BIOCOMPATIBILITY OF IN HOUSE β-TRICALCIUM PHOSPHATE CERAMICS WITH NORMAL HUMAN OSTEOBLAST CELL
β-Tricalcium Phosphate (β–TCP) have been widely used as an implant materials. It has been successfully produced locally using two different method which is hydrothermal and precipitation. The aim of the study was to determine the biocompatibility of β-TCP prepared by hydrothermal and precipitation...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor's University
2012-04-01
|
Series: | Journal of Engineering Science and Technology |
Subjects: | |
Online Access: | http://jestec.taylors.edu.my/Vol%207%20Issue%202%20April%2012/Vol_7_2_169_176_DASMAWATI%20MOHAMAD.pdf |
id |
doaj-68c7b0caeae4402db689a0ba65dacef4 |
---|---|
record_format |
Article |
spelling |
doaj-68c7b0caeae4402db689a0ba65dacef42020-11-24T22:51:36ZengTaylor's UniversityJournal of Engineering Science and Technology1823-46902012-04-0172169176BIOCOMPATIBILITY OF IN HOUSE β-TRICALCIUM PHOSPHATE CERAMICS WITH NORMAL HUMAN OSTEOBLAST CELLNURSHUHADA MOHD NAZIRDASMAWATI MOHAMADMD. AZMAN SEENI MOHAMEDNOR SHAMSURIA OMARRADZALI OTHMANβ-Tricalcium Phosphate (β–TCP) have been widely used as an implant materials. It has been successfully produced locally using two different method which is hydrothermal and precipitation. The aim of the study was to determine the biocompatibility of β-TCP prepared by hydrothermal and precipitation method with normal human osteoblast (NHOst) cells. For this purpose cytotoxicity of the material was assessed using an Alamar Blue method to determine the viability of NHOst cells grown with extracts of β-TCP in various concentrations. In addition NHOst grown on β-TCP ceramics were examined under an inverted microscope after 4, 24, 48 and 72 hours to verify cell attachment. Staining was done using Calcein AM and Ethidium homodimers to assess viability using a Confocal Laser Scanning Microscope (CLSM). The results showed that neither hydrothermal β-TCP nor precipitation β-TCP were cytotoxic with either of the method applied.http://jestec.taylors.edu.my/Vol%207%20Issue%202%20April%2012/Vol_7_2_169_176_DASMAWATI%20MOHAMAD.pdfβ-Tricalcium phosphateCytotoxicityAttachmentCLSMOsteoblast |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
NURSHUHADA MOHD NAZIR DASMAWATI MOHAMAD MD. AZMAN SEENI MOHAMED NOR SHAMSURIA OMAR RADZALI OTHMAN |
spellingShingle |
NURSHUHADA MOHD NAZIR DASMAWATI MOHAMAD MD. AZMAN SEENI MOHAMED NOR SHAMSURIA OMAR RADZALI OTHMAN BIOCOMPATIBILITY OF IN HOUSE β-TRICALCIUM PHOSPHATE CERAMICS WITH NORMAL HUMAN OSTEOBLAST CELL Journal of Engineering Science and Technology β-Tricalcium phosphate Cytotoxicity Attachment CLSM Osteoblast |
author_facet |
NURSHUHADA MOHD NAZIR DASMAWATI MOHAMAD MD. AZMAN SEENI MOHAMED NOR SHAMSURIA OMAR RADZALI OTHMAN |
author_sort |
NURSHUHADA MOHD NAZIR |
title |
BIOCOMPATIBILITY OF IN HOUSE β-TRICALCIUM PHOSPHATE CERAMICS WITH NORMAL HUMAN OSTEOBLAST CELL |
title_short |
BIOCOMPATIBILITY OF IN HOUSE β-TRICALCIUM PHOSPHATE CERAMICS WITH NORMAL HUMAN OSTEOBLAST CELL |
title_full |
BIOCOMPATIBILITY OF IN HOUSE β-TRICALCIUM PHOSPHATE CERAMICS WITH NORMAL HUMAN OSTEOBLAST CELL |
title_fullStr |
BIOCOMPATIBILITY OF IN HOUSE β-TRICALCIUM PHOSPHATE CERAMICS WITH NORMAL HUMAN OSTEOBLAST CELL |
title_full_unstemmed |
BIOCOMPATIBILITY OF IN HOUSE β-TRICALCIUM PHOSPHATE CERAMICS WITH NORMAL HUMAN OSTEOBLAST CELL |
title_sort |
biocompatibility of in house β-tricalcium phosphate ceramics with normal human osteoblast cell |
publisher |
Taylor's University |
series |
Journal of Engineering Science and Technology |
issn |
1823-4690 |
publishDate |
2012-04-01 |
description |
β-Tricalcium Phosphate (β–TCP) have been widely used as an implant materials. It has been successfully produced locally using two different method which is hydrothermal and precipitation. The aim of the study was to determine the biocompatibility of β-TCP prepared by hydrothermal and precipitation method with normal human osteoblast (NHOst) cells. For this purpose cytotoxicity of the material was assessed using an Alamar Blue method to determine the viability of NHOst cells grown with extracts of β-TCP in various concentrations. In addition NHOst grown on β-TCP ceramics were examined under an inverted microscope after 4, 24, 48 and 72 hours to verify cell attachment. Staining was done using Calcein AM and Ethidium homodimers to assess viability using a Confocal Laser Scanning Microscope (CLSM). The results showed that neither hydrothermal β-TCP nor precipitation β-TCP were cytotoxic with either of the method applied. |
topic |
β-Tricalcium phosphate Cytotoxicity Attachment CLSM Osteoblast |
url |
http://jestec.taylors.edu.my/Vol%207%20Issue%202%20April%2012/Vol_7_2_169_176_DASMAWATI%20MOHAMAD.pdf |
work_keys_str_mv |
AT nurshuhadamohdnazir biocompatibilityofinhousebtricalciumphosphateceramicswithnormalhumanosteoblastcell AT dasmawatimohamad biocompatibilityofinhousebtricalciumphosphateceramicswithnormalhumanosteoblastcell AT mdazmanseenimohamed biocompatibilityofinhousebtricalciumphosphateceramicswithnormalhumanosteoblastcell AT norshamsuriaomar biocompatibilityofinhousebtricalciumphosphateceramicswithnormalhumanosteoblastcell AT radzaliothman biocompatibilityofinhousebtricalciumphosphateceramicswithnormalhumanosteoblastcell |
_version_ |
1725668846870724608 |