Hayes Yard virus: a novel ephemerovirus isolated from a bull with severe clinical signs of bovine ephemeral fever is most closely related to Puchong virus

Abstract Bovine ephemeral fever is a vector-borne disease of ruminants that occurs in tropical and sub-tropical regions of Africa, Asia and Australia. The disease is caused by a rhabdovirus, bovine ephemeral fever virus (BEFV), which occurs as a single serotype globally. Although several other close...

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Main Authors: Kim R. Blasdell, Steven S. Davis, Rhonda Voysey, Dieter M. Bulach, Deborah Middleton, Sinead Williams, Margaret B. Harmsen, Richard P. Weir, Sandra Crameri, Susan J. Walsh, Grantley R. Peck, Robert B. Tesh, David B. Boyle, Lorna F. Melville, Peter J. Walker
Format: Article
Language:English
Published: BMC 2020-04-01
Series:Veterinary Research
Online Access:http://link.springer.com/article/10.1186/s13567-020-00781-1
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spelling doaj-68c7970cc76344d59200148a708b72f02020-11-25T03:04:37ZengBMCVeterinary Research1297-97162020-04-0151111310.1186/s13567-020-00781-1Hayes Yard virus: a novel ephemerovirus isolated from a bull with severe clinical signs of bovine ephemeral fever is most closely related to Puchong virusKim R. Blasdell0Steven S. Davis1Rhonda Voysey2Dieter M. Bulach3Deborah Middleton4Sinead Williams5Margaret B. Harmsen6Richard P. Weir7Sandra Crameri8Susan J. Walsh9Grantley R. Peck10Robert B. Tesh11David B. Boyle12Lorna F. Melville13Peter J. Walker14CSIRO Health and Biosecurity, Australian Animal Health LaboratoryBerrimah Veterinary Laboratories, Department of Primary Industry and FisheriesCSIRO Health and Biosecurity, Australian Animal Health LaboratoryMelbourne Bioinformatics, The University of MelbourneCSIRO Health and Biosecurity, Australian Animal Health LaboratoryCSIRO Health and Biosecurity, Australian Animal Health LaboratoryBerrimah Veterinary Laboratories, Department of Primary Industry and FisheriesBerrimah Veterinary Laboratories, Department of Primary Industry and FisheriesCSIRO Health and Biosecurity, Australian Animal Health LaboratoryBerrimah Veterinary Laboratories, Department of Primary Industry and FisheriesCSIRO Health and Biosecurity, Australian Animal Health LaboratoryCenter for Biodefense and Emerging Infectious Diseases, Department of Pathology, University of Texas Medical BranchCSIRO Health and Biosecurity, Australian Animal Health LaboratoryBerrimah Veterinary Laboratories, Department of Primary Industry and FisheriesCSIRO Health and Biosecurity, Australian Animal Health LaboratoryAbstract Bovine ephemeral fever is a vector-borne disease of ruminants that occurs in tropical and sub-tropical regions of Africa, Asia and Australia. The disease is caused by a rhabdovirus, bovine ephemeral fever virus (BEFV), which occurs as a single serotype globally. Although several other closely related ephemeroviruses have been isolated from cattle and/or arthropods, only kotonkan virus from Nigeria and (tentatively) Mavingoni virus from Mayotte Island in the Indian Ocean have been previously associated with febrile disease. Here, we report the isolation of a novel virus (Hayes Yard virus; HYV) from blood collected in February 2000 from a bull (Bos indicus) in the Northern Territory of Australia. The animal was suffering from a severe ephemeral fever-like illness with neurological involvement, including recumbency and paralysis, and was euthanised. Histological examination of spinal cord and lung tissue identified extensive haemorrhage in the dura mata with moderate perineuronal oedema and extensive emphysema. HYV displayed cone-shaped morphology, typical of rhabdoviruses, and was found to be most closely related antigenically to Puchong virus (PUCV), isolated in 1965 from mosquitoes in Malaysia. Analysis of complete genome sequences of HYV (15 025 nt) and PUCV (14 932 nt) indicated that each has a complex organisation (3′ N-P-M-G-GNS-α1-α2-β-γ-L 5′) and expression strategy, similar to that of BEFV. Based on an alignment of complete L protein sequences, HYV and PUCV cluster with other rhabdoviruses in the genus Ephemerovirus and appear to represent two new species. Neutralising antibody to HYV was also detected in a retrospective survey of cattle sera collected in the Northern Territory.http://link.springer.com/article/10.1186/s13567-020-00781-1
