Cultural Antigen in the Technology for Anti-Rabies Immunoglobulin Obtainment from Equine Blood Serum

• Main Authors: S. V. Generalov, E. G. Abramova, Zh. V. Matveeva, I. M. Zhulidov, O. A. Lobovikova, R. A. Svintsov, A. V. Komissarov, M. N. Kireev, A. K. Nikiforov
• Format: Article
• Language: Russian
• Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2012-08-01
• Series: Проблемы особо опасных инфекций
• Subjects: антирабический иммуноглобулин; вирус бешенства; культуральный антиген; anti-rabies immunoglobulin; rabies virus; cultural antigen
• Online Access: https://journal.microbe.ru/jour/article/view/876
• ISSN: 0370-1069; 2658-719X

week after immunization (specific activity is identified using neutralization reaction on the model of white mice and dot-blot immunoassay). This level of activity is sufficient for the fractioning of immune serum and extraction of anti-rabies immunoglobulin. Physicochemical and biological properties of the anti-rabies immunoglobulin, obtained with the help of cultural antigen technique, meet the requirements stated in the normative documentation on anti-rabies immunoglobulins extracted from equine blood serum. Specific activity level of experimental batches of anti-rabies immunoglobulin, obtained with the help of cultural technologies, corresponds to 242 and 214 IU/ml.