Comparative pharmacokinetic analysis of raw and steamed Panax notoginseng roots in rats by UPLC-MS/MS for simultaneously quantifying seven saponins
Context After being steamed, the restorative effects of Panax notoginseng (Burk.) F. H. Chen (Araliaceae) will be strengthened. However, the underlying mechanism remains elusive. Objective To compare the pharmacokinetics of ginsenosides Rg1, Rb1, Rd, Re, Rg5, Rk1, notoginsenoside R1 (GRg1, GRb1, GRd...
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doaj-68d16e1e9a324982abbbd3da5270c5872021-06-11T09:33:06ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162021-01-0159165366110.1080/13880209.2021.19282391928239Comparative pharmacokinetic analysis of raw and steamed Panax notoginseng roots in rats by UPLC-MS/MS for simultaneously quantifying seven saponinsJiajia Dong0Zhenzhen Yin1Lianlin Su2Mengting Yu3Meng Wang4Lin Li5Chunqin Mao6Tulin Lu7College of Pharmacy, Nanjing University of Chinese MedicineCollege of Pharmacy, Nanjing University of Chinese MedicineCollege of Pharmacy, Nanjing University of Chinese MedicineCollege of Pharmacy, Nanjing University of Chinese MedicineCollege of Pharmacy, Nanjing University of Chinese MedicineCollege of Pharmacy, Nanjing University of Chinese MedicineCollege of Pharmacy, Nanjing University of Chinese MedicineCollege of Pharmacy, Nanjing University of Chinese MedicineContext After being steamed, the restorative effects of Panax notoginseng (Burk.) F. H. Chen (Araliaceae) will be strengthened. However, the underlying mechanism remains elusive. Objective To compare the pharmacokinetics of ginsenosides Rg1, Rb1, Rd, Re, Rg5, Rk1, notoginsenoside R1 (GRg1, GRb1, GRd, GRe, GRg5, GRk1 and NGR1) in the raw and steam-processed P. notoginseng (RPN and SPN). Materials and methods The pharmacokinetics of seven components after oral administration of SPN and RPN extracts (1.0 g/kg) were investigated, respectively, in SD rats (two groups, n = 6) using UPLC-MS/MS. Results The approach elicited good linear regression (r2 > 0.991). The accuracy, precision and stability were all within ± 15%. The extraction recoveries and matrix effects were 75.0–100.8% and 85.1–110.3%, respectively. Compared with the RPN group, AUC0–t of GRg1 (176.63 ± 42.49 ng/h/mL), GRb1 (5094.06 ± 1453.14 ng/h/mL), GRd (1396.89 ± 595.14 ng/h/mL), and NGR1 (135.95 ± 54.32 ng/h/mL), along with Cmax of GRg1 (17.41 ± 5.43 ng/mL), GRb1 (361.48 ± 165.57 ng/mL), GRd (62.47 ± 33.65 ng/mL) and NGR1 (23.97 ± 16.77 ng/mL) decreased remarkably with oral administration of the SPN extracts, while GRe showed no significantly difference. Of note, GRg5 and GRk1 could not be detected in the plasma. Conclusions Influence of the processing reduced the systemic exposure levels to GRg1, GRb1, GRd and NGR1. It is the first report of comparative pharmacokinetic study of multiple saponins analysis after oral administration of RPN and SPN extract, which might be helpful for further studies on its steam-processing mechanism.http://dx.doi.org/10.1080/13880209.2021.1928239san-qiginsenosidessteam-processedtraditional chinese medicine |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jiajia Dong Zhenzhen Yin Lianlin Su Mengting Yu Meng Wang Lin Li Chunqin Mao Tulin Lu |
spellingShingle |
Jiajia Dong Zhenzhen Yin Lianlin Su Mengting Yu Meng Wang Lin Li Chunqin Mao Tulin Lu Comparative pharmacokinetic analysis of raw and steamed Panax notoginseng roots in rats by UPLC-MS/MS for simultaneously quantifying seven saponins Pharmaceutical Biology san-qi ginsenosides steam-processed traditional chinese medicine |
author_facet |
Jiajia Dong Zhenzhen Yin Lianlin Su Mengting Yu Meng Wang Lin Li Chunqin Mao Tulin Lu |
author_sort |
Jiajia Dong |
title |
Comparative pharmacokinetic analysis of raw and steamed Panax notoginseng roots in rats by UPLC-MS/MS for simultaneously quantifying seven saponins |
title_short |
Comparative pharmacokinetic analysis of raw and steamed Panax notoginseng roots in rats by UPLC-MS/MS for simultaneously quantifying seven saponins |
title_full |
Comparative pharmacokinetic analysis of raw and steamed Panax notoginseng roots in rats by UPLC-MS/MS for simultaneously quantifying seven saponins |
title_fullStr |
Comparative pharmacokinetic analysis of raw and steamed Panax notoginseng roots in rats by UPLC-MS/MS for simultaneously quantifying seven saponins |
title_full_unstemmed |
Comparative pharmacokinetic analysis of raw and steamed Panax notoginseng roots in rats by UPLC-MS/MS for simultaneously quantifying seven saponins |
title_sort |
comparative pharmacokinetic analysis of raw and steamed panax notoginseng roots in rats by uplc-ms/ms for simultaneously quantifying seven saponins |
publisher |
Taylor & Francis Group |
series |
Pharmaceutical Biology |
issn |
1388-0209 1744-5116 |
publishDate |
2021-01-01 |
description |
Context After being steamed, the restorative effects of Panax notoginseng (Burk.) F. H. Chen (Araliaceae) will be strengthened. However, the underlying mechanism remains elusive. Objective To compare the pharmacokinetics of ginsenosides Rg1, Rb1, Rd, Re, Rg5, Rk1, notoginsenoside R1 (GRg1, GRb1, GRd, GRe, GRg5, GRk1 and NGR1) in the raw and steam-processed P. notoginseng (RPN and SPN). Materials and methods The pharmacokinetics of seven components after oral administration of SPN and RPN extracts (1.0 g/kg) were investigated, respectively, in SD rats (two groups, n = 6) using UPLC-MS/MS. Results The approach elicited good linear regression (r2 > 0.991). The accuracy, precision and stability were all within ± 15%. The extraction recoveries and matrix effects were 75.0–100.8% and 85.1–110.3%, respectively. Compared with the RPN group, AUC0–t of GRg1 (176.63 ± 42.49 ng/h/mL), GRb1 (5094.06 ± 1453.14 ng/h/mL), GRd (1396.89 ± 595.14 ng/h/mL), and NGR1 (135.95 ± 54.32 ng/h/mL), along with Cmax of GRg1 (17.41 ± 5.43 ng/mL), GRb1 (361.48 ± 165.57 ng/mL), GRd (62.47 ± 33.65 ng/mL) and NGR1 (23.97 ± 16.77 ng/mL) decreased remarkably with oral administration of the SPN extracts, while GRe showed no significantly difference. Of note, GRg5 and GRk1 could not be detected in the plasma. Conclusions Influence of the processing reduced the systemic exposure levels to GRg1, GRb1, GRd and NGR1. It is the first report of comparative pharmacokinetic study of multiple saponins analysis after oral administration of RPN and SPN extract, which might be helpful for further studies on its steam-processing mechanism. |
topic |
san-qi ginsenosides steam-processed traditional chinese medicine |
url |
http://dx.doi.org/10.1080/13880209.2021.1928239 |
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