Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides
Increased DNA damage and the propension to cancer development, depend on the modulation of the mechanisms to control and maintain genomic integrity. Poly(ADP-Ribose)Polymerase activation and automodification are early responses to genotoxic stress. Upon binding to DNA strand breaks, the enzyme, a mo...
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doaj-6924a59ce8f6495796a4e46ea692f6932020-11-24T21:21:34ZengMDPI AGCells2073-44092019-02-018213710.3390/cells8020137cells8020137Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to PesticidesSerena Imperato0Carmela Mistretta1Maria Marone2Ilaria Migliaccio3Ilaria Pulcinelli4Maria Rosaria Faraone Mennella5Department of Biology, University of Naples “Federico II”, Via Cintia, 80126 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, Via Cintia, 80126 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, Via Cintia, 80126 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, Via Cintia, 80126 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, Via Cintia, 80126 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, Via Cintia, 80126 Naples, ItalyIncreased DNA damage and the propension to cancer development, depend on the modulation of the mechanisms to control and maintain genomic integrity. Poly(ADP-Ribose)Polymerase activation and automodification are early responses to genotoxic stress. Upon binding to DNA strand breaks, the enzyme, a molecular DNA nick sensor, is hyperactivated: this is the first step in a series of events leading to either DNA repair or apoptosis. Enzyme hyperactivation and automodification can be easily measured and are widely used to look at DNA damage extent in the cell. We investigated whether these two markers (increased catalytic activity and auto modification), could help to monitor DNA damage in lymphocytes of flower growers from Southern Italy, occupationally exposed to pesticides. Peripheral lymphocyte lysates were analyzed for Poly(ADP-Ribose)Polymerase activity, and by SDS-PAGE and anti-Poly(ADP-Ribose)Polymerase 1-antibodyto measure automodified Poly(ADP-Ribose)Polymerase levels bydensitometry. Poly(ADP-Ribose)Polymerase activity and PARP automodification followed the same trend. Growers daily exposed to pesticides, showed both biomarkers very high, either in the presence or in the absence of pathologies. PARP activity and auto-modification in peripheral blood lymphocytes are possible, non-invasive, androutinartools to monitor the healthy conditions of floricoltorists.https://www.mdpi.com/2073-4409/8/2/137Poly(ADP-Ribose)Polymeraseautomodified PARP (PAR-PARP)DNA damagepesticidesgrowersgreenhouses |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Serena Imperato Carmela Mistretta Maria Marone Ilaria Migliaccio Ilaria Pulcinelli Maria Rosaria Faraone Mennella |
spellingShingle |
Serena Imperato Carmela Mistretta Maria Marone Ilaria Migliaccio Ilaria Pulcinelli Maria Rosaria Faraone Mennella Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides Cells Poly(ADP-Ribose)Polymerase automodified PARP (PAR-PARP) DNA damage pesticides growers greenhouses |
author_facet |
Serena Imperato Carmela Mistretta Maria Marone Ilaria Migliaccio Ilaria Pulcinelli Maria Rosaria Faraone Mennella |
author_sort |
Serena Imperato |
title |
Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides |
title_short |
Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides |
title_full |
Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides |
title_fullStr |
Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides |
title_full_unstemmed |
Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides |
title_sort |
automodified poly(adp-ribose) polymerase analysisto monitor dna damagein peripheral lymphocytes of floriculturists occupationally exposed to pesticides |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2019-02-01 |
description |
Increased DNA damage and the propension to cancer development, depend on the modulation of the mechanisms to control and maintain genomic integrity. Poly(ADP-Ribose)Polymerase activation and automodification are early responses to genotoxic stress. Upon binding to DNA strand breaks, the enzyme, a molecular DNA nick sensor, is hyperactivated: this is the first step in a series of events leading to either DNA repair or apoptosis. Enzyme hyperactivation and automodification can be easily measured and are widely used to look at DNA damage extent in the cell. We investigated whether these two markers (increased catalytic activity and auto modification), could help to monitor DNA damage in lymphocytes of flower growers from Southern Italy, occupationally exposed to pesticides. Peripheral lymphocyte lysates were analyzed for Poly(ADP-Ribose)Polymerase activity, and by SDS-PAGE and anti-Poly(ADP-Ribose)Polymerase 1-antibodyto measure automodified Poly(ADP-Ribose)Polymerase levels bydensitometry. Poly(ADP-Ribose)Polymerase activity and PARP automodification followed the same trend. Growers daily exposed to pesticides, showed both biomarkers very high, either in the presence or in the absence of pathologies. PARP activity and auto-modification in peripheral blood lymphocytes are possible, non-invasive, androutinartools to monitor the healthy conditions of floricoltorists. |
topic |
Poly(ADP-Ribose)Polymerase automodified PARP (PAR-PARP) DNA damage pesticides growers greenhouses |
url |
https://www.mdpi.com/2073-4409/8/2/137 |
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