Enhancing astaxanthin accumulation in Xanthophyllomyces dendrorhous by a phytohormone: metabolomic and gene expression profiles
Summary Xanthophyllomyces dendrorhous is a promising source of natural astaxanthin due to its ability to accumulate high amounts of astaxanthin. This study showed that 6‐benzylaminopurine (6‐BAP) is an effective substrate that enhances cell biomass and astaxanthin accumulation in X. dendrorhous. In...
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doaj-68e6e4a8b0c44c81b9a627fa3177f89c2020-11-25T03:51:25ZengWileyMicrobial Biotechnology1751-79152020-09-011351446146010.1111/1751-7915.13567Enhancing astaxanthin accumulation in Xanthophyllomyces dendrorhous by a phytohormone: metabolomic and gene expression profilesXueshan Pan0Baobei Wang1Ran Duan2Jing Jia3Jun Li4Weide Xiong5Xueping Ling6Cuixue Chen7Xiaohong Huang8Guoliang Zhang9Yinghua Lu10Department of Chemical and Biochemical Engineering College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 ChinaCollege of Oceanology and Food Science Quanzhou Normal University Quanzhou 362000 ChinaDepartment of Chemical and Biochemical Engineering College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 ChinaSDIC Biotechnology Investment Co. Ltd State Development and Investment Corporation Beijing 100034 ChinaDepartment of Chemical and Biochemical Engineering College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 ChinaDepartment of Chemical and Biochemical Engineering College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 ChinaDepartment of Chemical and Biochemical Engineering College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 ChinaDepartment of Chemical and Biochemical Engineering College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 ChinaDepartment of Stomatology Medical College Xiamen University Xiamen 361005 ChinaInstitute of Oceanic and Environmental Chemical Engineering State Key Lab Breeding Base of Green Chemical Synthesis Technology Zhejiang University of Technology Hangzhou 310014 ChinaDepartment of Chemical and Biochemical Engineering College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 ChinaSummary Xanthophyllomyces dendrorhous is a promising source of natural astaxanthin due to its ability to accumulate high amounts of astaxanthin. This study showed that 6‐benzylaminopurine (6‐BAP) is an effective substrate that enhances cell biomass and astaxanthin accumulation in X. dendrorhous. In the current study, the biomass and astaxanthin content in X. dendrorhous were determined to be improved by 21.98% and 24.20%, respectively, induced by 6‐BAP treatments. To further understand the metabolic responses of X. dendrorhous to 6‐BAP, time‐course metabolomics and gene expression levels of X. dendrorhous cultures with and without 6‐BAP feeding were investigated. Metabolome analysis revealed that 6‐BAP facilitated glucose consumption, promoted the glycolysis, suppressed the TCA cycle, drove carbon flux of acetyl‐CoA into fatty acid and mevalonate biosynthesis, and finally facilitated the formation of astaxanthin. ROS analysis suggested that the antioxidant mechanism in X. dendrorhous can be induced by 6‐BAP. Additionally, the process of 6‐BAP significantly upregulated the expression of six key genes involved in pathways related to astaxanthin biosynthesis. This research demonstrates the metabolomic mechanism of phytohormone stimulation of astaxanthin production iNn X. dendrorhous and presents a new strategy to improve astaxanthin production to prevent the dilemma of choosing between accumulation of astaxanthin and cell biomass.https://doi.org/10.1111/1751-7915.13567 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xueshan Pan Baobei Wang Ran Duan Jing Jia Jun Li Weide Xiong Xueping Ling Cuixue Chen Xiaohong Huang Guoliang Zhang Yinghua Lu |
spellingShingle |
Xueshan Pan Baobei Wang Ran Duan Jing Jia Jun Li Weide Xiong Xueping Ling Cuixue Chen Xiaohong Huang Guoliang Zhang Yinghua Lu Enhancing astaxanthin accumulation in Xanthophyllomyces dendrorhous by a phytohormone: metabolomic and gene expression profiles Microbial Biotechnology |
author_facet |
Xueshan Pan Baobei Wang Ran Duan Jing Jia Jun Li Weide Xiong Xueping Ling Cuixue Chen Xiaohong Huang Guoliang Zhang Yinghua Lu |
author_sort |
Xueshan Pan |
title |
Enhancing astaxanthin accumulation in Xanthophyllomyces dendrorhous by a phytohormone: metabolomic and gene expression profiles |
title_short |
Enhancing astaxanthin accumulation in Xanthophyllomyces dendrorhous by a phytohormone: metabolomic and gene expression profiles |
title_full |
Enhancing astaxanthin accumulation in Xanthophyllomyces dendrorhous by a phytohormone: metabolomic and gene expression profiles |
title_fullStr |
Enhancing astaxanthin accumulation in Xanthophyllomyces dendrorhous by a phytohormone: metabolomic and gene expression profiles |
title_full_unstemmed |
Enhancing astaxanthin accumulation in Xanthophyllomyces dendrorhous by a phytohormone: metabolomic and gene expression profiles |
title_sort |
enhancing astaxanthin accumulation in xanthophyllomyces dendrorhous by a phytohormone: metabolomic and gene expression profiles |
publisher |
Wiley |
series |
Microbial Biotechnology |
issn |
1751-7915 |
publishDate |
2020-09-01 |
description |
Summary Xanthophyllomyces dendrorhous is a promising source of natural astaxanthin due to its ability to accumulate high amounts of astaxanthin. This study showed that 6‐benzylaminopurine (6‐BAP) is an effective substrate that enhances cell biomass and astaxanthin accumulation in X. dendrorhous. In the current study, the biomass and astaxanthin content in X. dendrorhous were determined to be improved by 21.98% and 24.20%, respectively, induced by 6‐BAP treatments. To further understand the metabolic responses of X. dendrorhous to 6‐BAP, time‐course metabolomics and gene expression levels of X. dendrorhous cultures with and without 6‐BAP feeding were investigated. Metabolome analysis revealed that 6‐BAP facilitated glucose consumption, promoted the glycolysis, suppressed the TCA cycle, drove carbon flux of acetyl‐CoA into fatty acid and mevalonate biosynthesis, and finally facilitated the formation of astaxanthin. ROS analysis suggested that the antioxidant mechanism in X. dendrorhous can be induced by 6‐BAP. Additionally, the process of 6‐BAP significantly upregulated the expression of six key genes involved in pathways related to astaxanthin biosynthesis. This research demonstrates the metabolomic mechanism of phytohormone stimulation of astaxanthin production iNn X. dendrorhous and presents a new strategy to improve astaxanthin production to prevent the dilemma of choosing between accumulation of astaxanthin and cell biomass. |
url |
https://doi.org/10.1111/1751-7915.13567 |
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