-07-01 |
description |
Diabetic nephropathy is a major cause of end-stage kidney disease. Characterized by progressive microvascular disease, most efforts have focused on injury to the glomerular endothelium. Recent work has suggested a role for the podocyte, a highly specialized component of the glomerular filtration barrier. Here, we demonstrate that the Drosophila nephrocyte, a cell analogous to the mammalian podocyte, displays defects that phenocopy aspects of diabetic nephropathy in animals fed chronic high dietary sucrose. Through functional studies, we identify an OGT-Polycomb-Knot-Sns pathway that links dietary sucrose to loss of the Nephrin ortholog Sns. Reducing OGT through genetic or drug means is sufficient to rescue loss of Sns, leading to overall extension of lifespan. We demonstrate upregulation of the Knot ortholog EBF2 in glomeruli of human diabetic nephropathy patients and a mouse ob/ob diabetes model. Furthermore, we demonstrate rescue of Nephrin expression and cell viability in ebf2−/− primary podocytes cultured in high glucose. |
url |
http://www.sciencedirect.com/science/article/pii/S2211124715006828 |
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