Different Motile Behaviors of Human Hematopoietic Stem versus Progenitor Cells at the Osteoblastic Niche
Despite advances in our understanding of interactions between mouse hematopoietic stem cells (HSCs) and their niche, little is known about communication between human HSCs and the microenvironment. Using a xenotransplantation model and intravital imaging, we demonstrate that human HSCs display disti...
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Series: | Stem Cell Reports |
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doaj-6970d5d7f0ca4961b38cca890915baad2020-11-24T21:45:51ZengElsevierStem Cell Reports2213-67112015-11-015569070110.1016/j.stemcr.2015.09.003Different Motile Behaviors of Human Hematopoietic Stem versus Progenitor Cells at the Osteoblastic NicheKatie Foster0François Lassailly1Fernando Anjos-Afonso2Erin Currie3Kevin Rouault-Pierre4Dominique Bonnet5Haematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UKHaematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UKHaematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UKHaematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UKHaematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UKHaematopoietic Stem Cell Laboratory, The Francis Crick Institute, London WC2A 3LY, UKDespite advances in our understanding of interactions between mouse hematopoietic stem cells (HSCs) and their niche, little is known about communication between human HSCs and the microenvironment. Using a xenotransplantation model and intravital imaging, we demonstrate that human HSCs display distinct motile behaviors to their hematopoietic progenitor cell (HPC) counterparts, and the same pattern can be found between mouse HSCs and HPCs. HSCs become significantly less motile after transplantation, while progenitor cells remain motile. We show that human HSCs take longer to find their niche than previously expected and suggest that the niche be defined as the position where HSCs stop moving. Intravital imaging is the only technique to determine where in the bone marrow stem cells stop moving, and future analyses should focus on the environment surrounding the HSC at this point.http://www.sciencedirect.com/science/article/pii/S2213671115002659 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katie Foster François Lassailly Fernando Anjos-Afonso Erin Currie Kevin Rouault-Pierre Dominique Bonnet |
spellingShingle |
Katie Foster François Lassailly Fernando Anjos-Afonso Erin Currie Kevin Rouault-Pierre Dominique Bonnet Different Motile Behaviors of Human Hematopoietic Stem versus Progenitor Cells at the Osteoblastic Niche Stem Cell Reports |
author_facet |
Katie Foster François Lassailly Fernando Anjos-Afonso Erin Currie Kevin Rouault-Pierre Dominique Bonnet |
author_sort |
Katie Foster |
title |
Different Motile Behaviors of Human Hematopoietic Stem versus Progenitor Cells at the Osteoblastic Niche |
title_short |
Different Motile Behaviors of Human Hematopoietic Stem versus Progenitor Cells at the Osteoblastic Niche |
title_full |
Different Motile Behaviors of Human Hematopoietic Stem versus Progenitor Cells at the Osteoblastic Niche |
title_fullStr |
Different Motile Behaviors of Human Hematopoietic Stem versus Progenitor Cells at the Osteoblastic Niche |
title_full_unstemmed |
Different Motile Behaviors of Human Hematopoietic Stem versus Progenitor Cells at the Osteoblastic Niche |
title_sort |
different motile behaviors of human hematopoietic stem versus progenitor cells at the osteoblastic niche |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2015-11-01 |
description |
Despite advances in our understanding of interactions between mouse hematopoietic stem cells (HSCs) and their niche, little is known about communication between human HSCs and the microenvironment. Using a xenotransplantation model and intravital imaging, we demonstrate that human HSCs display distinct motile behaviors to their hematopoietic progenitor cell (HPC) counterparts, and the same pattern can be found between mouse HSCs and HPCs. HSCs become significantly less motile after transplantation, while progenitor cells remain motile. We show that human HSCs take longer to find their niche than previously expected and suggest that the niche be defined as the position where HSCs stop moving. Intravital imaging is the only technique to determine where in the bone marrow stem cells stop moving, and future analyses should focus on the environment surrounding the HSC at this point. |
url |
http://www.sciencedirect.com/science/article/pii/S2213671115002659 |
work_keys_str_mv |
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