Screening of peptide libraries against protective antigen of Bacillus anthracis in a disposable microfluidic cartridge.
Bacterial surface peptide display has gained popularity as a method of affinity reagent generation for a wide variety of applications ranging from drug discovery to pathogen detection. In order to isolate the bacterial clones that express peptides with high affinities to the target molecule, multipl...
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2011-01-01
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doaj-694fa127694b47aaa600ab67047393032020-11-25T02:00:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01611e2692510.1371/journal.pone.0026925Screening of peptide libraries against protective antigen of Bacillus anthracis in a disposable microfluidic cartridge.Joshua M KogotYanting ZhangStephen J MoorePaul PaganoDimitra N Stratis-CullumDavid Chang-YenMarek TurewiczPaul M PellegrinoAndre de FuscoH Tom SohNancy E StaglianoBacterial surface peptide display has gained popularity as a method of affinity reagent generation for a wide variety of applications ranging from drug discovery to pathogen detection. In order to isolate the bacterial clones that express peptides with high affinities to the target molecule, multiple rounds of manual magnetic activated cell sorting (MACS) followed by multiple rounds of fluorescence activated cell sorting (FACS) are conventionally used. Although such manual methods are effective, alternative means of library screening which improve the reproducibility, reduce the cost, reduce cross contamination, and minimize exposure to hazardous target materials are highly desired for practical application. Toward this end, we report the first semi-automated system demonstrating the potential for screening bacterially displayed peptides using disposable microfluidic cartridges. The Micro-Magnetic Separation platform (MMS) is capable of screening a bacterial library containing 3 × 10¹⁰ members in 15 minutes and requires minimal operator training. Using this system, we report the isolation of twenty-four distinct peptide ligands that bind to the protective antigen (PA) of Bacilus anthracis in three rounds of selection. A consensus motif WXCFTC was found using the MMS and was also found in one of the PA binders isolated by the conventional MACS/FACS approach. We compared MMS and MACS rare cell recovery over cell populations ranging from 0.1% to 0.0000001% and found that both magnetic sorting methods could recover cells down to 0.0000001% initial cell population, with the MMS having overall lower standard deviation of cell recovery. We believe the MMS system offers a compelling approach towards highly efficient, semi-automated screening of molecular libraries that is at least equal to manual magnetic sorting methods and produced, for the first time, 15-mer peptide binders to PA protein that exhibit better affinity and specificity than peptides isolated using conventional MACS/FACS.http://europepmc.org/articles/PMC3225367?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Joshua M Kogot Yanting Zhang Stephen J Moore Paul Pagano Dimitra N Stratis-Cullum David Chang-Yen Marek Turewicz Paul M Pellegrino Andre de Fusco H Tom Soh Nancy E Stagliano |
spellingShingle |
Joshua M Kogot Yanting Zhang Stephen J Moore Paul Pagano Dimitra N Stratis-Cullum David Chang-Yen Marek Turewicz Paul M Pellegrino Andre de Fusco H Tom Soh Nancy E Stagliano Screening of peptide libraries against protective antigen of Bacillus anthracis in a disposable microfluidic cartridge. PLoS ONE |
author_facet |
Joshua M Kogot Yanting Zhang Stephen J Moore Paul Pagano Dimitra N Stratis-Cullum David Chang-Yen Marek Turewicz Paul M Pellegrino Andre de Fusco H Tom Soh Nancy E Stagliano |
author_sort |
Joshua M Kogot |
title |
Screening of peptide libraries against protective antigen of Bacillus anthracis in a disposable microfluidic cartridge. |
title_short |
Screening of peptide libraries against protective antigen of Bacillus anthracis in a disposable microfluidic cartridge. |
title_full |
Screening of peptide libraries against protective antigen of Bacillus anthracis in a disposable microfluidic cartridge. |
title_fullStr |
Screening of peptide libraries against protective antigen of Bacillus anthracis in a disposable microfluidic cartridge. |
title_full_unstemmed |
Screening of peptide libraries against protective antigen of Bacillus anthracis in a disposable microfluidic cartridge. |
title_sort |
screening of peptide libraries against protective antigen of bacillus anthracis in a disposable microfluidic cartridge. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
Bacterial surface peptide display has gained popularity as a method of affinity reagent generation for a wide variety of applications ranging from drug discovery to pathogen detection. In order to isolate the bacterial clones that express peptides with high affinities to the target molecule, multiple rounds of manual magnetic activated cell sorting (MACS) followed by multiple rounds of fluorescence activated cell sorting (FACS) are conventionally used. Although such manual methods are effective, alternative means of library screening which improve the reproducibility, reduce the cost, reduce cross contamination, and minimize exposure to hazardous target materials are highly desired for practical application. Toward this end, we report the first semi-automated system demonstrating the potential for screening bacterially displayed peptides using disposable microfluidic cartridges. The Micro-Magnetic Separation platform (MMS) is capable of screening a bacterial library containing 3 × 10¹⁰ members in 15 minutes and requires minimal operator training. Using this system, we report the isolation of twenty-four distinct peptide ligands that bind to the protective antigen (PA) of Bacilus anthracis in three rounds of selection. A consensus motif WXCFTC was found using the MMS and was also found in one of the PA binders isolated by the conventional MACS/FACS approach. We compared MMS and MACS rare cell recovery over cell populations ranging from 0.1% to 0.0000001% and found that both magnetic sorting methods could recover cells down to 0.0000001% initial cell population, with the MMS having overall lower standard deviation of cell recovery. We believe the MMS system offers a compelling approach towards highly efficient, semi-automated screening of molecular libraries that is at least equal to manual magnetic sorting methods and produced, for the first time, 15-mer peptide binders to PA protein that exhibit better affinity and specificity than peptides isolated using conventional MACS/FACS. |
url |
http://europepmc.org/articles/PMC3225367?pdf=render |
work_keys_str_mv |
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