Rapid rare ABO blood typing using a single PCR based on a multiplex SNaPshot reaction
Background: ABO subgroups would be considered when discrepancies in ABO grouping occur. Serological methods including adsorption-elution test, salivary ABH inhibition test, and anti-A1 (lectin) saline method could be used. However, these serological methods are laboring and obscure. Therefore, relia...
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doaj-69619fb68f754677895bad7d267c78a32020-11-24T23:05:58ZengElsevierJournal of the Formosan Medical Association0929-66462019-01-011181395400Rapid rare ABO blood typing using a single PCR based on a multiplex SNaPshot reactionDing-Ping Chen0Ying-hao Wen1Jang-Jih Lu2Ching-Ping Tseng3Wei-Ting Wang4Department of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Corresponding author. Department of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City, 333, Taiwan.Department of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, TaiwanDepartment of Laboratory Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, TaiwanBackground: ABO subgroups would be considered when discrepancies in ABO grouping occur. Serological methods including adsorption-elution test, salivary ABH inhibition test, and anti-A1 (lectin) saline method could be used. However, these serological methods are laboring and obscure. Therefore, reliable and affordable method to assess the ABO subgroups is of particular interest. Methods: To solve this problem, the multiplex SNaPshot-based assays were designed to determine rare A and B subgroups. Primers used as probes for determination of rare ABO blood groups known in Taiwanese population were designed. Many ABO subtype samples were used to validate the accuracy and reproducibility of our SNaPshot panel. Results: A panel of primer probes were successfully designed in determining 8 SNP sites (261, 539, 838, 820, 745, 664, IVS6 +5, and 829 in exon 6 and 7) for A phenotype and 6 SNP sites (261, 796, IVS3 +5, 247, 523, and 502 in exon 2, 6 and 7 and intron 3) for B phenotype. SNaPshot analysis for defining blood group A alleles (A1, A2, A3, Am and Ael) and blood group B alleles (B1, B3, Bw and Bel) was therefore available. Conclusion: SNaPshot analysis could be used in reference laboratories for typing known rare subgroups of A and B without DNA cloning and traditional sequencing. Moreover, this method would help to construct databases of genotyped blood donors, and it potentially plays a role in determining fetal–maternal ABO incompatibility. Keywords: ABO grouping, Adsorption-elution test, SNaPshot-based assays, Rare ABO blood groups, Fetal–maternal ABO incompatibility, Single nucleotide polymorphisms (SNPs)http://www.sciencedirect.com/science/article/pii/S0929664618301694 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ding-Ping Chen Ying-hao Wen Jang-Jih Lu Ching-Ping Tseng Wei-Ting Wang |
spellingShingle |
Ding-Ping Chen Ying-hao Wen Jang-Jih Lu Ching-Ping Tseng Wei-Ting Wang Rapid rare ABO blood typing using a single PCR based on a multiplex SNaPshot reaction Journal of the Formosan Medical Association |
author_facet |
Ding-Ping Chen Ying-hao Wen Jang-Jih Lu Ching-Ping Tseng Wei-Ting Wang |
author_sort |
Ding-Ping Chen |
title |
Rapid rare ABO blood typing using a single PCR based on a multiplex SNaPshot reaction |
title_short |
Rapid rare ABO blood typing using a single PCR based on a multiplex SNaPshot reaction |
title_full |
Rapid rare ABO blood typing using a single PCR based on a multiplex SNaPshot reaction |
title_fullStr |
Rapid rare ABO blood typing using a single PCR based on a multiplex SNaPshot reaction |
title_full_unstemmed |
Rapid rare ABO blood typing using a single PCR based on a multiplex SNaPshot reaction |
title_sort |
rapid rare abo blood typing using a single pcr based on a multiplex snapshot reaction |
publisher |
Elsevier |
series |
Journal of the Formosan Medical Association |
issn |
0929-6646 |
publishDate |
2019-01-01 |
description |
Background: ABO subgroups would be considered when discrepancies in ABO grouping occur. Serological methods including adsorption-elution test, salivary ABH inhibition test, and anti-A1 (lectin) saline method could be used. However, these serological methods are laboring and obscure. Therefore, reliable and affordable method to assess the ABO subgroups is of particular interest. Methods: To solve this problem, the multiplex SNaPshot-based assays were designed to determine rare A and B subgroups. Primers used as probes for determination of rare ABO blood groups known in Taiwanese population were designed. Many ABO subtype samples were used to validate the accuracy and reproducibility of our SNaPshot panel. Results: A panel of primer probes were successfully designed in determining 8 SNP sites (261, 539, 838, 820, 745, 664, IVS6 +5, and 829 in exon 6 and 7) for A phenotype and 6 SNP sites (261, 796, IVS3 +5, 247, 523, and 502 in exon 2, 6 and 7 and intron 3) for B phenotype. SNaPshot analysis for defining blood group A alleles (A1, A2, A3, Am and Ael) and blood group B alleles (B1, B3, Bw and Bel) was therefore available. Conclusion: SNaPshot analysis could be used in reference laboratories for typing known rare subgroups of A and B without DNA cloning and traditional sequencing. Moreover, this method would help to construct databases of genotyped blood donors, and it potentially plays a role in determining fetal–maternal ABO incompatibility. Keywords: ABO grouping, Adsorption-elution test, SNaPshot-based assays, Rare ABO blood groups, Fetal–maternal ABO incompatibility, Single nucleotide polymorphisms (SNPs) |
url |
http://www.sciencedirect.com/science/article/pii/S0929664618301694 |
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