Differential seroprevalence of human bocavirus species 1-4 in Beijing, China.
BACKGROUND: Four species of human bocaviruses (HBoV1-4) have been identified based on phylogenetic analysis since its first report in 2005. HBoV1 has been associated with respiratory disease, whereas HBoV2-4 are mainly detected in enteric infections. Although the prevalence of HBoVs in humans has be...
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doaj-6955230bb6624772b45bd348648886012020-11-24T23:48:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3964410.1371/journal.pone.0039644Differential seroprevalence of human bocavirus species 1-4 in Beijing, China.Li GuoYaying WangHongli ZhouChao WuJingdong SongJianguo LiGláucia Paranhos-BaccalàGuy VernetJianwei WangTao HungBACKGROUND: Four species of human bocaviruses (HBoV1-4) have been identified based on phylogenetic analysis since its first report in 2005. HBoV1 has been associated with respiratory disease, whereas HBoV2-4 are mainly detected in enteric infections. Although the prevalence of HBoVs in humans has been studied in some regions, it has not been well addressed globally. METHODOLOGY/PRINCIPAL FINDINGS: Cross-reactivity of anti-VP2 antibodies was detected between HBoV1, 2, 3, and 4 in mouse and human serum. The prevalence of specific anti-VP2 IgG antibodies against HBoV1-4 was determined in different age groups of healthy individuals aged 0-70 years old in Beijing, China, using a competition ELISA assay based on virus-like particles of HBoV1-4. The seroprevalence of HBoV1-4 was 50%, 36.9%, 28.7%, and 0.8%, respectively, in children aged 0-14 years (n = 244); whereas the seroprevalence of HBoV1-4 was 66.9%, 49.3%, 38.7% and 1.4%, respectively, in healthy adults (≥ 15 years old; n = 142). The seropositive rate of HBoV1 was higher than that of HBoV2, HBoV3, and HBoV4 in individuals older than 0.5 years. Furthermore, IgG seroconversion of HBoV1 (10/31, 32.3%), HBoV2 (8/31, 25.8%), and HBoV3 (2/31, 6.5%) was found in paired sera collected from children with respiratory tract infections who were positive for HBoV1 according to PCR analysis. CONCLUSIONS/SIGNIFICANCE: Our data indicate that HBoV1 is more prevalent than HBoV2, HBoV3, and HBoV4 in the population we sampled in Beijing, China, suggesting that HBoV species may play differential roles in disease.http://europepmc.org/articles/PMC3382199?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li Guo Yaying Wang Hongli Zhou Chao Wu Jingdong Song Jianguo Li Gláucia Paranhos-Baccalà Guy Vernet Jianwei Wang Tao Hung |
spellingShingle |
Li Guo Yaying Wang Hongli Zhou Chao Wu Jingdong Song Jianguo Li Gláucia Paranhos-Baccalà Guy Vernet Jianwei Wang Tao Hung Differential seroprevalence of human bocavirus species 1-4 in Beijing, China. PLoS ONE |
author_facet |
Li Guo Yaying Wang Hongli Zhou Chao Wu Jingdong Song Jianguo Li Gláucia Paranhos-Baccalà Guy Vernet Jianwei Wang Tao Hung |
author_sort |
Li Guo |
title |
Differential seroprevalence of human bocavirus species 1-4 in Beijing, China. |
title_short |
Differential seroprevalence of human bocavirus species 1-4 in Beijing, China. |
title_full |
Differential seroprevalence of human bocavirus species 1-4 in Beijing, China. |
title_fullStr |
Differential seroprevalence of human bocavirus species 1-4 in Beijing, China. |
title_full_unstemmed |
Differential seroprevalence of human bocavirus species 1-4 in Beijing, China. |
title_sort |
differential seroprevalence of human bocavirus species 1-4 in beijing, china. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
BACKGROUND: Four species of human bocaviruses (HBoV1-4) have been identified based on phylogenetic analysis since its first report in 2005. HBoV1 has been associated with respiratory disease, whereas HBoV2-4 are mainly detected in enteric infections. Although the prevalence of HBoVs in humans has been studied in some regions, it has not been well addressed globally. METHODOLOGY/PRINCIPAL FINDINGS: Cross-reactivity of anti-VP2 antibodies was detected between HBoV1, 2, 3, and 4 in mouse and human serum. The prevalence of specific anti-VP2 IgG antibodies against HBoV1-4 was determined in different age groups of healthy individuals aged 0-70 years old in Beijing, China, using a competition ELISA assay based on virus-like particles of HBoV1-4. The seroprevalence of HBoV1-4 was 50%, 36.9%, 28.7%, and 0.8%, respectively, in children aged 0-14 years (n = 244); whereas the seroprevalence of HBoV1-4 was 66.9%, 49.3%, 38.7% and 1.4%, respectively, in healthy adults (≥ 15 years old; n = 142). The seropositive rate of HBoV1 was higher than that of HBoV2, HBoV3, and HBoV4 in individuals older than 0.5 years. Furthermore, IgG seroconversion of HBoV1 (10/31, 32.3%), HBoV2 (8/31, 25.8%), and HBoV3 (2/31, 6.5%) was found in paired sera collected from children with respiratory tract infections who were positive for HBoV1 according to PCR analysis. CONCLUSIONS/SIGNIFICANCE: Our data indicate that HBoV1 is more prevalent than HBoV2, HBoV3, and HBoV4 in the population we sampled in Beijing, China, suggesting that HBoV species may play differential roles in disease. |
url |
http://europepmc.org/articles/PMC3382199?pdf=render |
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