Reverse transcription-polymerase chain reaction (RT-PCR) based detection and economic impact of foot-and-mouth disease in District Faisalabad, Pakistan during the year 2015
The aim of this study was to evaluate the economic impact of the disease by using milk production records and to determine the serotypes circulating in the region during 2015. Sampling was done from different outbreaks initially on the basis of clinical signs and later reverse transcriptase-polymera...
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University of Mosul, College of Veterinary Medicine
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doaj-696da0ee2bee4c81ad2490b865faf3512020-11-24T21:51:09ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552017-06-0131 English116Reverse transcription-polymerase chain reaction (RT-PCR) based detection and economic impact of foot-and-mouth disease in District Faisalabad, Pakistan during the year 2015W. Ali0M. Habib1R.S.A. Khan2M.A. Zia3I.U. Khan4U. Saliha5M. Farooq6M.S. Shah7H.M. Muzammil8Department of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), IslamabadDepartment of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), IslamabadDepartment of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), IslamabadDepartment of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), IslamabadDepartment of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), IslamabadCivil Veterinary Hospital, Faisalabad, PakistanDepartment of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), IslamabadDepartment of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), IslamabadDepartment of Biological Sciences, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, affiliated with Pakistan Institute of Engineering and Applied Sciences (PIEAS), IslamabadThe aim of this study was to evaluate the economic impact of the disease by using milk production records and to determine the serotypes circulating in the region during 2015. Sampling was done from different outbreaks initially on the basis of clinical signs and later reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for the conformation of FMDV genome. Out of total 88 samples, 73 were found positive which were then serotyped into type O (n=44), Asia1 (n=18) and A (n=06). The economic impact was analyzed by recording milk loss at four affected farms. Their average milk yield was observed 9.2 liters before the onset of disease that decreased dramatically after the disease. Milk loss of 225 and 195 liters was recorded for buffalo and cattle respectively, during 70 days of the study period.http://www.vetmedmosul.org/ijvs/media/17-1-1e.pdfFMDRT real time PCReconomic impactmilk yieldtype Otype Asia1type A |
collection |
DOAJ |
language |
Arabic |
format |
Article |
sources |
DOAJ |
author |
W. Ali M. Habib R.S.A. Khan M.A. Zia I.U. Khan U. Saliha M. Farooq M.S. Shah H.M. Muzammil |
spellingShingle |
W. Ali M. Habib R.S.A. Khan M.A. Zia I.U. Khan U. Saliha M. Farooq M.S. Shah H.M. Muzammil Reverse transcription-polymerase chain reaction (RT-PCR) based detection and economic impact of foot-and-mouth disease in District Faisalabad, Pakistan during the year 2015 Iraqi Journal of Veterinary Sciences FMD RT real time PCR economic impact milk yield type O type Asia1 type A |
author_facet |
W. Ali M. Habib R.S.A. Khan M.A. Zia I.U. Khan U. Saliha M. Farooq M.S. Shah H.M. Muzammil |
author_sort |
W. Ali |
title |
Reverse transcription-polymerase chain reaction (RT-PCR) based detection and economic impact of foot-and-mouth disease in District Faisalabad, Pakistan during the year 2015 |
title_short |
Reverse transcription-polymerase chain reaction (RT-PCR) based detection and economic impact of foot-and-mouth disease in District Faisalabad, Pakistan during the year 2015 |
title_full |
Reverse transcription-polymerase chain reaction (RT-PCR) based detection and economic impact of foot-and-mouth disease in District Faisalabad, Pakistan during the year 2015 |
title_fullStr |
Reverse transcription-polymerase chain reaction (RT-PCR) based detection and economic impact of foot-and-mouth disease in District Faisalabad, Pakistan during the year 2015 |
title_full_unstemmed |
Reverse transcription-polymerase chain reaction (RT-PCR) based detection and economic impact of foot-and-mouth disease in District Faisalabad, Pakistan during the year 2015 |
title_sort |
reverse transcription-polymerase chain reaction (rt-pcr) based detection and economic impact of foot-and-mouth disease in district faisalabad, pakistan during the year 2015 |
publisher |
University of Mosul, College of Veterinary Medicine |
series |
Iraqi Journal of Veterinary Sciences |
issn |
1607-3894 2071-1255 |
publishDate |
2017-06-01 |
description |
The aim of this study was to evaluate the economic impact of the disease by using milk production records and to determine the serotypes circulating in the region during 2015. Sampling was done from different outbreaks initially on the basis of clinical signs and later reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for the conformation of FMDV genome. Out of total 88 samples, 73 were found positive which were then serotyped into type O (n=44), Asia1 (n=18) and A (n=06). The economic impact was analyzed by recording milk loss at four affected farms. Their average milk yield was observed 9.2 liters before the onset of disease that decreased dramatically after the disease. Milk loss of 225 and 195 liters was recorded for buffalo and cattle respectively, during 70 days of the study period. |
topic |
FMD RT real time PCR economic impact milk yield type O type Asia1 type A |
url |
http://www.vetmedmosul.org/ijvs/media/17-1-1e.pdf |
work_keys_str_mv |
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