Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20A
We here identified human leukocyte antigen-(HLA-)A∗2402-restricted epitope peptides from Cadherin 3, type 1, P-cadherin (CDH3) and kinesin family member 20A (KIF20A) that were found to be specifically expressed in cancer cells through genome-wide expression profile analysis. CDH3-10-807 peptide and...
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doaj-68f0ef22997e4fd280df0882b2828e742020-11-24T21:28:51ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512012-01-01201210.1155/2012/848042848042Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20ARyuji Osawa0Takuya Tsunoda1Sachiko Yoshimura2Tomohisa Watanabe3Motoki Miyazawa4Masaji Tani5Kazuyoshi Takeda6Hidewaki Nakagawa7Yusuke Nakamura8Hiroki Yamaue9Second Department of Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8510, JapanSecond Department of Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8510, JapanSecond Department of Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8510, JapanOncoTherapy Science Inc. Research Department, Kanagawa 213-0012, JapanSecond Department of Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8510, JapanSecond Department of Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8510, JapanDepartment of Immunology, Juntendo University School of Medicine, Tokyo 113-8421, JapanLaboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, JapanLaboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, JapanSecond Department of Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama 641-8510, JapanWe here identified human leukocyte antigen-(HLA-)A∗2402-restricted epitope peptides from Cadherin 3, type 1, P-cadherin (CDH3) and kinesin family member 20A (KIF20A) that were found to be specifically expressed in cancer cells through genome-wide expression profile analysis. CDH3-10-807 peptide and KIF20A-10-66 peptide successfully induced specific CTL clones, and these selectively responded to COS7 cells expressing both HLA-A∗2402 and respective protein while did not respond to parental cells or COS7 cells expressing either HLA-A∗2402 or respective protein. Furthermore, CTL clones responded to cancer cells that endogenously express HLA-A∗2402 and respective protein, suggesting that CDH3-10-807 peptide and KIF20A-10-66 peptide are naturally presented on HLA-A∗2402 molecule of human cancer cells. Our results demonstrated that CDH3-10-807 peptide and KIF20A-10-66 peptide are novel HLA-A24-restricted tumor-associated antigens and would be applicable for CTL-inducing cancer therapies.http://dx.doi.org/10.1155/2012/848042 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ryuji Osawa Takuya Tsunoda Sachiko Yoshimura Tomohisa Watanabe Motoki Miyazawa Masaji Tani Kazuyoshi Takeda Hidewaki Nakagawa Yusuke Nakamura Hiroki Yamaue |
spellingShingle |
Ryuji Osawa Takuya Tsunoda Sachiko Yoshimura Tomohisa Watanabe Motoki Miyazawa Masaji Tani Kazuyoshi Takeda Hidewaki Nakagawa Yusuke Nakamura Hiroki Yamaue Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20A Journal of Biomedicine and Biotechnology |
author_facet |
Ryuji Osawa Takuya Tsunoda Sachiko Yoshimura Tomohisa Watanabe Motoki Miyazawa Masaji Tani Kazuyoshi Takeda Hidewaki Nakagawa Yusuke Nakamura Hiroki Yamaue |
author_sort |
Ryuji Osawa |
title |
Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20A |
title_short |
Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20A |
title_full |
Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20A |
title_fullStr |
Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20A |
title_full_unstemmed |
Identification of HLA-A24-Restricted Novel T Cell Epitope Peptides Derived from P-Cadherin and Kinesin Family Member 20A |
title_sort |
identification of hla-a24-restricted novel t cell epitope peptides derived from p-cadherin and kinesin family member 20a |
publisher |
Hindawi Limited |
series |
Journal of Biomedicine and Biotechnology |
issn |
1110-7243 1110-7251 |
publishDate |
2012-01-01 |
description |
We here identified human leukocyte antigen-(HLA-)A∗2402-restricted epitope peptides from Cadherin 3, type 1, P-cadherin (CDH3) and kinesin family member 20A (KIF20A) that were found to be specifically expressed in cancer cells through genome-wide expression profile analysis. CDH3-10-807 peptide and KIF20A-10-66 peptide successfully induced specific CTL clones, and these selectively responded to COS7 cells expressing both HLA-A∗2402 and respective protein while did not respond to parental cells or COS7 cells expressing either HLA-A∗2402 or respective protein. Furthermore, CTL clones responded to cancer cells that endogenously express HLA-A∗2402 and respective protein, suggesting that CDH3-10-807 peptide and KIF20A-10-66 peptide are naturally presented on HLA-A∗2402 molecule of human cancer cells. Our results demonstrated that CDH3-10-807 peptide and KIF20A-10-66 peptide are novel HLA-A24-restricted tumor-associated antigens and would be applicable for CTL-inducing cancer therapies. |
url |
http://dx.doi.org/10.1155/2012/848042 |
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