Role of Erythromycin-Regulated Histone Deacetylase-2 in Benign Tracheal Stenosis
Objective. This study aims to explore the role of erythromycin-regulated histone deacetylase-2 in benign tracheal stenosis. Methods. The rabbit model of tracheal stenosis was established. The rabbits were randomly divided into 8 groups. Histone deacetylase-2 (HDAC2) expression was detected by immuno...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2020-01-01
|
Series: | Canadian Respiratory Journal |
Online Access: | http://dx.doi.org/10.1155/2020/4213807 |
id |
doaj-692ba94893c949fc9f228a8f6d4e5b00 |
---|---|
record_format |
Article |
spelling |
doaj-692ba94893c949fc9f228a8f6d4e5b002021-07-02T12:40:50ZengHindawi LimitedCanadian Respiratory Journal1198-22411916-72452020-01-01202010.1155/2020/42138074213807Role of Erythromycin-Regulated Histone Deacetylase-2 in Benign Tracheal StenosisZhenjie Huang0Peng Wei1Luoman Gan2Tonghua Zeng3Caicheng Qin4Guangnan Liu5Guangxi Medical University, Nanning, ChinaGuangxi Medical University, Nanning, ChinaMedical School of Qinghai University, Xining, ChinaDepartment of Respiratory Medicine, Beihai People’s Hospital, Beihai, ChinaGuangxi Medical University, Nanning, ChinaDepartment of Respiratory Medicine, The Second Affiliated Hospital of Guangxi Medical University, Nanning, ChinaObjective. This study aims to explore the role of erythromycin-regulated histone deacetylase-2 in benign tracheal stenosis. Methods. The rabbit model of tracheal stenosis was established. The rabbits were randomly divided into 8 groups. Histone deacetylase-2 (HDAC2) expression was detected by immunofluorescence. The expression of type I collagen and type III collagen was detected by immunohistochemical method. The expression of TGF-β1, VEGF and IL-8 in serum and alveolar lavage fluid was detected by ELISA. The expression of HDAC2, TGF-β1, VEGF and IL-8 in bronchi of each group was detected by Western blotting method. Results. In Erythromycin (ERY) group, ERY + Budesonide group, ERY + Vorinostat group and ERY + Budesonide + Vorinostat group, the degree of bronchial stenosis was alleviated, and the mucosal epithelium was still slightly proliferated. The effect of ERY combined with other drugs was more obvious. The HDAC2 protein expression increased significantly in ERY group, ERY + Budesonide group and ERY + Budesonide + Vorinostat group and decreased significantly in Vorinostat group, the expression of collagen I and III decreased significantly in ERY group, ERY + Budesonide group and ERY + Budesonide + Vorinostat group (P<0.05). The TGF-β1, IL-8 and VEGF levels decreased significantly in ERY group, ERY + Budesonide group, ERY + Vorinostat group and ERY + Budesonide + Vorinostat group (P<0.05). Conclusions. Erythromycin inhibited inflammation and excessive proliferation of granulation tissue after tracheobronchial mucosal injury by up-regulating the expression of HDAC2, it promoted wound healing and alleviated tracheobronchial stenosis. When combined with budesonide, penicillin and other glucocorticoids and antibiotics, it had a good synergistic effect. However, vorinostat could attenuate erythromycin’s effect by down-regulating the expression of HDAC2. It may have good clinical application prospects in the treatment of tracheal stenosis.http://dx.doi.org/10.1155/2020/4213807 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhenjie Huang Peng Wei Luoman Gan Tonghua Zeng Caicheng Qin Guangnan Liu |
spellingShingle |
Zhenjie Huang Peng Wei Luoman Gan Tonghua Zeng Caicheng Qin Guangnan Liu Role of Erythromycin-Regulated Histone Deacetylase-2 in Benign Tracheal Stenosis Canadian Respiratory Journal |
author_facet |
Zhenjie Huang Peng Wei Luoman Gan Tonghua Zeng Caicheng Qin Guangnan Liu |
author_sort |
Zhenjie Huang |
title |
Role of Erythromycin-Regulated Histone Deacetylase-2 in Benign Tracheal Stenosis |
title_short |
Role of Erythromycin-Regulated Histone Deacetylase-2 in Benign Tracheal Stenosis |
title_full |
Role of Erythromycin-Regulated Histone Deacetylase-2 in Benign Tracheal Stenosis |
title_fullStr |
Role of Erythromycin-Regulated Histone Deacetylase-2 in Benign Tracheal Stenosis |
title_full_unstemmed |
Role of Erythromycin-Regulated Histone Deacetylase-2 in Benign Tracheal Stenosis |
title_sort |
role of erythromycin-regulated histone deacetylase-2 in benign tracheal stenosis |
publisher |
Hindawi Limited |
series |
Canadian Respiratory Journal |
issn |
1198-2241 1916-7245 |
publishDate |
2020-01-01 |
description |
Objective. This study aims to explore the role of erythromycin-regulated histone deacetylase-2 in benign tracheal stenosis. Methods. The rabbit model of tracheal stenosis was established. The rabbits were randomly divided into 8 groups. Histone deacetylase-2 (HDAC2) expression was detected by immunofluorescence. The expression of type I collagen and type III collagen was detected by immunohistochemical method. The expression of TGF-β1, VEGF and IL-8 in serum and alveolar lavage fluid was detected by ELISA. The expression of HDAC2, TGF-β1, VEGF and IL-8 in bronchi of each group was detected by Western blotting method. Results. In Erythromycin (ERY) group, ERY + Budesonide group, ERY + Vorinostat group and ERY + Budesonide + Vorinostat group, the degree of bronchial stenosis was alleviated, and the mucosal epithelium was still slightly proliferated. The effect of ERY combined with other drugs was more obvious. The HDAC2 protein expression increased significantly in ERY group, ERY + Budesonide group and ERY + Budesonide + Vorinostat group and decreased significantly in Vorinostat group, the expression of collagen I and III decreased significantly in ERY group, ERY + Budesonide group and ERY + Budesonide + Vorinostat group (P<0.05). The TGF-β1, IL-8 and VEGF levels decreased significantly in ERY group, ERY + Budesonide group, ERY + Vorinostat group and ERY + Budesonide + Vorinostat group (P<0.05). Conclusions. Erythromycin inhibited inflammation and excessive proliferation of granulation tissue after tracheobronchial mucosal injury by up-regulating the expression of HDAC2, it promoted wound healing and alleviated tracheobronchial stenosis. When combined with budesonide, penicillin and other glucocorticoids and antibiotics, it had a good synergistic effect. However, vorinostat could attenuate erythromycin’s effect by down-regulating the expression of HDAC2. It may have good clinical application prospects in the treatment of tracheal stenosis. |
url |
http://dx.doi.org/10.1155/2020/4213807 |
work_keys_str_mv |
AT zhenjiehuang roleoferythromycinregulatedhistonedeacetylase2inbenigntrachealstenosis AT pengwei roleoferythromycinregulatedhistonedeacetylase2inbenigntrachealstenosis AT luomangan roleoferythromycinregulatedhistonedeacetylase2inbenigntrachealstenosis AT tonghuazeng roleoferythromycinregulatedhistonedeacetylase2inbenigntrachealstenosis AT caichengqin roleoferythromycinregulatedhistonedeacetylase2inbenigntrachealstenosis AT guangnanliu roleoferythromycinregulatedhistonedeacetylase2inbenigntrachealstenosis |
_version_ |
1721329791378915328 |