collection DOAJ
language English
format Article
sources DOAJ
author Kim R. Blasdell
Steven S. Davis
Rhonda Voysey
Dieter M. Bulach
Deborah Middleton
Sinead Williams
Margaret B. Harmsen
Richard P. Weir
Sandra Crameri
Susan J. Walsh
Grantley R. Peck
Robert B. Tesh
David B. Boyle
Lorna F. Melville
Peter J. Walker
spellingShingle Kim R. Blasdell
Steven S. Davis
Rhonda Voysey
Dieter M. Bulach
Deborah Middleton
Sinead Williams
Margaret B. Harmsen
Richard P. Weir
Sandra Crameri
Susan J. Walsh
Grantley R. Peck
Robert B. Tesh
David B. Boyle
Lorna F. Melville
Peter J. Walker
Hayes Yard virus: a novel ephemerovirus isolated from a bull with severe clinical signs of bovine ephemeral fever is most closely related to Puchong virus
Veterinary Research
author_facet Kim R. Blasdell
Steven S. Davis
Rhonda Voysey
Dieter M. Bulach
Deborah Middleton
Sinead Williams
Margaret B. Harmsen
Richard P. Weir
Sandra Crameri
Susan J. Walsh
Grantley R. Peck
Robert B. Tesh
David B. Boyle
Lorna F. Melville
Peter J. Walker
author_sort Kim R. Blasdell
title Hayes Yard virus: a novel ephemerovirus isolated from a bull with severe clinical signs of bovine ephemeral fever is most closely related to Puchong virus
title_short Hayes Yard virus: a novel ephemerovirus isolated from a bull with severe clinical signs of bovine ephemeral fever is most closely related to Puchong virus
title_full Hayes Yard virus: a novel ephemerovirus isolated from a bull with severe clinical signs of bovine ephemeral fever is most closely related to Puchong virus
title_fullStr Hayes Yard virus: a novel ephemerovirus isolated from a bull with severe clinical signs of bovine ephemeral fever is most closely related to Puchong virus
title_full_unstemmed Hayes Yard virus: a novel ephemerovirus isolated from a bull with severe clinical signs of bovine ephemeral fever is most closely related to Puchong virus
title_sort hayes yard virus: a novel ephemerovirus isolated from a bull with severe clinical signs of bovine ephemeral fever is most closely related to puchong virus
publisher BMC
series Veterinary Research
issn 1297-9716
publishDate 2020-04-01
description Abstract Bovine ephemeral fever is a vector-borne disease of ruminants that occurs in tropical and sub-tropical regions of Africa, Asia and Australia. The disease is caused by a rhabdovirus, bovine ephemeral fever virus (BEFV), which occurs as a single serotype globally. Although several other closely related ephemeroviruses have been isolated from cattle and/or arthropods, only kotonkan virus from Nigeria and (tentatively) Mavingoni virus from Mayotte Island in the Indian Ocean have been previously associated with febrile disease. Here, we report the isolation of a novel virus (Hayes Yard virus; HYV) from blood collected in February 2000 from a bull (Bos indicus) in the Northern Territory of Australia. The animal was suffering from a severe ephemeral fever-like illness with neurological involvement, including recumbency and paralysis, and was euthanised. Histological examination of spinal cord and lung tissue identified extensive haemorrhage in the dura mata with moderate perineuronal oedema and extensive emphysema. HYV displayed cone-shaped morphology, typical of rhabdoviruses, and was found to be most closely related antigenically to Puchong virus (PUCV), isolated in 1965 from mosquitoes in Malaysia. Analysis of complete genome sequences of HYV (15 025 nt) and PUCV (14 932 nt) indicated that each has a complex organisation (3′ N-P-M-G-GNS-α1-α2-β-γ-L 5′) and expression strategy, similar to that of BEFV. Based on an alignment of complete L protein sequences, HYV and PUCV cluster with other rhabdoviruses in the genus Ephemerovirus and appear to represent two new species. Neutralising antibody to HYV was also detected in a retrospective survey of cattle sera collected in the Northern Territory.
url http://link.springer.com/article/10.1186/s13567-020-00781-1
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