Evaluation of [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz for Pretargeted Imaging in LS174T Xenografts—Comparison to [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-Tz
Pretargeted nuclear imaging for the diagnosis of various cancers is an emerging and fast developing field. The tetrazine ligation is currently considered the most promising reaction in this respect. Monoclonal antibodies are often the preferred choice as pretargeting vector due to their outstanding...
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doaj-695b146362c44a5b9aa2385af6372ae42021-01-22T00:04:42ZengMDPI AGMolecules1420-30492021-01-012654454410.3390/molecules26030544Evaluation of [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz for Pretargeted Imaging in LS174T Xenografts—Comparison to [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-TzChristian B. M. Poulie0Jesper T. Jørgensen1Vladimir Shalgunov2Georgios Kougioumtzoglou3Troels Elmer Jeppesen4Andreas Kjaer5Matthias M. Herth6Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 160, DK—2100 Copenhagen, DenmarkDepartment of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, DenmarkDepartment of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 160, DK—2100 Copenhagen, DenmarkDepartment of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 160, DK—2100 Copenhagen, DenmarkDepartment of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, DenmarkDepartment of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, DenmarkDepartment of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Jagtvej 160, DK—2100 Copenhagen, DenmarkPretargeted nuclear imaging for the diagnosis of various cancers is an emerging and fast developing field. The tetrazine ligation is currently considered the most promising reaction in this respect. Monoclonal antibodies are often the preferred choice as pretargeting vector due to their outstanding targeting properties. In this work, we evaluated the performance of [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz using a setup we previously used for [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-Tz, thereby allowing for comparison of the performance of these two promising pretargeting imaging agents. The evaluation included a comparison of the physicochemical properties of the compounds and their performance in an ex vivo blocking assay. Finally, [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz was evaluated in a pretargeted imaging study and compared to [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-Tz. Despite minor differences, this study indicated that both evaluated tetrazines are equally suited for pretargeted imaging.https://www.mdpi.com/1420-3049/26/3/544tetrazinepretargeted imagingradiochemistryPETSPECTcancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christian B. M. Poulie Jesper T. Jørgensen Vladimir Shalgunov Georgios Kougioumtzoglou Troels Elmer Jeppesen Andreas Kjaer Matthias M. Herth |
spellingShingle |
Christian B. M. Poulie Jesper T. Jørgensen Vladimir Shalgunov Georgios Kougioumtzoglou Troels Elmer Jeppesen Andreas Kjaer Matthias M. Herth Evaluation of [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz for Pretargeted Imaging in LS174T Xenografts—Comparison to [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-Tz Molecules tetrazine pretargeted imaging radiochemistry PET SPECT cancer |
author_facet |
Christian B. M. Poulie Jesper T. Jørgensen Vladimir Shalgunov Georgios Kougioumtzoglou Troels Elmer Jeppesen Andreas Kjaer Matthias M. Herth |
author_sort |
Christian B. M. Poulie |
title |
Evaluation of [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz for Pretargeted Imaging in LS174T Xenografts—Comparison to [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-Tz |
title_short |
Evaluation of [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz for Pretargeted Imaging in LS174T Xenografts—Comparison to [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-Tz |
title_full |
Evaluation of [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz for Pretargeted Imaging in LS174T Xenografts—Comparison to [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-Tz |
title_fullStr |
Evaluation of [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz for Pretargeted Imaging in LS174T Xenografts—Comparison to [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-Tz |
title_full_unstemmed |
Evaluation of [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz for Pretargeted Imaging in LS174T Xenografts—Comparison to [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-Tz |
title_sort |
evaluation of [<sup>64</sup>cu]cu-nota-peg<sub>7</sub>-h-tz for pretargeted imaging in ls174t xenografts—comparison to [<sup>111</sup>in]in-dota-peg<sub>11</sub>-bispy-tz |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-01-01 |
description |
Pretargeted nuclear imaging for the diagnosis of various cancers is an emerging and fast developing field. The tetrazine ligation is currently considered the most promising reaction in this respect. Monoclonal antibodies are often the preferred choice as pretargeting vector due to their outstanding targeting properties. In this work, we evaluated the performance of [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz using a setup we previously used for [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-Tz, thereby allowing for comparison of the performance of these two promising pretargeting imaging agents. The evaluation included a comparison of the physicochemical properties of the compounds and their performance in an ex vivo blocking assay. Finally, [<sup>64</sup>Cu]Cu-NOTA-PEG<sub>7</sub>-H-Tz was evaluated in a pretargeted imaging study and compared to [<sup>111</sup>In]In-DOTA-PEG<sub>11</sub>-BisPy-Tz. Despite minor differences, this study indicated that both evaluated tetrazines are equally suited for pretargeted imaging. |
topic |
tetrazine pretargeted imaging radiochemistry PET SPECT cancer |
url |
https://www.mdpi.com/1420-3049/26/3/544 |
work_keys_str_mv |
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