Donor Preconditioning with Inhaled Sevoflurane Mitigates the Effects of Ischemia-Reperfusion Injury in a Swine Model of Lung Transplantation
Primary graft dysfunction (PGD) and ischemia-reperfusion injury (IRI) occur in up to 30% of patients undergoing lung transplantation and may impact on the clinical outcome. Several strategies for the prevention and treatment of PGD have been proposed, but with limited use in clinical practice. In th...
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doaj-696a1451ea3748fc9fc247ce1e9628b72021-02-15T12:52:43ZengHindawi LimitedBioMed Research International2314-61332314-61412021-01-01202110.1155/2021/66259556625955Donor Preconditioning with Inhaled Sevoflurane Mitigates the Effects of Ischemia-Reperfusion Injury in a Swine Model of Lung TransplantationAlessandro Bertani0Vitale Miceli1Lavinia De Monte2Giovanna Occhipinti3Valeria Pagano4Rosa Liotta5Ester Badami6Fabio Tuzzolino7Antonio Arcadipane8Division of Thoracic Surgery and Lung Transplantation, Department for the Treatment and Study of Cardiothoracic Diseases and Cardiothoracic Transplantation, IRCCS-ISMETT, Palermo, ItalyResearch Department, IRCCS-ISMETT, Palermo, ItalyDivision of Thoracic Surgery and Lung Transplantation, Department for the Treatment and Study of Cardiothoracic Diseases and Cardiothoracic Transplantation, IRCCS-ISMETT, Palermo, ItalyDepartment of Anesthesiology and Critical Care, IRCCS-ISMETT, Palermo, ItalyFondazione Ri.MED, Palermo, ItalyDepartment of Pathology, IRCCS-ISMETT, Palermo, ItalyFondazione Ri.MED, Palermo, ItalyResearch Department, IRCCS-ISMETT, Palermo, ItalyDepartment of Anesthesiology and Critical Care, IRCCS-ISMETT, Palermo, ItalyPrimary graft dysfunction (PGD) and ischemia-reperfusion injury (IRI) occur in up to 30% of patients undergoing lung transplantation and may impact on the clinical outcome. Several strategies for the prevention and treatment of PGD have been proposed, but with limited use in clinical practice. In this study, we investigate the potential application of sevoflurane (SEV) preconditioning to mitigate IRI after lung transplantation. The study included two groups of swines (preconditioned and not preconditioned with SEV) undergoing left lung transplantation after 24-hour of cold ischemia. Recipients’ data was collected for 6 hours after reperfusion. Outcome analysis included assessment of ventilatory, hemodynamic, and hemogasanalytic parameters, evaluation of cellularity and cytokines in BAL samples, and histological analysis of tissue samples. Hemogasanalytic, hemodynamic, and respiratory parameters were significantly favorable, and the histological score showed less inflammatory and fibrotic injury in animals receiving SEV treatment. BAL cellular and cytokine profiling showed an anti-inflammatory pattern in animals receiving SEV compared to controls. In a swine model of lung transplantation after prolonged cold ischemia, SEV showed to mitigate the adverse effects of ischemia/reperfusion and to improve animal survival. Given the low cost and easy applicability, the administration of SEV in lung donors may be more extensively explored in clinical practice.http://dx.doi.org/10.1155/2021/6625955 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alessandro Bertani Vitale Miceli Lavinia De Monte Giovanna Occhipinti Valeria Pagano Rosa Liotta Ester Badami Fabio Tuzzolino Antonio Arcadipane |
spellingShingle |
Alessandro Bertani Vitale Miceli Lavinia De Monte Giovanna Occhipinti Valeria Pagano Rosa Liotta Ester Badami Fabio Tuzzolino Antonio Arcadipane Donor Preconditioning with Inhaled Sevoflurane Mitigates the Effects of Ischemia-Reperfusion Injury in a Swine Model of Lung Transplantation BioMed Research International |
author_facet |
Alessandro Bertani Vitale Miceli Lavinia De Monte Giovanna Occhipinti Valeria Pagano Rosa Liotta Ester Badami Fabio Tuzzolino Antonio Arcadipane |
author_sort |
Alessandro Bertani |
title |
Donor Preconditioning with Inhaled Sevoflurane Mitigates the Effects of Ischemia-Reperfusion Injury in a Swine Model of Lung Transplantation |
title_short |
Donor Preconditioning with Inhaled Sevoflurane Mitigates the Effects of Ischemia-Reperfusion Injury in a Swine Model of Lung Transplantation |
title_full |
Donor Preconditioning with Inhaled Sevoflurane Mitigates the Effects of Ischemia-Reperfusion Injury in a Swine Model of Lung Transplantation |
title_fullStr |
Donor Preconditioning with Inhaled Sevoflurane Mitigates the Effects of Ischemia-Reperfusion Injury in a Swine Model of Lung Transplantation |
title_full_unstemmed |
Donor Preconditioning with Inhaled Sevoflurane Mitigates the Effects of Ischemia-Reperfusion Injury in a Swine Model of Lung Transplantation |
title_sort |
donor preconditioning with inhaled sevoflurane mitigates the effects of ischemia-reperfusion injury in a swine model of lung transplantation |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2021-01-01 |
description |
Primary graft dysfunction (PGD) and ischemia-reperfusion injury (IRI) occur in up to 30% of patients undergoing lung transplantation and may impact on the clinical outcome. Several strategies for the prevention and treatment of PGD have been proposed, but with limited use in clinical practice. In this study, we investigate the potential application of sevoflurane (SEV) preconditioning to mitigate IRI after lung transplantation. The study included two groups of swines (preconditioned and not preconditioned with SEV) undergoing left lung transplantation after 24-hour of cold ischemia. Recipients’ data was collected for 6 hours after reperfusion. Outcome analysis included assessment of ventilatory, hemodynamic, and hemogasanalytic parameters, evaluation of cellularity and cytokines in BAL samples, and histological analysis of tissue samples. Hemogasanalytic, hemodynamic, and respiratory parameters were significantly favorable, and the histological score showed less inflammatory and fibrotic injury in animals receiving SEV treatment. BAL cellular and cytokine profiling showed an anti-inflammatory pattern in animals receiving SEV compared to controls. In a swine model of lung transplantation after prolonged cold ischemia, SEV showed to mitigate the adverse effects of ischemia/reperfusion and to improve animal survival. Given the low cost and easy applicability, the administration of SEV in lung donors may be more extensively explored in clinical practice. |
url |
http://dx.doi.org/10.1155/2021/6625955 |
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