Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment.

Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment.

To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced cell density as a result of cell differentiation an...

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Main Authors: Jon Celay, Idoia Blanco, Paula Lázcoz, Mirja Rotinen, Javier S Castresana, Ignacio Encío
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3641123?pdf=render
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spelling doaj-696efadf0af8401bb408e0ac46d4853b2020-11-25T01:42:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6277110.1371/journal.pone.0062771Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment.Jon CelayIdoia BlancoPaula LázcozMirja RotinenJavier S CastresanaIgnacio EncíoTo determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced cell density as a result of cell differentiation and/or apoptosis. Flow cytometry revealed that 9-cis RA induced apoptosis more effectively than ATRA. The expression profile of apoptosis and survival pathways was cell line specific and depended on the isomer used.http://europepmc.org/articles/PMC3641123?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Jon Celay
Idoia Blanco
Paula Lázcoz
Mirja Rotinen
Javier S Castresana
Ignacio Encío
spellingShingle Jon Celay
Idoia Blanco
Paula Lázcoz
Mirja Rotinen
Javier S Castresana
Ignacio Encío
Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment.
PLoS ONE
author_facet Jon Celay
Idoia Blanco
Paula Lázcoz
Mirja Rotinen
Javier S Castresana
Ignacio Encío
author_sort Jon Celay
title Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment.
title_short Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment.
title_full Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment.
title_fullStr Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment.
title_full_unstemmed Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment.
title_sort changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced cell density as a result of cell differentiation and/or apoptosis. Flow cytometry revealed that 9-cis RA induced apoptosis more effectively than ATRA. The expression profile of apoptosis and survival pathways was cell line specific and depended on the isomer used.
url http://europepmc.org/articles/PMC3641123?pdf=render
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AT idoiablanco changesingeneexpressionprofilingofapoptoticgenesinneuroblastomacelllinesuponretinoicacidtreatment
AT paulalazcoz changesingeneexpressionprofilingofapoptoticgenesinneuroblastomacelllinesuponretinoicacidtreatment
AT mirjarotinen changesingeneexpressionprofilingofapoptoticgenesinneuroblastomacelllinesuponretinoicacidtreatment
AT javierscastresana changesingeneexpressionprofilingofapoptoticgenesinneuroblastomacelllinesuponretinoicacidtreatment
AT ignacioencio changesingeneexpressionprofilingofapoptoticgenesinneuroblastomacelllinesuponretinoicacidtreatment
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